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OfflineVALIS
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Registered: 01/26/05
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Loc: neverwhere, az
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baby bottle tek - pictorialized
    #4083716 - 04/22/05 03:21 AM (12 years, 5 months ago)

When I was searching through liquid culture (LC) techniques a couple months back, I was particularly impressed with SixTango's [ a.ka. agar] Easy Liquid Culture Container Tek.

I like the use of baby bottles because of the ease and convenience of the nipple - c'mon, who doesn't love a good nipple?

Anyhow, here's what I did:




Materials - evenflo 4oz glass baby bottle, filter fiber (i.e. polyfill ), glass marble, tubing, scissors.



Snip nipple just below the tip.



Push the tubing through bottom of nipple.



Cut tubing.



Insert filter material into tubing.



Drop the marble in.



Finished product.


Further information for those who don't already know:

Liquid culture is a method where spores, an agar wedge, or mushroom tissue is injected/inserted/placed into a nutritious solution and incubated, for the purpose of inducing mycelium growth, thereby creating a large amount of inoculum that can be used to inoculate cakes or spawn with. The primary benefits of doing this are as follows:

* Lots and lots ( and lots ) of innoculant can be easily created - 1 10cc multi-spore syringe can be turned into 10 jars of LC, and 100mls of LC can create 10 syringes... pretty cool.

* Using LC to inoculate cakes or spawn can vastly speed up colonization - no lag time waiting for spore germination, in addition to the large amount of inoculation points when injected into substrate.

* The LC can be stored over long periods - throw it in your fridge for months.


Following are the beneficial aspects of using a baby bottle as a container for your LC:

* The nipple serves as a self-healing injection point.

* The nipple can be bent over or squeezed just below the filter tube in order to prevent the LC from saturating the filter when shaking or when drawing up solution.

* Easy and quick to prepare - no need to drill or seal.

* Convenient size, and it's nice to have the ml's/oz's right on the bottle.


Other things, not unique to the baby bottle tek, but useful in any LC container:

* The marble is used as means to better stir/mix the LC, and to break up any mycelium clumps that may occur.

* The filter is for gas exchange.


Finally, tomorrow or the next day I'll post a pictorial on preparing a liquid culture solution.

Hope this can be useful to folks.


Beers!


Edited by VALIS (05/07/05 03:27 PM)


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Offlinehawksapprentice
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Re: baby bottle tek - pictorialized [Re: VALIS]
    #4083726 - 04/22/05 03:26 AM (12 years, 5 months ago)

Nifty


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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Offlinescatmanrav
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Registered: 05/08/04
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Re: baby bottle tek - pictorialized [Re: VALIS]
    #4083730 - 04/22/05 03:28 AM (12 years, 5 months ago)

Baby bottles for liquid cultures, 6T obviously has no idea what hes doing.

:biggrin:


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineIGnosticAbhorI
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Registered: 11/06/04
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Re: baby bottle tek - pictorialized [Re: scatmanrav]
    #4083740 - 04/22/05 03:33 AM (12 years, 5 months ago)

Quote:

scatmanrav said:
Baby bottles for liquid cultures, 6T obviously has no idea what hes doing.

:biggrin:




:rotfl:

I just use Lc's in pint jars...Pretty much the same time (i use tyvek)....I just tape over the hole....And polyfil....if it gets wet and sits....then it becomes more of a Bridge for bacteria(contams) than as a wall...

Just some FYI

-Gnostic


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OfflineIGnosticAbhorI
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Re: baby bottle tek - pictorialized [Re: IGnosticAbhorI]
    #4083742 - 04/22/05 03:34 AM (12 years, 5 months ago)

nice pictorial btw :thumbup:

-Gnostic


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OfflineVALIS
vast activelivingintelligencesystem
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Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 5 years, 5 months
Re: baby bottle tek - pictorialized [Re: IGnosticAbhorI]
    #4083816 - 04/22/05 04:12 AM (12 years, 5 months ago)

Quote:

scatmanrav said:Baby bottles for liquid cultures, 6T obviously has no idea what hes doing. :biggrin:




Quote:

IGnosticAbhorI said:I just use Lc's in pint jars...Pretty much the same time (i use tyvek)....I just tape over the hole....





Yeah that's cool - but Real Men use plastic makeup containers pilfered from their girlfriend's bathroom drawer:




(c8=


--------------------
Nature is the Technology of the Divine.


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Offlinescatmanrav
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Registered: 05/08/04
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Re: baby bottle tek - pictorialized [Re: VALIS]
    #4083829 - 04/22/05 04:17 AM (12 years, 5 months ago)

Personally...



--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Edited by scatmanrav (04/22/05 04:27 AM)


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Invisibledeathbychimney
45th GenerationRoman
Registered: 02/26/05
Posts: 239
Re: baby bottle tek - pictorialized [Re: scatmanrav]
    #4083836 - 04/22/05 04:19 AM (12 years, 5 months ago)

what are those blue plugs? is that just poly in the other holes?

this babybottle tek sounds cool, and i've got some PESH syringes i want to try LC with

anticipating the continuation tomorrow's pictorial


--------------------
diarrhea takes over your life


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OfflineIGnosticAbhorI
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Re: baby bottle tek - pictorialized [Re: deathbychimney]
    #4083842 - 04/22/05 04:21 AM (12 years, 5 months ago)

ditto scat...ditto lol


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Re: baby bottle tek - pictorialized [Re: IGnosticAbhorI]
    #4083854 - 04/22/05 04:26 AM (12 years, 5 months ago)

Its tyvek cut out and siliconed (regular 100% silicone) over a hole. Then a blue plug is stuffed in the other holes. Peircable membranes:

https://www.sciplus.com/lw/category.cfm?category=50


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledeathbychimney
45th GenerationRoman
Registered: 02/26/05
Posts: 239
Re: baby bottle tek - pictorialized [Re: scatmanrav]
    #4083875 - 04/22/05 04:38 AM (12 years, 5 months ago)

those blue stoppers are made to be penetrated by needles and i presume seal afterwards..why the tyvek?

thanks for the link btw


--------------------
diarrhea takes over your life


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Offlinescatmanrav
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Registered: 05/08/04
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Re: baby bottle tek - pictorialized [Re: deathbychimney]
    #4083885 - 04/22/05 04:49 AM (12 years, 5 months ago)

Gas exchange of course.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineVALIS
vast activelivingintelligencesystem
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Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 5 years, 5 months
Re: baby bottle tek - pictorialized [Re: scatmanrav]
    #4083896 - 04/22/05 05:02 AM (12 years, 5 months ago)

Quote:

scatmanrav said:
Personally...






Ok, well - I guess real men can use other stuff too...




--------------------
Nature is the Technology of the Divine.


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Offlinescatmanrav
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Registered: 05/08/04
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Re: baby bottle tek - pictorialized [Re: VALIS]
    #4083902 - 04/22/05 05:07 AM (12 years, 5 months ago)

I like that gallon one...damn...


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineVALIS
vast activelivingintelligencesystem
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Registered: 01/26/05
Posts: 225
Loc: neverwhere, az
Last seen: 5 years, 5 months
Re: baby bottle tek - pictorialized [Re: scatmanrav]
    #4083905 - 04/22/05 05:16 AM (12 years, 5 months ago)

Quote:

scatmanrav said:
I like that gallon one...damn...




Yup... but dude - check out all that healthy myc in the martini glass! Now that's what I call a stiff drink.




Now, that might look like I just chucked a marble and some cotton in a water-filled martini glass real quick just to out-man scatmanrav; but, uh... well -- actualy that's what I did.

I guess I odda 'fess up to the growler and the water jug also - I'm too honest!

<grinz>



:crazy2:

Thanks for playing along scatmanrav!  (c8=


--------------------
Nature is the Technology of the Divine.


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Offlineblackout
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Re: baby bottle tek - pictorialized [Re: VALIS]
    #4083937 - 04/22/05 06:00 AM (12 years, 5 months ago)

I have never had the need for gas exchange with liquid innoc. What is very important is to shake up the jars very well after sterilising. During boiling all of the gas from the liquid is driven off. If you shake up the jar it will aerate the liquid which the mycelium needs to start growing well. In home brewing many people use areation stone after boiling up their brew prior to adding the yeast. If you use a filter on you jars you may not be able to shake them very hard for fear of soaking the filter material


--------------------
support the free spore ring http://fsre.nl/


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Offlinedstark
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Re: baby bottle tek - pictorialized [Re: VALIS]
    #11141699 - 09/28/09 05:02 AM (8 years, 25 days ago)

Such a nice TEK that i cant, not bringing it up :smile:


--------------------
What is a mind, if not something to be messed with? What is consciousness, if not a state to be altered?

~I Feel
:mushroom2:
at Home~


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InvisibleJavadog
Continuing along
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Registered: 05/03/10
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Re: baby bottle tek - pictorialized [Re: VALIS]
    #12606227 - 05/21/10 10:10 PM (7 years, 5 months ago)

You used a Dead Kennedy's disc!?!?

Jello would be proud.  ;0)


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org


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InvisibleShea25
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Registered: 01/27/09
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Re: baby bottle tek - pictorialized [Re: Javadog]
    #12606257 - 05/21/10 10:16 PM (7 years, 5 months ago)

Quote:

Javadog said:
You used a Dead Kennedy's disc!?!?

Jello would be proud.  ;0)




you bumped this thread to say that :facepalm:


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: baby bottle tek - pictorialized [Re: VALIS]
    #12606329 - 05/21/10 10:32 PM (7 years, 5 months ago)

Quote:

VALIS said:
Quote:

scatmanrav said:
I like that gallon one...damn...




Yup... but dude - check out all that healthy myc in the martini glass! Now that's what I call a stiff drink.




Now, that might look like I just chucked a marble and some cotton in a water-filled martini glass real quick just to out-man scatmanrav; but, uh... well -- actualy that's what I did.

I guess I odda 'fess up to the growler and the water jug also - I'm too honest!

<grinz>



:crazy2:

Thanks for playing along scatmanrav!  (c8=




You totally had me.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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