Home | Community | Message Board


The Spore Depot
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Pressure Cooker, Rye Grain

Jump to first unread post. Pages: 1
OfflineMiller
Drunk

Registered: 11/28/04
Posts: 69
Last seen: 11 years, 7 months
Best way to steralize syringes
    #4034219 - 04/09/05 02:26 PM (12 years, 23 days ago)

I have a quart of liquid culture that is ready to be used. i have a dozen empty syringes that i'm going to use. whats the best way to steralize them?

i'm thinking fill with water and put in the pressure cooker. will that work. will they melt?


--------------------
~miller


What is the Scratchy?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
Loc: One Inch ↑
Re: Best way to steralize syringes [Re: Miller]
    #4034226 - 04/09/05 02:30 PM (12 years, 23 days ago)

With LC, I've always just boiled some distilled water and fill up the syringe and spray it out in the sink.  I do this about 5-6 times, then let the last one sit in there and cool off.  Then I squirt most of it out, and draw in the LC... Haven't had a single jar contam... GL bro :stoned:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMiller
Drunk

Registered: 11/28/04
Posts: 69
Last seen: 11 years, 7 months
Re: Best way to steralize syringes [Re: NeedMoreSleep]
    #4034242 - 04/09/05 02:34 PM (12 years, 23 days ago)

alright i'll give that a try.

how much LC do you use to inoculate? also what do you use to make your LC. This is my first shot at it, i did a jar with honey and one with corn starch, about a tablespoon of each. and the corn starch is looking a lot better than the honey. honey is barley showing anything


--------------------
~miller


What is the Scratchy?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleNeedMoreSleep
OCD = 100%Sterility :-)

Registered: 01/25/05
Posts: 1,210
Loc: One Inch ↑
Re: Best way to steralize syringes [Re: Miller]
    #4034252 - 04/09/05 02:40 PM (12 years, 23 days ago)

I do my LC in quart jars about halfway filled up w/ a tablespoon of light corn syrup.  I like to get a good thick amount of mycelium in my syringe and I use about 5cc's on quarts of rye.  I finally figured out the right water content for this amount of LC..... Plus I use a big syringe (60cc) :smile:


--------------------

"Now, we must all fear evil men. But there is another kind of evil,
which we must fear most. And that is... the indifference of good men."
-Boondock Saints


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHotnuts
old hand
 User Gallery

Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 10 months, 3 days
Re: Best way to steralize syringes [Re: NeedMoreSleep]
    #4034301 - 04/09/05 03:00 PM (12 years, 23 days ago)

Wrap the syringes in aluminum foil and pc them for 30 minutes @ 15 psi.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Pressure Cooker, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Corn Starch Liquid Mycelium Culture AlienPrimate 3,166 3 08/18/03 04:57 PM
by AlienPrimate
* Syringe Steralization jtk 871 14 06/16/05 04:55 AM
by blackout
* steralizing casing soil.....a little help please exoduc 10,184 17 04/12/07 02:09 AM
by FriendlyNeighbor
* Ooops... steralized my spawn substrate NStoney 1,102 15 07/02/09 08:46 PM
by prismism
* potato starch for agar? artizen 3,350 5 01/29/09 08:21 PM
by CheeWiz
* Steralizing verm for roll. Dr. Greenthumb 720 9 05/19/04 05:43 AM
by hawksapprentice
* I am scared to do a Print/syring! fearforce7 4,389 18 02/24/17 09:11 PM
by mushpunx
* steralization with a microwave rico357 1,172 11 12/20/02 11:45 PM
by kykeon

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
656 topic views. 35 members, 116 guests and 39 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Gaiana.nl
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.001 seconds on 14 queries.