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sleepingbigguy
homosapien


Registered: 11/17/04
Posts: 148
Last seen: 2 years, 11 months
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recipe for peroxide?
#4045008 - 04/12/05 09:58 AM (19 years, 1 month ago) |
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I managed to find a recipe for preparing substrate with peroxide didn't bookmark the web page and now i can't find it it was something like 3 teaspoons of peroxide to 3.5 cups water.
can anyone give me a link to a site that has the recipe not just promoting the peroxide manual.
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Aeolus1369
Dr. Seahorse


Registered: 05/20/02
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sleepingbigguy
homosapien


Registered: 11/17/04
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Re: recipe for peroxide? [Re: Aeolus1369]
#4046258 - 04/12/05 02:08 PM (19 years, 1 month ago) |
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this recipe isn't for agar it's for bulk sub. anyway i managed to find it agian so thanks anyway
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debianlinux
Myconerd - DBK



Registered: 12/09/02
Posts: 8,334
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Quote:
sleepingbigguy said: I managed to find a recipe for preparing substrate with peroxide
you get what you ask for...
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Abermelin
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Registered: 03/22/05
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Re: recipe for peroxide? [Re: debianlinux]
#4046443 - 04/12/05 02:55 PM (19 years, 1 month ago) |
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why the hell would you use peroxide in your substrate? i thought that shit was used to kill bacteria, you know, putting it on open wounds and such.
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debianlinux
Myconerd - DBK



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Re: recipe for peroxide? [Re: Abermelin]
#4046481 - 04/12/05 03:01 PM (19 years, 1 month ago) |
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so you don't have to PC? bacteria live in substrate, you know.
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sleepingbigguy
homosapien


Registered: 11/17/04
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Re: recipe for peroxide? [Re: debianlinux]
#4046583 - 04/12/05 03:18 PM (19 years, 1 month ago) |
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yeah i know i didn't word it the best.
just experimenting with bulk subs and different methods.
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crazytalken
SELECT


Registered: 04/06/05
Posts: 122
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Abermelin, H2O2 is added to many things to keep out mold, like putting some in your spray bottle...you've never heard this before?? It will kill spores but is safe on mycelium when diluted with water.
-------------------- **"Both the psychedelic dream state and the waking psychedelic state acquire great import because they reveal to life a task: to become familiar with this dimension that is causing being, in order to be familiar with it at the moment of passing from life." -Terence McKenna
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Abermelin
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Re: recipe for peroxide? [Re: crazytalken]
#4047574 - 04/12/05 07:55 PM (19 years, 1 month ago) |
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oh, no. damn, 2 years at this and im still a noob.
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Abermelin
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Re: recipe for peroxide? [Re: Abermelin]
#4047595 - 04/12/05 08:03 PM (19 years, 1 month ago) |
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can you tell me how much peroxide i am supposed to had to a liter of water?
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debianlinux
Myconerd - DBK



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Re: recipe for peroxide? [Re: Abermelin]
#4049629 - 04/13/05 09:08 AM (19 years, 1 month ago) |
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Quote:
Abermelin said: can you tell me how much peroxide i am supposed to had to a liter of water?
how much peroxide you use depends on the application and the concentration as well as personal taste.
i only use peroxide for agar recipes and casing misting.
i assume your refering to casing misting. my personal preference is roughly 2:1 or 3:1 water:peroxide. Oh, yeah, if you use a humidifier then add peroxide to the tank each time you top it off. Say goodbye to the molds that like to live in there. I know this is applicable to ultrasonics. I haven't tried it on other types but it stands to reason that it would work just as well.
Oh, I occasionally use peroxide when tranferring wedges to grain jars. Just dip the wedge for a few seconds then drop it in.
edit: oh, yeah, i mentioned concentration. All my methods involve 3% OTC h2o2. if your using a higher concentration then you, obviously, have to adjust your recipe accordingly.
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sleepingbigguy
homosapien


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Re: recipe for peroxide? [Re: Abermelin]
#4049767 - 04/13/05 10:20 AM (19 years, 1 month ago) |
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10% of 3% h202
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Abermelin
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3:1 as in if i were to put 4 cups liquid in my mister i would put 3 cups water + 1 cup h202? just checking, i suck at math.
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debianlinux
Myconerd - DBK



Registered: 12/09/02
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Re: recipe for peroxide? [Re: Abermelin]
#4051900 - 04/13/05 07:05 PM (19 years, 1 month ago) |
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that's right.
i've read of people using 10:1.
me, i consider peroxide cheap enough to use what i choose to believe is a more effective concentration. i certainly have no empirical data on the subject.
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Mindzpore
Psychedelicious


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Re: recipe for peroxide? [Re: debianlinux]
#4053464 - 04/14/05 04:18 AM (19 years, 1 month ago) |
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whats with this bs method of calculating concentrations?
10% of 3%..? do you mean 0.3%?
or 10:1..?? where I live you can readily buy h2o2 at at least 3 different concentrations (3%, 6%, 30%) and with a little effort higher concentrations than that.
I really think it would benefit all parties if we try to conform to some form of standard. I suggest that the simplest would be the actual percentage of a pure solution. its not that hard to calculate, is it?
now I'm by no means an expert when it comes to to mycology, more of a happy hobbyist. but what I know about medicine one would use concentrations atleast 7% h2o2 to sterilize tools and surfaces.
however it seems this is "a bit" high, and solutions around 0,2-0,5 seems to be enough to deter unwanted growth in substrates, waters, agar, dunk/spraywater. but then again... as I said, I'm not a microbiologist, so i might be wrong then again... I'll most likely get "corrected" if I am (wee, learning).
-------------------- Mindzpores words of wisdom: "If you think something is foolproof, you just haven't met proper fools". Wiccan_Seeker said: "It is better to adjust to become a better listener than to keep on cranking up the volume".
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Shdwstr
FSRCanada


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Re: recipe for peroxide? [Re: Mindzpore]
#4053649 - 04/14/05 06:57 AM (19 years, 1 month ago) |
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Yes... 10% of 3%
1 cup H2O2(3%) and 9 cups Water !!!
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Abermelin
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Registered: 03/22/05
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Re: recipe for peroxide? [Re: Shdwstr]
#4054022 - 04/14/05 09:42 AM (19 years, 1 month ago) |
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are we all referring to standard h202 you buy at the store in a brown bottle? btw, 10:1 is a far shot from 3:1. thats the difference between life and death for mycellium.
someone clairfy this, i dont want to kill my cakes.
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Shdwstr
FSRCanada


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Re: recipe for peroxide? [Re: Abermelin]
#4054086 - 04/14/05 09:58 AM (19 years, 1 month ago) |
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1 cup H2O2(3%) and 9 cups Water !!!
Is this not clear? 1 Cup of 3% Hydrogen Peroxide from the store in a brown bottle. Mix with.. 9 Cups of Distilled or Spring water from a semi-clear bottle from the store.
This will give you a 10% solution of a 3% H2O2 mixture or... 0.3% solution
Can be used for Misting, mixing casings, dunking, etc. your choice. Pretty much anything except spore syringes.
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Joshua
Holoman


Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Essential Solutions 101
You may want to check this thread out.
Joshua
-------------------- The Shroomery Bookstore Great books for inquiring minds! "Life After Death is Saprophytic!"
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debianlinux
Myconerd - DBK



Registered: 12/09/02
Posts: 8,334
Loc: Over There
Last seen: 10 months, 19 days
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Re: recipe for peroxide? [Re: Abermelin]
#4054777 - 04/14/05 12:56 PM (19 years, 1 month ago) |
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Quote:
Abermelin said: are we all referring to standard h202 you buy at the store in a brown bottle? btw, 10:1 is a far shot from 3:1. thats the difference between life and death for mycellium.
someone clairfy this, i dont want to kill my cakes.
shadwstr = 10:1 debianlinux = 3:1 Joshua = 1:1
Seems we are all getting along pretty well on our respective concetrations. my original post on the topic was full of "personal choice" and "choose to believe" precisley because I've witnessed a wide variety of concentrations being used with success. basically, the more your going to be using it the lower the concentration should be. You don't want your mycelium to have to keep wasting energy decomposing peroxide that could be better spent on fruiting/colonising. if you're strictly cautiously misting casings, i'd hazard you could get by with straight 3%. for misting bare pf type cakes you definitely want to tone it down.
Edited by debianlinux (04/14/05 12:58 PM)
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Abermelin
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Re: recipe for peroxide? [Re: debianlinux]
#4055547 - 04/14/05 04:07 PM (19 years, 1 month ago) |
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straight 3%? straight brown bottle h202? i dont know about that. ive seen what that shit does to my open wounds, and i dont think i want any of that on my cakes. I think the original idea of adding h202 to the misting bottle wasnt to decontaminate the casing, although the creators of this method probably found that out, i think this was meant to keep your misting bottle from growing bacteria so you in turn dont spray your casings with a cloud of it.
you know for smaller setups it takes like a few months to go through a misting bottle, and all sorts of shit grows in it.
hmm, im debating on 3:1 or the 10:1. maybe ill make it even and do a 7:1.
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debianlinux
Myconerd - DBK



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Re: recipe for peroxide? [Re: Abermelin]
#4055692 - 04/14/05 04:38 PM (19 years, 1 month ago) |
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Quote:
Abermelin said: straight 3%? straight brown bottle h202? i dont know about that. ive seen what that shit does to my open wounds, and i dont think i want any of that on my cakes.
i was referring to lightly misting casing to kill any rogue spores that happen to land on the surface. under light with air movement the h2o2 would be decomposed before it soaked to the mycelium underneath. cakes, as i stated, are a different story. no, i wouldn't actually attempt it
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Abermelin
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Re: recipe for peroxide? [Re: debianlinux]
#4074687 - 04/19/05 09:18 PM (19 years, 1 month ago) |
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i did a 3:1 ratio in my mister, misted a small plant i have in my chamber to rehydrate it, and the plant started to sizzle. i honestly wouldnt go any more potent than 10:1. like i said before, this is only to keep your mister water sterile, not to sterile your casing, which is a really bad idea.
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