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Offlinestarspawn2
Prince of Prints
Male

Registered: 02/13/04
Posts: 21
Loc: St,louis MO
Last seen: 4 years, 6 months
Re: Transferring contams away from the mycelium [Re: RogerRabbit]
    #4113872 - 04/30/05 06:53 AM (19 years, 23 days ago)

Quote:

heres what i think. Every time you transfer you are isolating, you will be isolating untill you are down to 1 mycelium and then you would be cloning



If you start with spores cuting out even the smallest piece of mycelium is not isolating.The only way to be 100% sure is to start with a tissue from a fruit.Just expose the mycelium to light and wait for it to start pinning,it will look like bumps on the agar.Cut a small piece of the bump and transfer it to a new dish.That is the fastest way to get a isolate.
Quote:

You always run the risk of isolating a non-fruiting strain


If you use a tissue to clone the isolate will always be a fruiting stain.
Quote:

this tells me that it is probably best to start multispore in a liquid culture


No,because sectors can't be picked out or even seen.

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Offlinestarseed1066
officially hosed

Registered: 04/13/05
Posts: 325
Loc: close enough to get mysel...
Last seen: 11 years, 11 months
Re: Transferring contams away from the mycelium [Re: RogerRabbit]
    #4114014 - 04/30/05 08:31 AM (19 years, 23 days ago)

Quote:

RogerRabbit said:Do this in a glove box and not in front of a flow hood.  A flow hood will blow the contaminant spores everywhere. 




you could always do it in front of a shop vac..  :laugh:


--------------------
Under his instruction, I taped drumsticks to his head and turned him into a sort of mummy, or perhaps a caterpiller in a cocoon. He remains this way for about three hours, making bizarre noises, pretending to be a new species that must learn to walk and talk and eat, etc. And he communicates with other life forms by way of the antennae on his head. Eventually we jam a tube from a waterbong into it's mouth and figure he's learned all he needs to know.

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