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Offlineymhrswrider
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Registered: 08/04/04
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Can you use straight H2O2 to "sterilize" a casing without killing it?
    #4065929 - 04/17/05 06:22 PM (11 years, 7 months ago)

I was thinking of using a casing I've got that is at the end of it's life as spawn to poo. I want to make sure that the casing is as clean as possible before I do this though so I don't get any contams that might be on there and waste my time. If I use the H2O2 that you get out of the pharmacy(10% concentration I think), will it kill of the myc? Should I dilute it at all? I know I've heard of people doing this. Anyone see any problems with my idea?


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Offlinepsilocyben
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Registered: 12/03/04
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Re: Can you use straight H2O2 to "sterilize" a casing without killing it? [Re: ymhrswrider]
    #4065933 - 04/17/05 06:24 PM (11 years, 7 months ago)

bad idea


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InvisibleHolydiver
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Re: Can you use straight H2O2 to "sterilize" a casing without killing it? [Re: ymhrswrider]
    #4065969 - 04/17/05 06:42 PM (11 years, 7 months ago)

Yeah big problem, it will roast your mycelium. I don't even like using low dilutions of the stuff directly on my casings.


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OfflineIGnosticAbhorI
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Re: Can you use straight H2O2 to "sterilize" a casing without killing it? [Re: Holydiver]
    #4066056 - 04/17/05 07:13 PM (11 years, 7 months ago)

Just put it in a gladware container and stick it in the microwave for like 7-8 mintues on high....Let cool and then it's ready...:shrug:

-Gnostic


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Offlinefallingaway24
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Registered: 12/25/04
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Re: Can you use straight H2O2 to "sterilize" a casing without killing it? [Re: IGnosticAbhorI]
    #4066512 - 04/17/05 10:08 PM (11 years, 7 months ago)

ya horrible idea, i accidently misted a cake once with a H2O2 sollution because i thought i had straight water in there and my whole cake turned dark as blue for like 2 days, luckily after about 2 days it returned back to its orriginal color, i got like 4 decent sized mushrooms off of it and it wouldn't do any more flushes after that.


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OfflineAbermelin
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Registered: 03/22/05
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Re: Can you use straight H2O2 to "sterilize" a casing without killing it? [Re: fallingaway24]
    #4066957 - 04/18/05 12:51 AM (11 years, 7 months ago)

heh, that sucks. i just misted my casing with a 3:1 ( 30%) solution. i also have a small plant in the container, and when i went to water the plant with some of the solution, it started to make that sizziling noise like when you put h202 on wounds. im starting to second guess the use of h202 in my misting solution. maybe a 10:1 ratio would be better.


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Offlinegratefulredhead
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Re: Can you use straight H2O2 to "sterilize" a casing without killing it? [Re: Abermelin]
    #4066968 - 04/18/05 12:55 AM (11 years, 7 months ago)

I once mis-sprayed a bleach solution on a casing. That was a disheartening and stupid mistake.... ;-) mark my bottles real GOOD now...


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OfflineMindzpore
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Re: Can you use straight H2O2 to "sterilize" a casing without killing it? [Re: gratefulredhead]
    #4067878 - 04/18/05 06:27 AM (11 years, 7 months ago)

...10%.... it will most likely injure the suface layer of the cake... but then again.. thats where any contaminants would be, right?

say you let it soak in 10% h2o2 for 10-15 minutes, that should be enough for a reasonable level of sterlilization, and the surface of the cake will certainly be less than pleased.

however, the core of the cake should still be ok. and that should provide more than enough material for the transfer.

go ahead and try. ive never done it like that. but why not, it might work and there is only one way to find out.

if it works... the more power to you, if it doesnt... you learn something. its not like there is a huge effort or cost involved.


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Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
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