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Offlinejtk
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Center Inoculation
    #4028921 - 04/08/05 12:22 AM (11 years, 7 months ago)

If you inoculate your jars in the center straight in will they colonize from the center towards the outside?

I just did some WBS jars w/ 1 hole right in the center. I didnt angle the needle at all towards the sides. I was just wondering if myc. can grow from the center to the outside if inoculated this way?


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Offlinedr0mni
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Re: Center Inoculation [Re: jtk]
    #4028937 - 04/08/05 12:26 AM (11 years, 7 months ago)

yes

plain and simple


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Offlinejtk
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Re: Center Inoculation [Re: dr0mni]
    #4028961 - 04/08/05 12:32 AM (11 years, 7 months ago)

I thought it was probably possible, but i see hardly any visible myc. on the bottom center, and none at the top center. So either there's colonization coming from the very center, or it's just on the edges of my jars. Because theres spots all over the outside edges of my jars, but they are little and real random. That's why i think it's coming from the center, because i didn't inoculate them at all on the outside.


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Edited by jtk (04/08/05 12:39 AM)


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Re: Center Inoculation [Re: jtk]
    #4028988 - 04/08/05 12:41 AM (11 years, 7 months ago)

inoculate wbs jars through 1 center hole and then shake up when you've covered the wole...this way the spores get more distributed.


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Offlinejtk
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Re: Center Inoculation [Re: TODAY]
    #4028991 - 04/08/05 12:41 AM (11 years, 7 months ago)

i guess i did shake them a little after inoculation

ive also shaken them 1 time since inoculation, when they were at about 5%.


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Re: Center Inoculation [Re: jtk]
    #4028998 - 04/08/05 12:44 AM (11 years, 7 months ago)

are you incubating them and did you take precaution to remain sterile? If yes then dont worry you'll be fine. If not i dont know what to say.


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Re: Center Inoculation [Re: xburn]
    #4029008 - 04/08/05 12:46 AM (11 years, 7 months ago)

We'll for the first 1.5 weeks i had them at 70. Now for the last 4 or so days they've been incubated at 80-82. I did take precaution to remain sterile, and they all seem to be contam free.


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Re: Center Inoculation [Re: jtk]
    #4029027 - 04/08/05 12:52 AM (11 years, 7 months ago)

ok
do you have a TiT?
thats what i am using it cost less than 30 doallars to set up and works great.
70 is to cold and is probably why your not seeing much growth. I would pretend you just inoculated those jars 7 days ago and expect growth like that if you now running 80-82 constant


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Edited by xburn (04/08/05 12:53 AM)


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Offlinejtk
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Re: Center Inoculation [Re: xburn]
    #4029034 - 04/08/05 12:54 AM (11 years, 7 months ago)

i dont have a TiT. I have a heat bomb set up right now that keeps my incubation chamber at a nice constant 80-82.

Should i shake my jars again, because i haven't shaken them at all since being at the 80-82?


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Re: Center Inoculation [Re: xburn]
    #4029049 - 04/08/05 12:59 AM (11 years, 7 months ago)

yeah shaking every now and again cant hurt just spread the mycellium

is your grain drying out after 2 weeks?


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Re: Center Inoculation [Re: xburn]
    #4029053 - 04/08/05 01:00 AM (11 years, 7 months ago)

someone said they could be, but there is a decent amount of condensation accumlating in the jars since i put them at 80-82, so i think there is still a sufficient amount of water in there.


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Amazon Shop for: ½ Pint Jars, Wild Bird Seed

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