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 Roudypiper
newbie
Registered: 06/30/04
Posts: 103
Last seen: 17 years, 11 months
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 liquid culture question 
#4090034 - 04/23/05 09:57 PM (18 years, 11 months ago) |
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how much myc do u need in the jars for it to be use full, afoaf had some little jars with hardly any myc floating around, theres a lil bit in there like a buncha quarter pea size specks floating around, if he sucks what he can up in a syringe will it be enough to knock up a buncha jars. please help
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 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid culture question [Re: Roudypiper]
#4090054 - 04/23/05 10:06 PM (18 years, 11 months ago) |
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Roudypiper
newbie
Registered: 06/30/04
Posts: 103
Last seen: 17 years, 11 months
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Re: liquid culture question [Re: agar]
#4090060 - 04/23/05 10:07 PM (18 years, 11 months ago) |
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how much spore solution did u use on thoes jars
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gratefulredhead
member
Registered: 04/27/01
Posts: 488
Loc: Off the map....
Last seen: 3 years, 3 months
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Re: liquid culture question [Re: Roudypiper]
#4090133 - 04/23/05 10:36 PM (18 years, 11 months ago) |
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hey agar--is there a good liquid culture tek out there that you would recommend? what is the advantages of doing liquid culture? fast colonization? i'm a bit doubtful, just because it would, for me, add several more vectors of contamination, and that always worries me.
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HerbanShaman
I am the Walrus
Registered: 07/15/04
Posts: 233
Loc: Everywhere and Nowhere
Last seen: 18 years, 4 days
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Liquid culture do add lots of risk for contamination, but they increase colonization time outrageously. Its well worth doing, once you perfect your methods contamination won't be a problem either. Lipstick mold seems to love liquid culture, and at times it can be nearly impossible to detect. Good luck, I suggest giving it a try.
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"There is a world beyond ours, a world that is far away, nearby, and invisible. And there is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."
-- Mazatec shaman
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xburn
V card
Registered: 03/10/05
Posts: 707
Last seen: 11 years, 8 months
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Re: liquid culture question [Re: HerbanShaman]
#4090254 - 04/23/05 11:08 PM (18 years, 11 months ago) |
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about how long after inocculation are those jars Agar?
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid culture question [Re: xburn]
#4090662 - 04/24/05 01:48 AM (18 years, 11 months ago) |
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They are around a week old. Mostly started from an agar isolate wedge, or tissue, or single fully colonized WBS grain. Spores take a few days longer (germination time).
You want to work under a flow hood, in a glove box, or if you have neither - on a very sanitized bathroom counter, with mask, hair cover & gloves, with slow calm movements.
2% dry dextrose 2% light dry malt.
Mix, pour in large jar, PC 20 minutes @ 15 psi.
Remove - cool - pour through double coffee filters.
(removes any gunk, or carmelization that forms)
Pour into individual jars & PC again 20 minutes @ 15 psi.
Lids as below make it very easy to keep it sterile.
Just rubber tubing, RTV high temp silicone gasket maker & polyfil.
(you can do the same with a metal lid)
  
  
PS.... you can get good small bore rubber tubing at any auto parts store (vacuum hose for windsheild wipers)for cheap.
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backupwards
peon
Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
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Re: liquid culture question [Re: agar]
#4090917 - 04/24/05 06:46 AM (18 years, 11 months ago) |
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agar prec the info, tried a couple of times with liquid culture but always contamed. great pics helped out thanxs again. is it necessary to use the dextrose? the tek i used said only light malt at 4%. too much?
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid culture question [Re: backupwards]
#4091145 - 04/24/05 10:04 AM (18 years, 11 months ago) |
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4% is the max sugar solution you want to use. Any more becomes TOXIC to myc.
Light dry malt alone works. I use dry dextrose & light dry malt @ 2% each - because I have had great results with it. Hell, karo works.
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xburn
V card
Registered: 03/10/05
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Last seen: 11 years, 8 months
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Re: liquid culture question [Re: agar]
#4091205 - 04/24/05 10:26 AM (18 years, 11 months ago) |
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you said one tablespoon of each per quart of water right?
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jtk
Stranger
Registered: 02/06/05
Posts: 618
Last seen: 16 years, 8 months
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Re: liquid culture question [Re: agar]
#4091216 - 04/24/05 10:28 AM (18 years, 11 months ago) |
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i did the microwave tek i saw on here and used 1 Teaspoon of karo in 5 ounces of water. Is this too much sugar for the myc.? It seems to be growing decent now after 6 days at only 70 degrees.
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Don't let your brain tell your mind it can't feel
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crut939
enthusiasticnewb
Registered: 03/02/05
Posts: 100
Loc: Southeast U.S.
Last seen: 16 years, 20 days
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Re: liquid culture question [Re: jtk]
#4191616 - 05/18/05 03:29 PM (18 years, 10 months ago) |
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Exactly or approximately, how much mycelium should be in the container before it is good to put in a syringe? Mine has been incubating for 5 days and if I hold it up to the light I can see tiny strands of what looks like snot or a lugie. Probably the right stuff just doesn't look like the pics I've seen. And I know be patient, I just was curious how much was necessary.
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What I want is what I've not got...and what I need is all around me.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: liquid culture question [Re: crut939]
#4191622 - 05/18/05 03:31 PM (18 years, 10 months ago) |
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Quote:
crut939 said:
Exactly or approximately, how much mycelium should be in the container before it is good to put in a syringe? Mine has been incubating for 5 days and if I hold it up to the light I can see tiny strands of what looks like snot or a lugie. Probably the right stuff just doesn't look like the pics I've seen. And I know be patient, I just was curious how much was necessary.
when it looks like this...your good to go!~
tc
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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themills
Posts do notindicateIntelligence
Registered: 07/24/04
Posts: 177
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Re: liquid culture question [Re: agar]
#4191699 - 05/18/05 03:48 PM (18 years, 10 months ago) |
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Quote:
agar said:
Remove - cool - pour through double coffee filters.
(removes any gunk, or carmelization that forms)
I find that when using spores in a malt(or whatever) culture that since the spores sink, %90 of the time my first mycelium balls are formed, and covered in the sugary sediment. As long as it has not been carmelized I have found the sediment beneficial.
Besides, since its best to mix the malt and distilled/spring water under very low heat, why not just filter before the PC?  
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There are 2 possible outcomes: If the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery.
-Enrico Fermi
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Sibling Gatling Gun of Looking at All Sides of the Question
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crut939
enthusiasticnewb
Registered: 03/02/05
Posts: 100
Loc: Southeast U.S.
Last seen: 16 years, 20 days
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Re: liquid culture question [Re: Roadkill]
#4192687 - 05/18/05 07:37 PM (18 years, 10 months ago) |
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Ok mine doesnt look like that yet...Thanks Roadkill.
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What I want is what I've not got...and what I need is all around me.
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rasputinn
Stranger
Registered: 04/11/12
Posts: 29
Loc: oregon 
Last seen: 12 years, 5 days
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Re: liquid culture question [Re: agar]
#16084934 - 04/13/12 01:02 PM (12 years, 6 days ago) |
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i have light malt extract, Agar agar, instant potatoes and honey some say 7 gr malt extract to 200ml others say 1tbl per cup none use agar can you help, using powder light malt extract. Im not getting conatm but no culture either. I tried 1 tbl malt to 1 cup water with nothing else what am i missing here
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Luger0815
noob
Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: liquid culture question [Re: rasputinn]
#16084953 - 04/13/12 01:07 PM (12 years, 6 days ago) |
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This is my favorite recipe
20g Dry Malt Extract, or 30g (liquid)Malt Extract @1000ml Water, for LC.
20g Dry Malt Extract, or 30g (liquid)Malt Extract and 25g Agar @1000ml Water for petri dishes.
No Dextrose, potatoe or honey but you can (optional) add 2-3g Dry Yeast @1000ml for additional nutrients.
Thats it.....
If your spores dont germinate on this LC then they are maybe dead (too old or wrong storage).
Edited by Luger0815 (04/13/12 01:10 PM)
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rasputinn
Stranger
Registered: 04/11/12
Posts: 29
Loc: oregon
Last seen: 12 years, 5 days
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Re: liquid culture question [Re: Luger0815]
#16085026 - 04/13/12 01:25 PM (12 years, 6 days ago) |
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thank you i will cook it up today what is the purpose of the yeast
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Luger0815
noob
Registered: 12/21/10
Posts: 1,677
Loc: @ Home
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Re: liquid culture question [Re: rasputinn]
#16085034 - 04/13/12 01:27 PM (12 years, 6 days ago) |
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There are vitamins and other nutrients into the yeast.
The myc will benefit from them.
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rasputinn
Stranger
Registered: 04/11/12
Posts: 29
Loc: oregon
Last seen: 12 years, 5 days
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Re: liquid culture question [Re: rasputinn]
#16085052 - 04/13/12 01:31 PM (12 years, 6 days ago) |
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spores new from sporedepot in fridge for 5 days per haps not right forum but new to forum just came back to the world after five years of solitude in northren woods, is the sporedepot reliable
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