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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Perlite, Vermiculite

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Offlinepunkgod94
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what did I do wrong?
    #4031221 - 04/08/05 04:58 PM (11 years, 7 months ago)

Ok, I tried growing twice so far and have been unsuccessful both times. First time I experienced several difficulties - some contamination and most of the jars didnt take the spores at all. Second time around things went good! 6 out of 6 jars all began to grow the mycelium. After a few weeks they slowed down and stopped growing, leaving the jars not fully colonized. I'd say they were only 50-75% colonized.

I heard a few recommendations to just cut away teh uncolonized portion and try to fruit anyway but I wanted to see if I could get them to fully colonize instead. Now months later theyre STILL not fully colonized. What could've stopped them from colonizing? I dont want to make the same mistake third time around.

The strain I was working with 2nd time was B+. I was using the BRF/verm/water method. I've also been told that the vermiculite I'm working with is very fine, but it's all I'm able to find in the area. I used a fishtank heater to keep their temperature up, but they still only managed to stay around 75-80 degrees with the tub in tub method.


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OfflinekronnyQ
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Re: what did I do wrong? [Re: punkgod94]
    #4031226 - 04/08/05 05:00 PM (11 years, 7 months ago)

Are you using larger jars? PF Tek does not work well with larger jars, they never make it to full colonization mostly from lack of fresh air.


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Offlinepunkgod94
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Re: what did I do wrong? [Re: kronnyQ]
    #4031233 - 04/08/05 05:03 PM (11 years, 7 months ago)

No, I was using 1/2 pint jars with 4 holes in the top and the tinfoil over top of them to keep contamination out. I made sure to not keep the foil completely covering the top though so some air exchange could occur.


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Offlinelordoftheshroomz
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Re: what did I do wrong? [Re: kronnyQ]
    #4031238 - 04/08/05 05:04 PM (11 years, 7 months ago)

yea if there is tape over the inoculation holes then take it off! let them breath!!!!
also a possibility is that ure substrate may be dried out in which case u should dunk them. you might have to do both of these things.

a tip for preventing substrate from drying out is to put perlite,water and h2o2 in the bottom of ure incubator and this will humidify things so any lost water is replaced!


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Offlinepunkgod94
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Re: what did I do wrong? [Re: lordoftheshroomz]
    #4031255 - 04/08/05 05:08 PM (11 years, 7 months ago)

No tape over the holes, just the loose tinfoil.

How exactly do you go about dunking them?

My incubator is just a tub in tub with the fishtank heater heating the water. Would I put the perlite and h2o2 in the inner tub or just in with the water in the outer tub?

Sorry if these are stupid questions, Im still a little high.


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OfflinekronnyQ
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Re: what did I do wrong? [Re: lordoftheshroomz]
    #4031265 - 04/08/05 05:09 PM (11 years, 7 months ago)

Also might want to consider Tyvek tops next time, allows for superb air exchange.

Perlite in an incubation chamber, I like it lord! I still have half a bag sitting around not being used.


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OfflinePowerTrip
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Re: what did I do wrong? [Re: punkgod94]
    #4031432 - 04/08/05 05:37 PM (11 years, 7 months ago)

My best guess is that your vermiculite isn't holding enough water for the jars to fully colonize. You should look around and try to find some better vermiculite, or even order some online if you have to. Vermiculite doesn't weigh much so it shouldn't be expensive to ship.

If you have four holes in the lid, with one piece of aluminum foil covering it, your air exchange should be fine. I'm using that same method and I have jars that are fully colonized in 12 days, and I really press that foil on tight. If you used tyvek instead I think it would only add to your dehydration problem. Do the jars look dry by the way?


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OfflineWoland
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Re: what did I do wrong? [Re: PowerTrip]
    #4031505 - 04/08/05 05:57 PM (11 years, 7 months ago)

I'd take those jars and dunk em'. Then put them in a plastic bag. Crumble them a little to mix together, then let it colonize. Like a Cpr for cakes.


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Offlinepunkgod94
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Re: what did I do wrong? [Re: PowerTrip]
    #4031507 - 04/08/05 05:57 PM (11 years, 7 months ago)

Well right now theyre bone dry because its been a couple months, but from what I recall they appeared to be a LITTLE dry. I think I made a post asking if it was ok to just add more water to the brf/verm/water mixture while mixing the substrate but I was told that was a bad idea and to stick to the regular ratio. Someone mentioned that I could sterilize some water in syringes and inject that but that seemed like way too much work and opened the possibility for contaminants to get in so I bagged that idea.


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Offlinepunkgod94
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Re: what did I do wrong? [Re: punkgod94]
    #4031526 - 04/08/05 06:03 PM (11 years, 7 months ago)

Sorry to be annoying, but where on the shroomery can I find out how to dunk? I can't find the link. Is casing the same thing as dunking?

Edit: Whoops, found the dunking info and it's not the same as casing. Forget my question. I dont think it would be a good idea to dunk the jars now though cause theyre months old. And in addition it looks like theyre turning yellowish. I dont know if that color means theyre trying to pin in the jar or not, but it doesnt look like mold or any other conteminant. Ideas?


Edited by punkgod94 (04/08/05 06:23 PM)


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OfflineUnderhillmaster
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Re: what did I do wrong? [Re: punkgod94]
    #4032728 - 04/09/05 12:19 AM (11 years, 7 months ago)

Did you flip your jars over? I usually flip my jars at about day 10 for a gas exchange boost. And I think you should feel free to fine tune your water ratio in your brf/verm mix. I don't know if it is because of my altitude or humidity, or even humidity in my incubator, but I have to add a little extra water on my batches. I usually have to use 6cups verm/3cups brf/3.5 cups water.


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OfflineHippieChick
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Re: what did I do wrong? [Re: punkgod94]
    #4032762 - 04/09/05 12:36 AM (11 years, 7 months ago)

Yes, the yellow is more then likely mycelium waste or piss for lack of a better word. After 2 months this is quite normal. If you think they're almost a lost cause, you could try birthing, cutting out uncolonized part, dunking, rolling and double end casing and see what happens. Good Luck.
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Edited by HippieChick (04/09/05 12:37 AM)


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Offlinepunkgod94
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Re: what did I do wrong? [Re: HippieChick]
    #4033936 - 04/09/05 12:40 PM (11 years, 7 months ago)

Yea, I flipped the jars over. It was after more than 10 days though so I'll try to flip them a little sooner next time. And I just may add a tad more water as well. Thanks for the suggestion HippieChick, but I think these jars are really a lost cause. Even if they DID somehow manage to work out it seems like a lot of work and I just wouldn't feel right partaking in the fruits of the labor because I've already ignored them for so many months. I'm gonna try starting fresh with all the new suggestions I've received. Thanks for your input though :smile:


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OfflineHippieChick
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Re: what did I do wrong? [Re: punkgod94]
    #4034105 - 04/09/05 01:52 PM (11 years, 7 months ago)

Don't forget. Nothing in this hobby is a lost cause if you can gain some knowledge or experience from it.
Peace,Love,Happiness and Harmony.
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--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Perlite, Vermiculite

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