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Offlinemartin
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Registered: 12/04/03
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mold or mycelium ? [1 week - PICS]
    #4098015 - 04/26/05 07:56 AM (11 years, 7 months ago)

Hi,
first thank to the experimented growers who spent their time here, I've many got good advices.

Now, I'm starting myself a little culture and when i had a look at my jars this morning, it seem that this is not really mycelium growing inside...but if you guys can say me what do you think about that, it would be cool.
It's Ecuadorian spores [cubensis] growing on rice floor + vermiculite

here is some pics :

with flash :

without :

another :

and the last one :

Thank you,

lysergication


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Offlinepsychedelic_sam
Life is shortenjoy

Registered: 11/13/04
Posts: 174
Loc: Hell in a bucket
Last seen: 9 years, 10 months
Re: mold or mycelium ? [1 week - PICS] [Re: martin]
    #4098020 - 04/26/05 08:00 AM (11 years, 7 months ago)

the first and last pics look good and white thats what you want its myc. the other pics are to dark and look discolored on my screen.


--------------------

Nothin' left to do but smile, smile, smile...;)


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Offline_JJ_
PsychedelicRevolutionary
Registered: 06/12/01
Posts: 369
Loc: NSW, Australia
Last seen: 1 year, 5 months
Re: mold or mycelium ? [1 week - PICS] [Re: psychedelic_sam]
    #4098022 - 04/26/05 08:05 AM (11 years, 7 months ago)

I have a bad feeling that you've got some green mould right there.. here's what your photo looks like with a little bit of editing:


Notice the green.. :frown:

Edit: Also, just to help you out for the future: Did you prepare the syringe yourself? How sterile were your methods? Did you P/C your jars for at least 15-20 minutes?


Edited by _JJ_ (04/26/05 08:07 AM)


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Offlinemartin
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Re: mold or mycelium ? [1 week - PICS] [Re: _JJ_]
    #4098038 - 04/26/05 08:25 AM (11 years, 7 months ago)

well, i bought the syringe from mm.org, ecuadorian strain. I,ve P/C the jars for at least 1hour.

I think that the mold have had the opportunity to go inside the jars when I've inoculated them. My jars are in plastic and when the jars where cooling, i suspect that they were contracting. And that lead to air aspiration when i've inoculated them.
fuck, how can you prevent the contamination and the air aspiration ?

thank you.


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Offline_JJ_
PsychedelicRevolutionary
Registered: 06/12/01
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Re: mold or mycelium ? [1 week - PICS] [Re: martin]
    #4098077 - 04/26/05 08:57 AM (11 years, 7 months ago)

Hmm.. tell me, is this growth around the injection site? Did you flame the needle before inserting it into each jar? Did you create a dry barrier of verm at the top? Did you wait until the jars cooled down before innoculating?


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Offlinemartin
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Re: mold or mycelium ? [1 week - PICS] [Re: _JJ_]
    #4098129 - 04/26/05 09:58 AM (11 years, 7 months ago)


is this growth around the injection site?
yes

Did you flame the needle before inserting it into each jar?
not before each jars but before the most of them.

Did you create a dry barrier of verm at the top?
yes

Did you wait until the jars cooled down before inoculating?
yes

but now, I've look at another jar and it was definitely green  :frown:

well, I think I'll buy jars in glass for my next trial and the air aspiration will be reduced.


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InvisibleColonel Kurtz Ph.D
What What?
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Registered: 07/22/04
Posts: 11,113
Loc: Shadow Moses
Re: mold or mycelium ? [1 week - PICS] [Re: martin]
    #4098193 - 04/26/05 10:36 AM (11 years, 7 months ago)

Yep, that's definetely green meanie :frown: Sorry about your loss.

Quote:

is this growth around the injection site?
yes




Oops, that means either that the needle wasn't sterile, or that your syringe was contaminated to begin with (wich is not very probable).
Remeber to flame sterilize initially and between jars with alcohol. If you keep having contams your syringe may be the problem :frown:

About the air coming inside the jars, try bulding a glovebox the next time, mine costed me less than 10? and I've only had two contams, due to bad PC'ing. :thumbup:
It's easy, cheap, and really worth it!


I wish you the best luck in your next try :mushroom2: :mushroom2:


--------------------
:whatwhat:

There's no better way to rock out than with your cock out!!


Edited by Deepman (04/26/05 10:39 AM)


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Offlinemartin
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Re: mold or mycelium ? [1 week - PICS] [Re: Colonel Kurtz Ph.D]
    #4101764 - 04/27/05 05:40 AM (11 years, 7 months ago)

Quote:

Deepman said:About the air coming inside the jars, try building a glove box the next time, mine costed me less than 10? and I've only had two contams, due to bad PC'ing. :thumbup:
It's easy, cheap, and really worth it!




well, it seem to be necessary, can you link me somewhere i can find instruction to build a glove box ?

anyway, thank you all !

I'll be luckier the next time (and more parano about bacteria :evil:)

mart.


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Amazon Shop for: ½ Pint Jars, Vermiculite

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