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 Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 4 months
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 Tape covering innoc holes 
#4104588 - 04/27/05 08:29 PM (18 years, 11 months ago) |
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Should I sterilize tin foil squares placed over the taped holes? So I can put them back over the jars after I puncture the tape w/ the needle? Or is this silly as when I take the foil off it will be exposed to said airborne contams I am obsessing about?
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My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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 Crasher
αἱρετίζω
 
Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
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Re: Tape covering innoc holes [Re: Kalix]
#4104625 - 04/27/05 08:35 PM (18 years, 11 months ago) |
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Don't bother recovering them with foil. Just make sure you've got a layer of Verm on top of your Cake, which helps keep out contams. And don't poke through the tape with your syringe either. I've had problems with a freshly flamed needle melting tape, and other problems where the adhesive gunked up the needle. So lift back the tape and inoc. You're just going to remove it later. The jars will halt their growth if you leave them on and they can't breathe.
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Give me silence, water, hope;
Give me struggle, iron, volcanoes...
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Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 4 months
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Re: Tape covering innoc holes [Re: Crasher]
#4104633 - 04/27/05 08:37 PM (18 years, 11 months ago) |
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How soon after inoculation should the tape be romved?
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My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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Crasher
αἱρετίζω
Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
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Re: Tape covering innoc holes [Re: Kalix]
#4104649 - 04/27/05 08:40 PM (18 years, 11 months ago) |
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This might be superstition on my part, but I wait until a good portion of the substrate is colonized, like about 30%. If you've got a verm layer, you could leave it off immediately after inoculation.
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Give me silence, water, hope;
Give me struggle, iron, volcanoes...
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Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 4 months
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Re: Tape covering innoc holes [Re: Crasher]
#4104651 - 04/27/05 08:41 PM (18 years, 11 months ago) |
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I will have a verm layer, but superstitution sounds good for me, that is if your fairly sure that the lack of air exchange won't hurt anything?
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My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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deathbychimney
45th GenerationRoman
Registered: 02/26/05
Posts: 239
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Re: Tape covering innoc holes [Re: Kalix]
#4104654 - 04/27/05 08:41 PM (18 years, 11 months ago) |
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i taped the holes on my jars because i noted that magash had done it in his PFtek pictorial. right after i inocculated though i saw that most people suggested not doing it, and that magash explained that he did it only until he saw myc growth. i took it off after like day 3 of incubation and after that they took off. i also turned my jars upside down, which also helped it seems.
i don't think i'll tape again, it was just a pain in the ass to remove in my glovebox
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diarrhea takes over your life
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Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 4 months
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I have no glovebox, I'm relying on airborne Lysol.. So I think tape wouldn't hurt...
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My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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Crasher
αἱρετίζω
Registered: 03/13/01
Posts: 6,220
Loc: Tardy to the Party
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It won't hurt any growth for the first few days of colonization. You'll be fine. I turn mine upside down as well, usually during the last 10% when it's having trouble getting to the bottom.
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Give me silence, water, hope;
Give me struggle, iron, volcanoes...
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martin
Stranger
Registered: 12/04/03
Posts: 15
Loc: belgium, brussel
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Re: Tape covering innoc holes [Re: Crasher]
#4106041 - 04/28/05 07:26 AM (18 years, 11 months ago) |
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My 10 jars are infested by green mold, right now. It's why I'm concerned.
Is this tek okay to prevent bacterial infection during the inoculation ?
>> So, you poke hole in the lid, you put aluminum foil on it to prevent excessive water going in the substrate during the PC'ing, you put the electric tape in the PC with the jars. sterilization.
Once the jars are sterile and cool, one by one you can inoculate it and put tape (sterile) on each hole.
**Don't put tape just after the PC'ing because when cooling, the jars will contract a bit (I was having plastic jars) and some bad air will be aspired inside the jars during the inoculation (what's happened to me).**
After 3 days (or 30% of colonization), remove the tape.
is it good ?
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Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 4 months
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Re: Tape covering innoc holes [Re: martin]
#4108361 - 04/28/05 06:44 PM (18 years, 11 months ago) |
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I think you're supposed to PC with the tape on.. I sterilized (steamed, I'm doing the PF Tek) w/ the tape on. I took the tape off to innoc, and left it off. I only innoc'd yesterday, so I can't tell you if I'm succesfull yet (crosses fingers)!
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My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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Abermelin
Gnome Hat
Registered: 03/22/05
Posts: 394
Loc: In The Mycelial Network
Last seen: 18 years, 10 months
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Re: Tape covering innoc holes [Re: Kalix]
#4109345 - 04/28/05 11:37 PM (18 years, 11 months ago) |
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you ever try to pull tape off your lids after its been PC'd? its a bitch. the best advice as far as that goes is to use a folded over piece of foil in place of a lid, remembering to screw the foil down with the lid ring. no messy tape gunkin up the lid or the needle, no large hole size or guessing where your small holes are, just puncture and cap with another piece of foil.
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Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 4 months
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Re: Tape covering innoc holes [Re: Abermelin]
#4126328 - 05/03/05 12:54 AM (18 years, 11 months ago) |
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I tried the other nite.. It didn't work very well, so I just poked them w/ a sterile nail.
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My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: Tape covering innoc holes [Re: Abermelin]
#4126778 - 05/03/05 07:20 AM (18 years, 11 months ago) |
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Quote:
you ever try to pull tape off your lids after its been PC'd?
Use masking tape...grasshopper!~
its easy to pull off.
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
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Ass_Pirate
Stranger
Registered: 03/28/05
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Last seen: 18 years, 6 months
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Re: Tape covering innoc holes [Re: Roadkill]
#4128347 - 05/03/05 03:34 PM (18 years, 11 months ago) |
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or hey! put tin foil over it and p.c it and then take to glove box or sterilse area and pull tin foil off and shoot it up and put tvek over it...its free
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Tape covering innoc holes [Re: Roadkill]
#4128410 - 05/03/05 03:50 PM (18 years, 11 months ago) |
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Quote:
Roadkill said:
Quote:
you ever try to pull tape off your lids after its been PC'd?
Use masking tape...grasshopper!~
its easy to pull off.
 LOL... so true.
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
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Re: Tape covering innoc holes [Re: Kalix]
#4128935 - 05/03/05 05:44 PM (18 years, 11 months ago) |
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I recommend using no tape just foil, it's never failed me and I've also never had a stalled jar either! 
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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KaptKid
Spaced Pirate
Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 1 month
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Re: Tape covering innoc holes [Re: hyphae]
#4128965 - 05/03/05 05:52 PM (18 years, 11 months ago) |
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^^^^^^^^^^^No tape just foil.No fun taping 42 jars. Boring
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Child of the 60's, Tripping ever since.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Tape covering innoc holes [Re: KaptKid]
#4128972 - 05/03/05 05:54 PM (18 years, 11 months ago) |
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you guys are so lazy!~
lolzz
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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baraka
Registered: 07/15/00
Posts: 10,768
Loc: hyperspace 
Last seen: 2 years, 3 months
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Re: Tape covering innoc holes [Re: hyphae]
#4129062 - 05/03/05 06:21 PM (18 years, 11 months ago) |
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Taping and foil ive found to be completly unnecessary if they have the dry verm layer.
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This is the only time I really feel alive.
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