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Amazon Shop for: ½ Pint Jars, Vermiculite

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OfflineBodybuildingElf
NewbieCultivator
Registered: 03/03/05
Posts: 2
Last seen: 11 years, 1 month
Inoculating a 2nd time.
    #4073780 - 04/19/05 08:20 PM (11 years, 10 months ago)

Hey guys. Newbie here.

For my first batch, I sterilized 18 1 pint jars and inoculated them with Cambodian strain. Only TWO are colonizing! My hypotheses: I didn't let most of the cakes cool down long enough (between 6 and 18 hours) before inoculating (thus killing the spores), OR I didn't shake up the (2) syringes well enough beforehand.

Now my real question is: I have a spare syringe of South American waiting to be used. Considering it's only been two weeks since making the cakes and none have shown any signs of contam, is it possible to inoculate the same jars a second time with success?

Expert opinions, please!


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Invisiblexamginex
Stranger

Registered: 11/27/04
Posts: 43
Loc: Left side of USA
Re: Inoculating a 2nd time. [Re: BodybuildingElf]
    #4073812 - 04/19/05 08:29 PM (11 years, 10 months ago)

My recommendation would be to dump them and start fresh using your spare syringe. Wait 24 hours for things to cool down before inoculation. What you have now could just ferment and go nowhere, so better to start off fresh than waste a syringe.


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Offlinepleco
Astralnaut

Registered: 03/09/05
Posts: 203
Last seen: 9 years, 8 months
Re: Inoculating a 2nd time. [Re: xamginex]
    #4074743 - 04/19/05 11:33 PM (11 years, 10 months ago)

I had a similar problem, they just happened to take a while, though. I am convinced the problem was the size of my vermiculite grains... they were like sand man =/


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Offlineblackout
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Registered: 07/17/00
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Re: Inoculating a 2nd time. [Re: pleco]
    #4075700 - 04/20/05 04:42 AM (11 years, 10 months ago)

I wouldnt dump them. They do take a long time to cool down and many are over-eager to get them started. I'd inject the other syringe. You should think of making some liquid innoculant, syringes are not that cheap especially if you are squirting them 4 times into 1/2pint jars


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OfflineMikeOLogical
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Registered: 01/31/04
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Re: Inoculating a 2nd time. [Re: BodybuildingElf]
    #4075710 - 04/20/05 04:50 AM (11 years, 10 months ago)

it all depends on what's more valuable to you- spores or substrate.

if your spore supply is limited, then you'd want to use the freshest substrate possible to insure good results, in which case you should dump your substrate and make more...

if spores are easier to produce than substrate, then you wouldn't want to waste potentially good substrate just to save spores...


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Offlineblackout
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Re: Inoculating a 2nd time. [Re: MikeOLogical]
    #4075908 - 04/20/05 07:51 AM (11 years, 10 months ago)

They are only 2 weeks old though, your jars may spend that long in the post!


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OfflineBodybuildingElf
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Registered: 03/03/05
Posts: 2
Last seen: 11 years, 1 month
Re: Inoculating a 2nd time. [Re: blackout]
    #4079547 - 04/21/05 01:22 AM (11 years, 10 months ago)

Maybe I should try the honey trick on the old jars, huh? That way I'll still have plenty of spores left in the fresh syringe. Oh, and this one is South American.


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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 801
Last seen: 1 month, 6 days
Re: Inoculating a 2nd time. [Re: pleco]
    #4080115 - 04/21/05 07:25 AM (11 years, 10 months ago)

I always use the fine grade vermiculite for my pf-tek style jars, and as long as you adjust the moisture content there should be no problem, but if you followed the pf-teks guide to how much water to use than than the wouldnt be enough and the jars would come out on the dry side, but you followed Anno simple minds pf-tek, than it was something else that caused Yoo jars not to germinate. But contrary to popular belief, fine vermiculite is fine for jars and casings, so
you if are just remember field capacity


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Amazon Shop for: ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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