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OfflineDatrutankdogg69
Cyan Man
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Registered: 01/12/01
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Loc: Pacific NW, Or.
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Please help!!Sterilization???'s
    #406521 - 09/27/01 07:05 PM (16 years, 10 months ago)

Where do I find info on h2o & microwave sterilization procedures???

Later,
P-DZ

I'm still debatin', Standin' in front of a Babtist Churh masterbatin' an prayin' ta Satan... "Bizarre"

Edited by Datrutankdogg69 on 09/27/01 06:25 PM.



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OfflineChemical_Smile
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Registered: 09/20/01
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Loc: coming down fast, miles a...
Last seen: 14 years, 9 months
Re: Please help!!Sterilization???'s [Re: Datrutankdogg69]
    #406897 - 09/27/01 11:29 PM (16 years, 10 months ago)

Search didn't bring up anything?

"For democracy any man would give his only begotten son"


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OfflineGroove_N
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Registered: 09/22/01
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Re: Please help!!Sterilization???'s [Re: Datrutankdogg69]
    #407077 - 09/28/01 01:44 AM (16 years, 10 months ago)

What's your question?
I use the microwave alot.



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OfflineBeppoMarx
old hand
Registered: 09/01/01
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Re: Please help!!Sterilization???'s [Re: Groove_N]
    #407363 - 09/28/01 11:18 AM (16 years, 10 months ago)

me too. thats about my only sterilization besides H2O2 and flaming a needle.



--------------------
Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!


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Offlinekatie_rocks
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Registered: 09/25/01
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Re: Please help!!Sterilization???'s [Re: BeppoMarx]
    #407373 - 09/28/01 11:30 AM (16 years, 10 months ago)

in attempt to streamline my production of eddible legal mushrooms, i want to use tupperware containers, ~5"x5"x3"deep
in place of bell jars to make large colonized cakes that i will then break up into a pan and case.

i autoclaved the rubbermaid cause it's supposed to be able to take the heat, but the pressure did it in.
so i put the substrate that had already been autoclaved into the rubbermaid containers and then microwaved for about 10min each, had to leave a corner unsealed so that it wouldn't explode.
just about everyone i did got contaminated.

what should i do in the future?

also, could i use microwaveable, disposable zip-lock bags, sterilize the substrate, innoculate by slightly opening the seal, innoculating with syringe, then closing?

thanks



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OfflineGroove_N
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Re: Please help!!Sterilization???'s [Re: katie_rocks]
    #407405 - 09/28/01 12:12 PM (16 years, 10 months ago)

Katie,
I have never had a problem with contam with the microwave. I use tupperware and pyrex. 8 min on high. I use verm with BRF and a layer of dry verm on top. I then use the oven tek to inoculate.
What was your substrate?
Did the substrate contam, or the casings?
How did you inoculate the substrate?
What percautions did you take while inoculating?
<<also, could i use microwaveable, disposable zip-lock bags, sterilize the substrate, innoculate by slightly opening the seal, innoculating with syringe, then closing? >>
Yes, that could work.



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Offlinepinetree
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Registered: 10/04/01
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Loc: Massachusetts
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Re: Please help!!Sterilization???'s [Re: katie_rocks]
    #413438 - 10/04/01 09:58 AM (16 years, 10 months ago)

I'm on my first attempt with the microwave tek and have found that so far putting a layer of saran wrap over the Rubbermaid container, poking tiny hole in the middle of the wrap, and lightly putting the cover on top has been fairly effective. Nuke for eight minutes. I've had one contam out of six containers 2.5 weeks later.



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OfflineDatrutankdogg69
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Registered: 01/12/01
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Re: Please help!!Sterilization???'s [Re: Groove_N]
    #413917 - 10/04/01 06:57 PM (16 years, 10 months ago)

Groov n...

That last idea sounds really good... I'm gonna try that with brown's rice recipe

Later,
P-DZ

I'm still debatin', Standin' in front of a Babtist Churh masterbatin' an prayin' ta Satan... "Bizarre"


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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