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Amazon Shop: ½ Pint Jars, Agar, Scales

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Offlinexburn
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I got A Liauid cultre question
    #4075010 - 04/20/05 12:44 AM (12 years, 5 months ago)

I have been working on lconing a portabella thru the use of 50/50 maltd/dextrose. Using a 1/2 tespoon to a half cup which reults in approximatley a 5-6% sugar content. My problem is not getting growth but recognizing the growth. Unfortunatley pictures cannot capture what i am about to try to describe. I get the malt formation at the bottom of my jar which is just a few brown clumps and that is due mostly to a lack of straining on my part. When the growth actually begins it never really solidifies like agars and all your guys's pictures. It stays either as a thousand separate pieces of myc or a millino particules that never develop into a colony.

My question is do you do anyhting special to get a huge myc ball or am i missing something gigantic?


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InvisibleNeedMoreSleep
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Re: I got A Liauid cultre question [Re: xburn]
    #4075093 - 04/20/05 01:07 AM (12 years, 5 months ago)

You actually want it to spread around so that it'll be easier to suck up mycelium when you need to do so.  When you have just a big ball of myc. you end up trying to break it up some anyway (unless the whole jar is a ball of myc. like some peeps we know! :wink: )  Just give it some time, and those little parts of myc. floating around should develop into larger clouds.  If they don't, then I would re-think your recipe, as you may have just a tad too much sugar content.  If my memory serves me correctly, its been found that 4% nutrient content works best.  Someone correct me if I'm wrong there...  GL bro :stoned:


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"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
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Offlinexburn
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Re: I got A Liauid cultre question [Re: NeedMoreSleep]
    #4075111 - 04/20/05 01:11 AM (12 years, 5 months ago)

yeah i due 1/4 teaspoon of each usually dont fill the spooon full though to lower the sugar back down


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Offlinescrname
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Re: I got A Liauid cultre question [Re: xburn]
    #4075113 - 04/20/05 01:12 AM (12 years, 5 months ago)

When is the LC done? When There is mycellum through out the whole jar?


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Offlinexburn
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Re: I got A Liauid cultre question [Re: xburn]
    #4075115 - 04/20/05 01:12 AM (12 years, 5 months ago)

i am just worried about seeing contams :P just a noob worry i guess


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Re: I got A Liauid cultre question [Re: scrname]
    #4075176 - 04/20/05 01:29 AM (12 years, 5 months ago)

>>>When is the LC done?

Not exactly sure what you mean here. :confused:

If you mean "done" as in ready to be used for inoculation of substrate, then once you have enough mycelium grown to innoc with it is "done".

If you mean "done" as in it cannot be used anymore, then it would have to contam or otherwise become non-viable (terminology???) before it is "done".

Either way, if things are done correctly, you can make many innoculations with a LC.  The mycelium will continue to grow even after you've taken some of the LC solution out.  I've got PR and GT liquid cultures at room temperature that are still viable 3 months later.  There's more mycelium in there now than there was the first time I used the LC to innoc, so I'm not sure if these are "done" :wink:  Don't know if that answered your question or not, I'm pretty baked right now...  GL bro :bongload:


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"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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Offlinexburn
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Re: I got A Liauid cultre question [Re: NeedMoreSleep]
    #4075271 - 04/20/05 01:46 AM (12 years, 5 months ago)

about how long does it take for a clone of tissue to start colonizing the LC

i made one and about 4 days after made it i got growth. in the for of thousands of tiny swirls


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Edited by xburn (04/20/05 01:46 AM)


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InvisibleNeedMoreSleep
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Re: I got A Liauid cultre question [Re: xburn]
    #4075286 - 04/20/05 01:49 AM (12 years, 5 months ago)

I've never actually cloned via LC, so I'm not sure on how long it takes on average.  But 4 days sounds pretty good to me, I'd be happy with those results :smile:


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"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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Invisibletahoe
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Re: I got A Liauid cultre question [Re: NeedMoreSleep]
    #4075290 - 04/20/05 01:51 AM (12 years, 5 months ago)

good growth,

Bad growth in the middle


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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Re: I got A Liauid cultre question [Re: tahoe]
    #4075299 - 04/20/05 01:54 AM (12 years, 5 months ago)

:thumbup: :thumbup:

Man that middle one looks pretty nasty... did you clone that shit growing on your back scrubber!?!? :wink:


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"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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Offlinexburn
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Re: I got A Liauid cultre question [Re: NeedMoreSleep]
    #4075312 - 04/20/05 01:57 AM (12 years, 5 months ago)

thanks tahoe

what contam is that anyway?


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Edited by xburn (04/20/05 01:58 AM)


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Offlineblackout
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Re: I got A Liauid cultre question [Re: xburn]
    #4075687 - 04/20/05 04:36 AM (12 years, 5 months ago)

Too much sugar is harmful. You would probably see more growth with 2% than 6%. 4% is ideal but if you do not have accurate scales I would aim for 2-3%. You can put some shards of broken glass in the jar prior to sterilising so you can cut up the mycelium ball by shaking it, sometimes it cannot easily go up the syringe needle if it is a big ball.


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Amazon Shop: ½ Pint Jars, Agar, Scales

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