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Invisiblefastfred
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Re: FastFred's Media Cookbook [Re: ragadinks]
    #3068022 - 08/30/04 04:55 AM (19 years, 9 months ago)

Done... Thanks for the input.


-FF

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Invisiblemycopsycho
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3068730 - 08/30/04 10:57 AM (19 years, 9 months ago)

i'm loving the update fred! good job and keep it coming!


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I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.

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Offlineragadinks
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3069751 - 08/30/04 03:18 PM (19 years, 9 months ago)

Do not know wether it's helpful but I have found recipes for antibiotics solution here.

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InvisibleATWAR
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3159164 - 09/21/04 08:27 AM (19 years, 8 months ago)

Ok, here are the results from the media I tested. As predicted, the selected media performed very poorly againsed the control MYPA (recipe from GGMM, 2% Malt). The most striking difference in performance was experienced with Ganoderma lucidum. There was a huge difference in speed of colonization between the control plate and the ?questionable? media recipes with the Reishi:



And of course, I had to test the most commonly grown mushroom here, Psilocybe cubensis. The mycelium of Ps. C. grew extremely fine, and not quite as quickly as the control:



Then there was Pleurotus ostreatus, a perfect mushroom for doing experiments with as its growth characteristics are well known and predictable. It is interesting to note that only the control and the Gelatin Agar colonized. The other two mediums failed to grow mycelium further than ?? away from the wedge?



And I also tested Trametes versicolor. It is my experience that the Turkey Tail has very aggressive mycelia, and did indeed colonize the test plates at exactly the same rate as the control. However, the mycelial mat was not nearly as thick or vigorous. The other two medias missing (forgot to take pictures) performed identically to the Nutrient Agar pictured below:




I made up a 2nd batch using Coprinus comatus and Hericium erinaceus, but that was about a week ago. I got tired of waiting so I posted these earlier results... I also left out my cookies and milk agar, which performed slightly better than these test plates (although I got pins on the ostreatus CNM plate). Anyway, there you have it. All media is not created equal, and does not perform equally well in the end. If you want to experiment, go ahead and try recipes not designed for mushroom mycelia. But, if you want to grow quick and healthy mycelium, stick to the tried and true:

Una's proven agar nutrient media recipes.

Shroomery FAQ media recipes.

Shroomery Cultivation Guide media recipes.


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To give is to live...


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Offlinehyphae
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Re: FastFred's Media Cookbook [Re: ATWAR]
    #3160187 - 09/21/04 02:12 PM (19 years, 8 months ago)

So anyone heard from FF lately? Last thing I saw was some replying to FF simply saying "DEAD" an haven't heard a word since?

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Invisiblefastfred
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3212633 - 10/04/04 02:48 AM (19 years, 8 months ago)

I'm still around... Not dead yet... Who said I was dead? That's not a very nice thing to say.

I've gotten a new job, which is why I haven't posted lately. I've been busting my ass to pay the bills and whatnot.

I'd like to thank ATWAR for doing these experiments. Great job with the photos. I'll be revising the guide at some point to make clear that these medias perform poorly. Sorry for not getting back to you, but I got too busy.

I had expected that a several media types would not perform very well. I expected them to perform slightly better than they did though. Some of my imagined uses for some of the poorly performing medias seem much less usefull now that I've thought about them more.

I'll be doing some experiments relating to screening for auxotrophs in the comming months (hopefully), so if any media proves usefull in that respect, I'll add it to the guide.


-FF

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Offlineragadinks
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3212695 - 10/04/04 03:52 AM (19 years, 8 months ago)

> I'll be doing some experiments relating to screening for auxotrophs in the comming months (hopefully), so if any media proves usefull in that respect, I'll add it to the guide.
What does 'auxotrophs' mean ?

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Invisiblefastfred
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3215064 - 10/04/04 05:44 PM (19 years, 8 months ago)

An auxotroph is a mutant that can't grow in simple media. Usually it is a result of a mutation that knocks out a metabolism pathway. The result is that an auxotroph is unable to synthesize a certain amino acid, so it must be present in the substrate or it will be unable to grow.

They are mainly useful for mating experiments. If you have two auxotrophs of different strains they will likely be deficient in different areas, hence any fusion of the two will result in an organism that is nutritionally competent. It's a way to screen for successful matings and/or successful completion of the parasexual cycle.


-FF

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Offlinehyphae
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3215255 - 10/04/04 06:30 PM (19 years, 8 months ago)

Good to hear your still around FF :wink:


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineCiv
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Re: FastFred's Media Cookbook (v0.97) Now 60 Recipes, Indexed! [Re: fastfred]
    #3561929 - 12/30/04 03:07 PM (19 years, 5 months ago)

I have a question about the Oatmeal Flake Agar.

Quote:

Oatmeal Flake Agar
-------------------
75 g Oatmeal flakes
20-25 g Agar
1 L water

Stir for 5-10 minutes then filter out the larger particles by pouring it through some mesh, save the broth. [Then add the agar]





This is done post-PC correct? And do I grind up the oatmeal at all? Thanks.


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"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar

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Offlinejeff01
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Re: FastFred's Media Cookbook (v0.97) Now 60 Recipes, Indexed! [Re: Civ]
    #3563439 - 12/30/04 10:43 PM (19 years, 5 months ago)

great suff, thanks!


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"impossible to walk in this muck"

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OfflineSnarp
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Re: FastFred's Media Cookbook (v0.97) Now 60 Recipes, Indexed! [Re: jeff01]
    #3699877 - 01/29/05 08:09 PM (19 years, 4 months ago)

Civ: no and no.

FastFred: thanks. Too bad the rest of this thread was such a waste of time. Can't say I'm impressed by the maturity levels around here.

--Snarp

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InvisibleIntelligentMind
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Re: FastFred's Media Cookbook [Re: fastfred]
    #3802575 - 02/19/05 03:51 AM (19 years, 3 months ago)

Happen to have that invitro agar recipe that Una uses for their salvia divinorum?! Now that, i'd like to know...

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OfflineAnnoA
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Re: FastFred's Media Cookbook [Re: IntelligentMind]
    #3802579 - 02/19/05 04:03 AM (19 years, 3 months ago)

The recipes for plant media differ very much from what you would use for mushrooms.

An excellent book on this topic is  Plants from Test Tubes : An Introduction to Micropropagation

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InvisibleIntelligentMind
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Re: FastFred's Media Cookbook [Re: Anno]
    #3802604 - 02/19/05 04:32 AM (19 years, 3 months ago)

Thanks a lot, i'll be sure to check that book out.

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OfflineAnnoA
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Re: FastFred's Media Cookbook [Re: IntelligentMind]
    #3802637 - 02/19/05 05:11 AM (19 years, 3 months ago)


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InvisibleIntelligentMind
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Re: FastFred's Media Cookbook [Re: Anno]
    #3805757 - 02/20/05 01:56 AM (19 years, 3 months ago)

*bows down to you* Good shit man! Thank you! Anno, you need any salvia cuttings? I'll send you one as a thank you. Peace man..

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OfflineMycoJunkie
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Re: FastFred's Media Cookbook [Re: IntelligentMind]
    #4069426 - 04/18/05 05:04 PM (19 years, 1 month ago)

bump


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:cussing::whip:

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