Home | Community | Message Board


Marijuana Demystified
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Malt Extract

Jump to first unread post. Pages: 1
OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 7 months
A Liquid Culture Question
    #4027254 - 04/07/05 06:19 PM (12 years, 7 months ago)

A FOAF has made a number of attempts at liquid culture jars, procedure as follows:

1 Tbsp Light Malt Extract
1 Tbsp Dextrose
...are both slowly mixed in with about 2/3 of a quart of boiling distilled water, and allowed to boil for 10-15 min. Resulting liquid is strained 3 times through coffee filters, poured into a quart sized canning jar, *EDIT* PC'd at 15 psi for 20-30 min, *EDIT* and allowed to cool overnight before injection of spores.

On every occasion, said FOAF wakes up in the morning to find a light brown, powdery (malt-looking) substance resting at the bottom of the jar. He has been reluctant to innoc these jars, as he has seen many pictures of liquid culture projects without this powder at the bottom. Is he using too much malt? Should he leave out the dextrose? On his most recent attempt, he strained the hot mixture 6 times before allowing it to cool, and he was left with similar results. He has tried lids w/gas exchange as well as airtight with no variation. Any advice?


--------------------


Edited by LengthyFellow (04/07/05 06:50 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleGlassOnion
Stranger
Registered: 06/23/04
Posts: 282
Loc: Antartica
Re: A Liquid Culture Question [Re: LengthyFellow]
    #4027298 - 04/07/05 06:27 PM (12 years, 7 months ago)

You have to pressure cook the liquid culture solution..not just boil it.
And you would be better off only filling the quart jars only 1/3. Adding a few pieces of glass or marble can aid in breaking up the mycelium.


Edited by GlassOnion (04/07/05 06:27 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegoofy98
Heart of ahustler wrist ofa chemist
 User Gallery

Registered: 03/18/05
Posts: 451
Loc: next door
Last seen: 11 years, 7 months
Re: A Liquid Culture Question [Re: GlassOnion]
    #4027333 - 04/07/05 06:32 PM (12 years, 7 months ago)

strain when cooled and then RE-PC. the stuff at the bottom probsbly percipitate when the solution cools


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 7 months
Re: A Liquid Culture Question [Re: GlassOnion]
    #4027335 - 04/07/05 06:33 PM (12 years, 7 months ago)

*Edit* Unnecissary post


--------------------


Edited by LengthyFellow (04/07/05 06:50 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 7 months
Re: A Liquid Culture Question [Re: goofy98]
    #4027340 - 04/07/05 06:33 PM (12 years, 7 months ago)

Quote:

goofy98 said:
strain when cooled and then RE-PC. the stuff at the bottom probsbly percipitate when the solution cools




He will try this today


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegoofy98
Heart of ahustler wrist ofa chemist
 User Gallery

Registered: 03/18/05
Posts: 451
Loc: next door
Last seen: 11 years, 7 months
Re: A Liquid Culture Question [Re: LengthyFellow]
    #4027344 - 04/07/05 06:34 PM (12 years, 7 months ago)

nice vapor brother by the way


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 7 months
Re: A Liquid Culture Question [Re: goofy98]
    #4027384 - 04/07/05 06:42 PM (12 years, 7 months ago)

Why thank you....i think its technically a VaporDoc but its all the same. I used to have a nicer whip (with the glass on glass connector) but it broke :frown:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletahoe
Noob Slayer
Male

Registered: 11/27/03
Posts: 6,230
Loc: N38.93829W119.98108
Trusted Cultivator
Re: A Liquid Culture Question [Re: LengthyFellow]
    #4027396 - 04/07/05 06:44 PM (12 years, 7 months ago)

Quote:

LengthyFellow said:
A FOAF has made a number of attempts at liquid culture jars, procedure as follows:

1 Tbsp Light Malt Extract
1 Tbsp Dextrose
...are both slowly mixed in with about 2/3 of a quart of boiling distilled water, and allowed to boil for 10-15 min. Resulting liquid is strained 3 times through coffee filters, poured into a quart sized canning jar and allowed to cool overnight before injection of spores.

On every occasion, said FOAF wakes up in the morning to find a light brown, powdery (malt-looking) substance resting at the bottom of the jar. He has been reluctant to innoc these jars, as he has seen many pictures of liquid culture projects without this powder at the bottom. Is he using too much malt? Should he leave out the dextrose? On his most recent attempt, he strained the hot mixture 6 times before allowing it to cool, and he was left with similar results. He has tried lids w/gas exchange as well as airtight with no variation. Any advice?



your tek screams contamination!!! No need to strain the dextrose, no need for distiled water, too much sugar. Use either dextrose or malt but not both. 1tbsp is way too much for 2/3 quart of water let alone 2 tbsp. Just use 1 tsp of dextrose for that 2/3rds quart of water, dont strain.
This is dextrose that isnt strained. The cloudiness is mycelium


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 7 months
Re: A Liquid Culture Question [Re: LengthyFellow]
    #4027397 - 04/07/05 06:44 PM (12 years, 7 months ago)

My personal favorite is the Jerome Baker TB-04 to the left...a CLASSIC!


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinegoofy98
Heart of ahustler wrist ofa chemist
 User Gallery

Registered: 03/18/05
Posts: 451
Loc: next door
Last seen: 11 years, 7 months
Re: A Liquid Culture Question [Re: LengthyFellow]
    #4027410 - 04/07/05 06:47 PM (12 years, 7 months ago)

i usually have to strain with malt or honey. Dextrose is cleaner i agree. Stupid sugars carmalize when using honey or malt.
Cops got my JB couple years ago.... so sad.
I love the hands free whip though for the VD. im so lazy


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 7 months
Re: A Liquid Culture Question [Re: tahoe]
    #4027414 - 04/07/05 06:48 PM (12 years, 7 months ago)

Quote:

tahoe said:

Just use 1 tsp of dextrose for that 2/3rds quart of water, dont strain.





Cool, thanks


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Malt Extract

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* liquid culture question ??
( 1 2 3 all )
niall_b73 4,667 44 04/01/10 08:10 PM
by Impatient
* Liquid Culture Questions? Shroomer Smurf 1,813 9 11/14/08 09:57 PM
by Shroomer Smurf
* . *DELETED* *DELETED* wisp 2,067 15 04/22/08 08:32 AM
by Fahkface
* liquid culture question
btronson
1,056 8 01/24/09 03:48 PM
by Spongiform
* 2 liquid culture questions: spliffmasta 877 3 11/29/04 02:42 PM
by Koala Koolio
* Agar vs. Liquid Culture Question KauaiOrca 2,998 16 08/15/08 12:29 AM
by just me
* Liquid culture question spectrem12 882 4 04/25/07 11:19 PM
by Quake3
* liquid culture question stewy1412 1,053 16 09/06/08 07:41 AM
by Steve

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,750 topic views. 18 members, 59 guests and 26 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Everything Mushrooms
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.006 seconds on 19 queries.