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Offlineblackout
Microwaver
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Sterile Pseudo Spore Prints
    #4097926 - 04/26/05 05:00 AM (12 years, 5 days ago)

I am growing some cubensis invitro in an autoclave bag. My self healing injection site is a blob of silicon on the filter patch. I plan on placing some caps on the side of the bag opposite the filter patch without opening the bag.

I can now inject sterile water through the silicon port onto the prints and suck up all the spores. These can be injected into a sterile jar which also has a injection port. I can repeat this until I have collected most of the spores, say 20 times so I now have 200ml of dense spore solution in the collection jar.

Now leave the spore solution to settle for a week or so. When the spores have settled carefully suck up the clear water from above them and discard it (or inject new cakes or keep them as syringes as some spores will remain). You should be left with an extremely dense spore solution in the bottom which you can suck up into a single syringe.

Now I get another autoclave bag and place in it 20 aluminium cupcake tins, or you could fashion little trays from foil. Seal the bag, make an injection port and sterilise.

Now inject the dense spore syringe into the bag and put 1/2cc into each cupcake. Leave the bag in a airtight container with plenty of desiccant which should suck the moisture out through the filter patch, leaving the trays dry.

While still in the bag you should be able to fold over and close your cupcake tins giving you lots of little pseudo prints.

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I have never done this, so please comment on any of the methods. I thought of this since I wanted to send some prints to the FSRE, the initial sterile syringe is an easy method, again I have not actually done it yet. Seems a cheap and easy way to get prints without a glovebox, the bag in effect becomes the glovebox. They wont have nice patterns but so what, they should work!


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Edited by blackout (04/26/05 05:03 AM)


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Offline_JJ_
PsychedelicRevolutionary
Registered: 06/12/01
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Loc: NSW, Australia
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Re: Sterile Pseudo Spore Prints [Re: blackout]
    #4097988 - 04/26/05 07:14 AM (12 years, 5 days ago)

Interesting, though it sounds like a lot of work.. why couldn't you just print the caps on the side of the bag and cut out these sections of the bag initially?
I think your idea is quite useful for making spore syringes though.

Hmm.. also leaving standing water in the bag might not be such a crash-hot idea, sterile conditions or not.

Anyhow, innovative! :smile:


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Offlineblackout
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Re: Sterile Pseudo Spore Prints [Re: _JJ_]
    #4098001 - 04/26/05 07:39 AM (12 years, 5 days ago)

It is a bit of work but so is trying to get 20 sterile prints in other ways. I would only do this to provide prints for the fsre.org you could possibly stack lots of the cases in the bag, maybe even 100. 1 print can make 100 syringes so nobody really needs a full print to start growing.

I did not want to cut the bag since it is my grow chamber for later invitro flushes (of which I will not take prints). Once opened it is not sterile (I have no fancy flowhoods), so I want the spores transfered before opening.

I did think of cutting the second bag up into prints, simply spraying it all over the bottom. But again I have no way of sterile transfer. With the cupcakes I can fold them over while still in the bag.

The only "standing water" will be 10ml of water which should be sucked up pretty quick. Can't see the problem anyway since many people store mycelium in sterile water for years.
All the water in the grow bag will be sucked up each time. When you inject 10ml it may take a few goes to suck it all back up since you will be sucking air too. You can crease teh bag so it forms a puddle and massage the spores into the water.


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