Home | Community | Message Board



Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars

Jump to first unread post. Pages: 1
Offlinestoner
journeyman
Registered: 03/01/01
Posts: 71
Last seen: 14 years, 8 months
amazon???
    #403841 - 09/25/01 07:04 PM (15 years, 2 months ago)

i recieved my spore print today from fsr it says its "amazon" what is this strain and is it good as far as coloniation time and overall yield? also it came on plain paper what is the easiest way to make a syringe from the print. ps. i plan on using a suction type bottle that is like a eye dropper but ten times larger. does this make it more dificult. thanks ahead of time all replys will be appreciated as i am in a hurry to start this new and wonderful project!!!!!



Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/24/00
Posts: 5,015
Re: amazon??? [Re: stoner]
    #403853 - 09/25/01 07:16 PM (15 years, 2 months ago)

How do you plan on innoculation with a large "eye dropper"?

Psilocyber's Syringe Tek v1.0

NOTE: These instructions are most effective when performed in the most sterile environment available. The preferred method involves following the steps below while working in a clean and sterile glovebox. There are many simple methods of glovebox construction; most are available on the web at the popular mycological culture websites. If you do not wish to construct a glovebox, or do not have one, the following steps have been performed with success by using the "oven tek." This simply involves using your oven as a way of reducing the amount of contamination present. To do this, turn your oven on its "warm" setting. Pull down the door. You may then use the open door as a working surface or pull out one of the oven racks halfway and work on it. The theory (unproven) is that rising heat from the oven causes airborne contaminates to rise and therefore prevents them from settling on your working surface and therefore reducing the number of contaminates present.

Materials needed:

Empty sterile syringes

Two quart (or larger) saucepan

One bottle of 91% isopropyl alcohol

Several paper towels

A lighter or alcohol flame

A shot glass

Sterile spore print Procedure One: Making a sterile syringe

1. Fill your saucepan halfway with tap or distilled water (use distilled water if your tap water contains higher levels of minerals and chemicals).

2. Boil the water in the saucepan on high for a minimum of ten minutes, this should be adequate to sterilize and cleans the water of all bacteria and viruses.

3. Take your empty syringe and fill it with the boiling water. Allow it to sit for two minutes with the hot water inside.

4. Purge the hot water from the syringe into a sink, not back into the saucepan.

5. Repeat steps 3 and 4 two more times. Upon the second time leave the hot water in the syringe.

6. Place the syringe in a cool draft-free place, preferable in a clean zip-lock bag

7. Allow it to cool for several hours before proceeding to Procedure Two.

Procedure Two: Transferring print spores into syringe

1. First clean your work area. This may involve wiping down all work surfaces with a diluted bleach solution and spraying the area liberally with a disinfectant such as Lysol.

2. Place the following materials in your glovebox or on the oven door working surface: The shot glass, your cooled syringes, the bottle of alcohol, a paper towel, your print (still in zip-lock baggie) and the lighter or alcohol flame.

3. Wash hands with antibacterial soap before proceeding further.

4. Fold the paper towel up into ? sections and soak a corner of it with the alcohol.

5. With the alcohol soaked towel wipe the interior of the shot glass, essentially sterilizing the surface you are about to use in the transfer. Allow the shot glass to air dry, should only take a few seconds.

6. Remove the needle guard from your sterile syringe and flame sterilize the needle. Then take your alcohol soaked paper towel and wipe the needle with it to further aid in the sterilization. At this point try to avoid letting the needle touch any other surface unless otherwise instructed to do so.

7. NOTE: it is important at this point to work as quickly as possible to help combat the chances of contaminating molds and bacteria falling into your work area and thereby ruining your syringe.

8. Remove the print from its storage baggie. Unfold it to expose the spores. Lightly begin to scrape, using the needle of the syringe, a section of the print off into the shot glass. For a medium sized print it is usually adequate to scrape off a section no larger than 1/5 of the total print.

9. You will have a small noticeable collection of spores in the shot glass. Now expunge no more than half of the water from the syringe into the shot glass, lightly stirring the spores into the solution.

10. Suck the spore water solution into the syringe. You may need to expunge some more water into the shot glass and re-suck to help in capturing all the spores into the syringe.

11. Once you have the spore solution back into the syringe you should notice that the water inside has turned a darker shade of color and you may see small clusters of spores floating in the solution. This is good, you have completed the process.

12. Sterilize the needle again with the alcohol soaked paper towel, replace the needle guard and place the syringe back into your clean zip-lock bag.

13. Allow the syringe to sit for no less than 12 hours before using it in jar inoculation. This is extremely important, as the spores must be allowed to rehydrate before they can be introduced into the substrate material. Failure to allow this may result in slow or no germination



--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/24/00
Posts: 5,015
Re: amazon??? [Re: phrozendata]
    #403857 - 09/25/01 07:19 PM (15 years, 2 months ago)

It would be in your best interest not to rush ahead, think your doing things right, and find out you have a bunch of contaminated jars/syringes. Take the proper time to research what you are doing, work out kinks in your procedure, learn the ropes.



--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: amazon??? [Re: stoner]
    #403869 - 09/25/01 07:30 PM (15 years, 2 months ago)

Post Deleted a la Obscurity


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Flaming Spore Syring Needle Question... FreshCaps 4,283 5 08/10/05 08:41 PM
by agar
* ?? Flaming needle VS. Alcohol soaked cotton ball ?? thekeeper 1,935 1 10/28/06 08:47 AM
by RogerRabbit
* Still air Box - DO NOT CLEAN WITH ALCOHOL
( 1 2 all )
veda_sticks 4,112 27 10/06/14 01:43 AM
by Sanguin3
* Getting ready to make my first spore syring fromthemoon 6,570 10 06/29/07 10:49 PM
by wikedferret
* Using alcohol lamp in glovebox? Jerrico 1,647 8 10/10/06 07:11 PM
by Blutjager
* DZ's Inoculation Tek, WITHOUT GLOVEBOX! DZ 3,131 13 06/09/09 01:49 PM
by Lennyk
* Sterilizing Needle jellyfishS 740 4 10/10/05 10:09 PM
by foxracinfan567
* Sterilizing the syringe needle (What is acceptable?)
( 1 2 all )
Gogol 2,411 21 10/07/08 04:19 AM
by c1dh3d

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
500 topic views. 22 members, 80 guests and 9 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Lil Shop Of Spores
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.051 seconds spending 0.004 seconds on 14 queries.