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Amazon Shop for: ½ Pint Jars, Agar, HEPA Filter, Rye Grain

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Offlinefirsttimer101
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Can't believe my horrible luck...at a loss for words
    #4045131 - 04/12/05 12:35 PM (11 years, 7 months ago)

Yet another contamination screw up, I really don't get what I did wrong!!! I know this is a long post, but I really am desperate for advice and would love to find a solution to my contamination problem.

I put 1 cup of rye with 11/2 cups of water in 1 litre jars. I soaked for 24 hours, rinsed and strained, simmered for 45 minutes, PCed for 1 hour and 15 minutes (with 15 minutes to build up to 15 PSI), let the jars cool, then inoculated.

I inoculated in a room that had been sprayed down with Lysol, the counter had been wiped down with alcohol, the scalpel was heated with a mini-torch and soaked in alcohol, I was wearing a mask & gloves and clean clothes and had just showered.

The lids have one hole in them with polyfill (fresh out of the bag). The jars were incubated at 85F for 7 days in the dark.

Out of 10 jars, no contamination for the first 7 days. Everything was great, the jars were almost completely permeated. Then today, I notice that EVERY JAR is contaminated with a red-coloured contaminant (I think is lipstick mold).

What could I have done wrong??? This is my 5th try at growing and still NO SHROOMS. I'm about ready to call it quits and return to my previous green growing.


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OfflineShroomGuy86
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Registered: 02/06/05
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- [Re: firsttimer101]
    #4045147 - 04/12/05 12:38 PM (11 years, 7 months ago)

-


--------------------
"I put Lamborghini doors on that Es-co-lade
Low pro so low look like I'm riding on blades" - 50 Cent




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Offlinegoofy98
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Re: Can't believe my horrible luck...at a loss for words [Re: firsttimer101]
    #4045155 - 04/12/05 12:39 PM (11 years, 7 months ago)

if every jar contaiminated and you PCed everything i would say it was the syringe.


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OfflineDarkie
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Re: Can't believe my horrible luck...at a loss for words [Re: goofy98]
    #4045160 - 04/12/05 12:41 PM (11 years, 7 months ago)

Mold or perhaps a lil rust? I once thought i had this orangish looking contam but i figured out it was just some oxidation after a while. I rarely get contams and i'm a dirty motha fuker.


--------------------
You gotta wake up to get faded but you gotta get up to get paided.


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Offlinefirsttimer101
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Re: Can't believe my horrible luck...at a loss for words [Re: goofy98]
    #4045163 - 04/12/05 12:42 PM (11 years, 7 months ago)

I used slices of agar to inoculate, not a syringe. The agar showed no signs whatsoever of contamination... I just don't get it. Also, there was no contamination for the first 7 days...if the contamination had come from the agar the contamination would have shown up earlier. I'm about ready to quit this hobby..it started out being fun but now it's just frustrating.


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InvisibleNeedMoreSleep
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Re: Can't believe my horrible luck...at a loss for words [Re: firsttimer101]
    #4045165 - 04/12/05 12:42 PM (11 years, 7 months ago)

what are your inoculation procedures???


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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InvisibleNeedMoreSleep
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Re: Can't believe my horrible luck...at a loss for words [Re: NeedMoreSleep]
    #4045177 - 04/12/05 12:46 PM (11 years, 7 months ago)

If you're using agar wedges to innoc. then you really should be working with a flowhood or at least in a glovebox.  Lysoling everything, and cleaning your counters is really only going to help prevent contams when innoc'ing w/ a syringe.  If you've gotta open up your jars to innoc. you really need to have one of the 2 before-mentioned tools...  If you can't get one of those, at least get a $30 hepa filter/air purifier, and run it in your bathroom for 45-60 mins beforehand and innoc in there.... GL bro :stoned:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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OfflineDarkie
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Re: Can't believe my horrible luck...at a loss for words [Re: NeedMoreSleep]
    #4045181 - 04/12/05 12:46 PM (11 years, 7 months ago)

Agar i have seen has red dye in it. Maybe Shroomguy was right on the money the whole time.


--------------------
You gotta wake up to get faded but you gotta get up to get paided.


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Offlinegoofy98
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Re: Can't believe my horrible luck...at a loss for words [Re: Darkie]
    #4045200 - 04/12/05 12:49 PM (11 years, 7 months ago)

I hope thats it!!! Lipstick mold is pretty rare.


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Offlinefirsttimer101
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Re: Can't believe my horrible luck...at a loss for words [Re: goofy98]
    #4045229 - 04/12/05 12:55 PM (11 years, 7 months ago)

Wouldn't red dye show up right away? I mean, it wouldn't just show up one day and continue to grow.

And I did run a HEPA/charcoal filter in the room for 1 hour before I inoculated.

I didn't use a spore syringe because I was under the impression that it was necessary to isolate a single mycelial strain on agar, then use that uniform strain to inoculate the jars. I only used the spore syringe to inoculate the original agar dish.


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InvisibleNeedMoreSleep
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Re: Can't believe my horrible luck...at a loss for words [Re: firsttimer101]
    #4045258 - 04/12/05 01:02 PM (11 years, 7 months ago)

Yes, agar is useful in isolating aggressive rhizomorphic mycelium for inoculation, but is far from required.  Seriously, I'd invest in a flowhood or at least a glovebox if you want to continue working with agar.  Otherwise, just innoc w/ MSI or do a liquid culture.  Also, I'd recommend doing things in your bathroom if you've got the room.  There's no carpet in there (which harbors everyfuckingthing imaginable), and is much easier to sterilize to an appropriate level than your kitchen/living room... GL bro :stoned:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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OfflineShroomGuy86
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- [Re: NeedMoreSleep]
    #4045340 - 04/12/05 01:14 PM (11 years, 7 months ago)

-


--------------------
"I put Lamborghini doors on that Es-co-lade
Low pro so low look like I'm riding on blades" - 50 Cent




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Offlinefirsttimer101
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Re: Can't believe my horrible luck...at a loss for words [Re: NeedMoreSleep]
    #4045472 - 04/12/05 01:39 PM (11 years, 7 months ago)

Quote:

NeedMoreSleep said:
Yes, agar is useful in isolating aggressive rhizomorphic mycelium for inoculation, but is far from required.  Seriously, I'd invest in a flowhood or at least a glovebox if you want to continue working with agar.  Otherwise, just innoc w/ MSI or do a liquid culture.  Also, I'd recommend doing things in your bathroom if you've got the room.  There's no carpet in there (which harbors everyfuckingthing imaginable), and is much easier to sterilize to an appropriate level than your kitchen/living room... GL bro :stoned:




Thanks for the tips. Can I inoculate with a syringe through the polyfill?? Having a second hole with masking tape that's briefly pulled back for inoculation seems like it would create the same problems I have now. My house is obviously pretty toxic even though it looks clean. I guess I can't allow air to get to the substrate even for a second.


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InvisibleNeedMoreSleep
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Re: Can't believe my horrible luck...at a loss for words [Re: firsttimer101]
    #4045498 - 04/12/05 01:44 PM (11 years, 7 months ago)

Yea you definitely don't want to expose your sterile substrate to open air.  I would suggest using tyvek, but polyfil works well too.  If you'll be using polyfill, just drill a hole about 3/8" in the center of the lid, and fill with polyfil.  You can innoc right through the polyfil.  If you go with the tyvek, I do it this way.  I have five 1/8" holes drilled in an "X" pattern in my lids.  I put on the lids, then the tyvek, then the metal band.  Sterilize, wait to cool, then innoc right through they tyvek, and cover with a piece of bandaid or micro tape (medial tape, found at any drug store).  This method has never once failed me, and I'm definitely not the cleanliest of people... GL bro :stoned:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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Amazon Shop for: ½ Pint Jars, Agar, HEPA Filter, Rye Grain

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