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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 10 years, 11 days
how to do grains without the need of sterilization.
    #4046045 - 04/12/05 01:28 PM (19 years, 1 month ago)

Hey you guys, this may not workin the slightest but.

when working with popcorn one can let it soak for over a week, and i know that one could just let it sit for a few days without contaminating. well, i injected liquid culture on sum pressure cooked corn the other day and in less than 24 hours the spots i sprayed had myc. on them.


i was thinking that if you just prepared a buuuunch of LC and then soaked the corn in it for a day,a dn then just let it chill out, would it colonize nicely?




something to try out soon.


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OfflineAeolus1369
Dr. Seahorse
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Registered: 05/20/02
Posts: 367
Last seen: 15 years, 3 months
Re: how to do grains without the need of sterilization. [Re: 4hodmt]
    #4046118 - 04/12/05 01:39 PM (19 years, 1 month ago)

Ehhh, probably not. If LC is based on a sugary fluid then as soon as you add unsterilized popcorn to it you're going to get an explosion of bacteria for one. I'm sure there's a dozen other reasons this wouldn't work 99 times out of 100.

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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 10 years, 11 days
Re: how to do grains without the need of sterilization. [Re: Aeolus1369]
    #4046235 - 04/12/05 02:04 PM (19 years, 1 month ago)

well im still gonna try it.

the water wouldnt be very nutritious. the reason it'd hav a ton of myc. is u makea thick old LC then transfer a ton of it tot he new one to get a milk mostly water type thing.



i think i'll work on fine tuning this.


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all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


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Invisibleohmatic
searcher
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Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: how to do grains without the need of sterilization. [Re: 4hodmt]
    #4046323 - 04/12/05 02:21 PM (19 years, 1 month ago)

soaking a week is almost 100% going to germ the corn itself.
i doubt you to be able to somehow get it working b/c there sure
have been tonst of real mycologists before you wanting to get it working aswell...
peace ohm :mushroom2:


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 10 years, 11 days
Re: how to do grains without the need of sterilization. [Re: ohmatic]
    #4047240 - 04/12/05 06:16 PM (19 years, 1 month ago)

eh, ill still try. you guys can't sway me, bwahaha


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all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


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Offlinemattymonkey
Feel Like aStranger...
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Registered: 11/07/04
Posts: 973
Last seen: 11 years, 4 months
Re: how to do grains without the need of sterilization. [Re: 4hodmt]
    #4047299 - 04/12/05 06:32 PM (19 years, 1 month ago)

good

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OfflineShortyMcAffy
n00blar

Registered: 01/01/05
Posts: 71
Loc: Pennsylvania USA
Last seen: 2 years, 2 months
Re: how to do grains without the need of sterilization. [Re: mattymonkey]
    #4047664 - 04/12/05 08:19 PM (19 years, 1 month ago)

Get to it brotha and let us know how it goes.


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Offlineblackout
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Registered: 07/16/00
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Re: how to do grains without the need of sterilization. [Re: ShortyMcAffy]
    #4049048 - 04/13/05 03:40 AM (19 years, 1 month ago)

Intresting idea. Has anybody ever tried adding a large amount of colonised grain to a small amount of uncolonised pastuerised or simply soaked grain? You could add more a few days later in higher proportions since your colonised grain will be larger each time too.

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Offlinedebianlinux
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Re: how to do grains without the need of sterilization. [Re: blackout]
    #4049639 - 04/13/05 09:12 AM (19 years, 1 month ago)

Quote:

blackout said:
Intresting idea. Has anybody ever tried adding a large amount of colonised grain to a small amount of uncolonised pastuerised or simply soaked grain? You could add more a few days later in higher proportions since your colonised grain will be larger each time too.




if i have a large amount of colonised grain i'm not going to do anything with it but spawn it to a bulk substrate. i have, however, used a small amount of colonised grain to inoculate a larger mass of uncolnised but PC'd grain.

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OfflineAeolus1369
Dr. Seahorse
Male

Registered: 05/20/02
Posts: 367
Last seen: 15 years, 3 months
Re: how to do grains without the need of sterilization. [Re: debianlinux]
    #4049773 - 04/13/05 10:22 AM (19 years, 1 month ago)

Quote:

debianlinux said:
Quote:

blackout said:
Intresting idea. Has anybody ever tried adding a large amount of colonised grain to a small amount of uncolonised pastuerised or simply soaked grain? You could add more a few days later in higher proportions since your colonised grain will be larger each time too.




if i have a large amount of colonised grain i'm not going to do anything with it but spawn it to a bulk substrate. i have, however, used a small amount of colonised grain to inoculate a larger mass of uncolnised but PC'd grain.




Word. Might be interesting as a sort of proof-of-concept experiment but the ability to do this wouldn't really be productive

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Offlinegbhtrfv
journeyman
Registered: 02/09/04
Posts: 154
Last seen: 18 years, 18 days
Re: how to do grains without the need of sterilization. [Re: Aeolus1369]
    #4062464 - 04/16/05 01:17 PM (19 years, 1 month ago)

IT WILL NOT WORK. guaranteed- 100%

when you soak the grains, you're germinating the bacterial endospores to kill them through pressure cooking. if you skip the sterilization process then you just end up with a shit load of infected corn. mycelium won't even have a chance to get a footholding on the grain because it's all covered up by bacteria.

you can still try though.

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