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Amazon Shop: ½ Pint Jars, Hot Plate, Pressure Cooker, Rye Grain

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OfflineMiller
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Registered: 11/28/04
Posts: 69
Last seen: 13 years, 21 days
Best way to steralize syringes
    #4034219 - 04/09/05 02:26 PM (13 years, 5 months ago)

I have a quart of liquid culture that is ready to be used. i have a dozen empty syringes that i'm going to use. whats the best way to steralize them?

i'm thinking fill with water and put in the pressure cooker. will that work. will they melt?


--------------------
~miller


What is the Scratchy?


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InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
Loc: One Inch ↑
Re: Best way to steralize syringes [Re: Miller]
    #4034226 - 04/09/05 02:30 PM (13 years, 5 months ago)

With LC, I've always just boiled some distilled water and fill up the syringe and spray it out in the sink.  I do this about 5-6 times, then let the last one sit in there and cool off.  Then I squirt most of it out, and draw in the LC... Haven't had a single jar contam... GL bro :stoned:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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OfflineMiller
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Registered: 11/28/04
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Last seen: 13 years, 21 days
Re: Best way to steralize syringes [Re: NeedMoreSleep]
    #4034242 - 04/09/05 02:34 PM (13 years, 5 months ago)

alright i'll give that a try.

how much LC do you use to inoculate? also what do you use to make your LC. This is my first shot at it, i did a jar with honey and one with corn starch, about a tablespoon of each. and the corn starch is looking a lot better than the honey. honey is barley showing anything


--------------------
~miller


What is the Scratchy?


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InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
Loc: One Inch ↑
Re: Best way to steralize syringes [Re: Miller]
    #4034252 - 04/09/05 02:40 PM (13 years, 5 months ago)

I do my LC in quart jars about halfway filled up w/ a tablespoon of light corn syrup.  I like to get a good thick amount of mycelium in my syringe and I use about 5cc's on quarts of rye.  I finally figured out the right water content for this amount of LC..... Plus I use a big syringe (60cc) :smile:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


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OfflineHotnuts
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Registered: 02/26/05
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Re: Best way to steralize syringes [Re: NeedMoreSleep]
    #4034301 - 04/09/05 03:00 PM (13 years, 5 months ago)

Wrap the syringes in aluminum foil and pc them for 30 minutes @ 15 psi.


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Amazon Shop: ½ Pint Jars, Hot Plate, Pressure Cooker, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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