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Offlinelemunhed
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Duble tub during spawn colonization?
    #4106967 - 04/28/05 02:37 PM (11 years, 7 months ago)

AFOAF is doing the shroom god's straw tek, and using the double tub idea for air exchange during casing colonization and fruiting. Should he also use to double tub during spawning colonization (5qts popcorn onto straw in 64t bin) or should he duct tape up the holes? Thx for info =)


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OfflineJazzMatazz
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Re: Duble tub during spawn colonization? [Re: lemunhed]
    #4106985 - 04/28/05 02:45 PM (11 years, 7 months ago)

you should duc-tape up the holes, and leave the top lid of. Now cover up the top of your tub with something airtight. (Like a 10l plastic garbagebag cut open) and tape it down so now air gets in.
The basic idea of colonising (like in the jars you had before) is that mycelium needs a humidity of 100% (roughly) and high co2 levels to colonise. So, what you need is no air exchange and no light.


--------------------
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Offlinelordoftheshroomz
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Re: Duble tub during spawn colonization? [Re: JazzMatazz]
    #4106999 - 04/28/05 02:51 PM (11 years, 7 months ago)

umm high co2 levels is bad for colonization. thats like saying humans benefit from breathing in and out of a balloon all day


--------------------
MY Glove Box, Automated Terrarium, and Incubator
Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest.
THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!


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Invisibleohmatic
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Re: Duble tub during spawn colonization? [Re: lordoftheshroomz]
    #4107023 - 04/28/05 02:57 PM (11 years, 7 months ago)

Quote:

lordoftheshroomz said:
umm high co2 levels is bad for colonization. thats like saying humans benefit from breathing in and out of a balloon all day




but jet it is correct as the higher co2 levels are allright during SPAWN RUN
but the fresh air makes the mycelium realise its time for fruiting.
thats why you can cover it with foil and poke some holes,
then forget about the whole thing for a week.
peace ohm :mushroom2:


--------------------
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OfflineCiv
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Re: Duble tub during spawn colonization? [Re: lordoftheshroomz]
    #4107039 - 04/28/05 03:02 PM (11 years, 7 months ago)

Quote:

umm high co2 levels is bad for colonization. thats like saying humans benefit from breathing in and out of a balloon all day





He's very right actually. High c02 levels are wonderful for colonization/spawning.


--------------------
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So, wash it well afterwards & DON'T tell them..."  -Agar


Edited by Civ (04/28/05 03:03 PM)


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Offlinelordoftheshroomz
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Re: Duble tub during spawn colonization? [Re: Civ]
    #4107121 - 04/28/05 03:23 PM (11 years, 7 months ago)

ok sorry i jus thot high co2 levels wer bad and suffocated the myc


--------------------
MY Glove Box, Automated Terrarium, and Incubator
Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest.
THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!


Edited by lordoftheshroomz (04/28/05 03:24 PM)


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Offlinelordoftheshroomz
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Re: Duble tub during spawn colonization? [Re: lordoftheshroomz]
    #4107140 - 04/28/05 03:27 PM (11 years, 7 months ago)

Spawn Run:
Relative Humidity: 90%.
Substrate Temperature: 84?F - 86?F. Thermal death limits have been established at 106?F.
Duration: 10-14 days.
CO2: 5000 - 10,000 ppm.
Fresh Air Exchanges: 0 per hour.
Type of Casing: After fully run, cover with the standard casing whose preparations described in Chapter VIII. Layer to a depth of 1-2 inches. The casing should be balanced to an initial pH of 6.8 - 7.2.


I'll admit that i was wrong. not afraid either :grin: but really tho i do apologize


--------------------
MY Glove Box, Automated Terrarium, and Incubator
Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest.
THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!


Edited by lordoftheshroomz (04/28/05 03:28 PM)


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InvisibleDexter_Morgan
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Re: Duble tub during spawn colonization? [Re: lordoftheshroomz]
    #4107191 - 04/28/05 03:47 PM (11 years, 7 months ago)

Then why do you need to take the tape of of inoculation holes?
I'm not arguing, but this seems contradictory.


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Offlinelordoftheshroomz
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Re: Duble tub during spawn colonization? [Re: Dexter_Morgan]
    #4107231 - 04/28/05 04:00 PM (11 years, 7 months ago)

thats exactly wut my logic was. i kno u need a hgih humididty btu now i can also see why higher( not high but higher) co2 levels r important. its cuz lowered co2 levels are a trigger for pinning


--------------------
MY Glove Box, Automated Terrarium, and Incubator
Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest.
THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!


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Offlinelemunhed
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Registered: 05/12/03
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Re: Duble tub during spawn colonization? [Re: lordoftheshroomz]
    #4108415 - 04/28/05 09:04 PM (11 years, 7 months ago)

You don't take the tape off the holes. You leave them on. You just sit the shit in a big tub and cover it with no air exchange (think PF jars, they have almost 0 air exchange). Thx for the help guys.


--------------------
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OfflineWronguy
Registered: 03/05/05
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Re: Duble tub during spawn colonization? [Re: lemunhed]
    #4108482 - 04/28/05 09:26 PM (11 years, 7 months ago)

I don't think an air tight container is correct. If I'm correct, while no fresh air exchange is advisable, CO2 must be allowed to escape. Excessive build up of CO2 will slow colonization.

Case in point: I am currently spawning EQ WBS to horse manure. I poked holes in the aluminum foil and covered again with another layer of aluminum foil covering the holes. I got very little growth during the first 3 days. I talked to Roadkill about it and he said to remove the top layer of foil so only the foil with the holes in it is showing.

Well I did this 2 days ago and my spawn went from 10% colonized to 65% is just 2 days. It's still going too. I should be able to case by next weekend.

Maybe it's just my particular situation, but it can't be dismissed.


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