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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Monistat, Rye Grain

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Offline777D777
member
Registered: 08/19/01
Posts: 182
Loc: The Universe
Last seen: 13 years, 10 months
Gas exchange for jars?
    #403782 - 09/25/01 05:59 PM (15 years, 2 months ago)

Exactly how much oxygen does the mycelium need to grow? My gerbil inoculated 8 days ago and after 3 days they germinated but haven't grown much if at all since. They have been incubating in my closet under a heating mat that keeps it around 85-90 degrees. Maybe it is like the saying: A watched pot never boils. But i am worried cuz they haven't had significant growth in 5 days! Thanx

_______

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0----> sporeman is too! <----0
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Invisibleoscill8
*bondage fairy*
Registered: 02/27/01
Posts: 1,215
Re: Gas exchange for jars? [Re: 777D777]
    #404188 - 09/25/01 11:36 PM (15 years, 2 months ago)

incubation chamber like alien's http://alientek.iwarp.com is a place to lookat


--------------------
? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"


Edited by oscill8 (11/16/01 04:28 AM)


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Offlinetchyted
miestro
Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 2 years, 2 months
Re: Gas exchange for jars? [Re: 777D777]
    #404208 - 09/25/01 11:46 PM (15 years, 2 months ago)

zero. they don't really require oxygen. they may however get smothered by CO2

i should amend that zero. mycelium can germinate and grow in a sealed mason jar, that means no outside oxygen.

if your gerbil doesn't see significant change in a week or so, you probably have bacterial or yeast infection. sometimes this can be hospitalized by inverting the jar and loosening the lid, which tends to let CO2 out, and fresh air in, which in turn reduces moisture and may tend to slowly favor the mushrooms over the contams. same goes for filter disks. the objective is to get the CO2 out. if you have contams, they are likely producing huge amounts of CO2.

my cat has even gone as far as to temporarily remove the cake from the jar, and place it in a sterile terrarium, spraying it with 9 to 1 H2O2, in hopes of giving the mycelium a jump-start.

Edited by tchyted on 09/25/01 10:50 PM.



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Offlinedarkfly
LysergicExperimenter

Registered: 09/17/01
Posts: 1,169
Last seen: 1 year, 3 months
Re: Gas exchange for jars? [Re: tchyted]
    #404244 - 09/26/01 12:05 AM (15 years, 2 months ago)

oscill8, are you sure that is the correct website?

darkfly

-- too much trippin and my soul is worn thin


--------------------
Everything Is Blue In This World. The Deepest Shade Of Mushroom Blue.


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Invisibleoscill8
*bondage fairy*
Registered: 02/27/01
Posts: 1,215
Re: Gas exchange for jars? [Re: darkfly]
    #404357 - 09/26/01 01:19 AM (15 years, 2 months ago)

sorry, wrong address. damn, it must have moved, it was at http://alientek.iwarp.com, but its no longer there... argh. nice site too. damned acceptable use policies.

heres a link to the text only- alien's used to have great pics but... hey-
http://www.theforestfloor.org/archives/old_archive/aliens_cub_tek.html

Edited by oscill8 on 09/26/01 12:35 AM.



--------------------
? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"


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InvisibleZen Peddler
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Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: Gas exchange for jars? [Re: oscill8]
    #404468 - 09/26/01 03:59 AM (15 years, 2 months ago)

Good air exchange ensures rapid colonisation - otherwise the buildup of CO2 will stall the mycelium growth - take the tape off the holes and cover them with an alcohol swab which act as great filters...



www.shaman-australis.com/shroom/


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Offlinezhukov
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Registered: 12/21/00
Posts: 202
Loc: Australia
Last seen: 14 years, 7 months
Re: Gas exchange for jars? [Re: Zen Peddler]
    #404601 - 09/26/01 10:03 AM (15 years, 2 months ago)

Now that's a novel idea bm - alcohol swabs should work well! My own preference is to not use the lids, but to cover with foil instead with the ring carefully screwed on over a foil layer.
Then you innoculate thru the foil and cover the holes with micropore tape from a chemist which lets air in & should keep contams out.....



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OfflineDrAbeLincoln
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Registered: 08/31/01
Posts: 248
Last seen: 12 years, 6 months
Re: Gas exchange for jars? [Re: 777D777]
    #404613 - 09/26/01 10:18 AM (15 years, 2 months ago)

Get some filter discs..I just got some and I am having great results...I used dark rye flour instead of brown rice along with the filter discs and I've got about 75% colonization and its only been about 5 days



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OfflineCACA
veteran
Registered: 07/12/01
Posts: 1,122
Last seen: 14 years, 7 months
Re: Gas exchange for jars? [Re: DrAbeLincoln]
    #404616 - 09/26/01 10:21 AM (15 years, 2 months ago)

How does 3M Micropore tape compare to filter disks?

:frown: .. what was i saying..? Time for a cigarette.


--------------------
"I am the vine, ye are the branches: He that abideth in me, and I in him, the same bringeth forth much fruit: for without me ye can do nothing." John 15:5


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Offlinetort
member
Registered: 06/04/01
Posts: 101
Loc: AMERICA
Last seen: 15 years, 1 month
Re: Gas exchange for jars? [Re: DrAbeLincoln]
    #404639 - 09/26/01 10:52 AM (15 years, 2 months ago)

hey 7, where does your foaf get his/her filter disks and rye flour?

GOD BLESS AMERICA


--------------------
[red]GOD[/red] [white]BLESS[/white] [blue]AMERICA[/blue]


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OfflineXAZIA
glitter goddess
Registered: 08/22/01
Posts: 678
Loc: oklahoma
Last seen: 14 years, 2 months
Re: Gas exchange for jars? [Re: CACA]
    #404665 - 09/26/01 11:34 AM (15 years, 2 months ago)

you guys got smart pets!!

"Emancipate yourself from mental slavery, none but ourselves can free our minds."

Bob Marley



--------------------
"Emancipate yourself from mental slavery, none but ourselves can free our minds."

Bob Marley

Remember, EGOISM is the beginning, the source, and the root of EVIL!

http://www.fanaticus.com/pf-tek.htm
http://www.mycotopia.net/teks/hongus.html


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OfflineMAIA
World-BridgerKartikeya (DftS)
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Posts: 7,275
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Re: Gas exchange for jars? [Re: CACA]
    #404690 - 09/26/01 12:00 PM (15 years, 2 months ago)

I use waterproof banding aids with micro holes, works great and are cheap. My two cents.

Peace,
MAIA


"Houston, we have a problem... shrooms are over "
=)Free&Spread the Spores(=
Support the FSR


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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OfflineDrAbeLincoln
enthusiast

Registered: 08/31/01
Posts: 248
Last seen: 12 years, 6 months
Re: Gas exchange for jars? [Re: tort]
    #404997 - 09/26/01 05:06 PM (15 years, 2 months ago)

You can get the filter discs at micronmagick.com and the rye flour at any grocery store...the kind of I get just says organic dark rye flour on the bag...



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OfflineDaydreamer
journeyman
Registered: 09/07/01
Posts: 57
Loc: Vermont
Last seen: 14 years, 8 months
identical problem [Re: tchyted]
    #405743 - 09/27/01 02:46 AM (15 years, 2 months ago)

i just had the same problem. 7jars started growing, then stopped, the other 5 never started. i took the tape off the holes and inverted the jars in a sterile tupperware container, and the growth in the 7 jars took off, and within a day , there was significant growth in the other 5. (i should add that the state of the jars didn't change for more than a week prior.)

You took to much man, you took too much... too much...


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Monistat, Rye Grain

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