PDA tek ?'s - Advanced Mycology - Shroomery Message Board

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fiddler4u
enthusiast

Registered: 08/20/01
Posts: 282
Loc: Where the shrooms are gre...
Last seen: 22 years, 8 months

PDA tek ?'s

#403981 - 09/25/01 07:27 PM (23 years, 2 months ago)

After reading the pda tek for agar use; I have a few ?'s.
I plan on using half-pint jars as a petri dish, which I see is just fine. Now with that in mind......can I skip all the steps of transfering the liquid to other container's? I thought of mixing all of the ingre., stir it all together, then fill each jar 1/4 of the way up, and P.cook. After all this let each of the jars cool, then I can inocu. each jar with syringe, print. Is this not advisable? Also since doing it this way...does this mean I should P.cook for 25min? Afew more things....table sugar should be alright to use right? Will Pan.Cyans be ok on this stuff?
Well thats all I can think of for now, so any helpfull advise would be great.
thx
P.S. sorry for the bombardment of ?'s. Just want to get everything in order as best possible.
thx

I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5

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I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5

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Jahson
amigo de hongos

Registered: 10/09/00
Posts: 84
Last seen: 21 years, 1 month

Re: PDA tek ?'s

[Re: fiddler4u]
#404879 - 09/26/01 01:25 PM (23 years, 2 months ago)

I wouldn't skip the step of transferring. The reason is: You want to mix up a good batch if your doing it(.5 liter at least) and in order to get good accurate measurement you should do it in one container. Another reason is that you don't want to pour all that much agar solution into a jar. The mycelium is only 2-D. it doesn't penetrate the agar. why waste it on depth. If you want pyrex or kimax autoclavble petris...I have extras. I need to send a bunch out in fact.

Enjoy FSR

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Enjoy FSR

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fiddler4u
enthusiast

Registered: 08/20/01
Posts: 282
Loc: Where the shrooms are gre...
Last seen: 22 years, 8 months

Re: PDA tek ?'s

[Re: Jahson]
#404892 - 09/26/01 01:37 PM (23 years, 2 months ago)

Hey that sounds great jahson :) I'm game...

I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5

--------------------
I am the vine, you are the branches. If a man remains in me, and I in him, he will bare much fruit.
Apart from me you can do nothing. john 15:5

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darkfly
LysergicExperimenter

Registered: 09/16/01
Posts: 1,169
Last seen: 9 years, 4 months

Re: PDA tek ?'s

[Re: Jahson]
#405190 - 09/26/01 05:39 PM (23 years, 2 months ago)

How much are you lookin to sell those pyrex ones for.

darkfly

-- too much trippin and my soul is worn thin

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Everything Is Blue In This World. The Deepest Shade Of Mushroom Blue.

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Jahson
amigo de hongos

Registered: 10/09/00
Posts: 84
Last seen: 21 years, 1 month

Re: PDA tek ?'s

[Re: darkfly]
#406028 - 09/27/01 10:04 AM (23 years, 2 months ago)

PM me...I also have glass B-D syringes....I think they are beautiful...What does someone else think?

Enjoy FSR

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Enjoy FSR

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Mushrooms, Mycology and Psychedelics > Advanced Mycology

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