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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflineNicerOo
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stalled colonization of jar?
    #4019613 - 04/06/05 12:50 AM (13 years, 1 month ago)

The colonization of a jar seems to have stopped for over a week. It is not infected. It hasn't changed color or appearance or anything. Certain parts of the outside of the jar just aren't being taken over with mycellium. I think it might be because there is not enough moisture in the jar...is this a possibility? What course of action should be taken? Should more water be injected into the partially colonized jar?


Edited by NicerOo (04/06/05 12:51 AM)


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OfflineGNIOM1498
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Re: stalled colonization of jar? [Re: NicerOo]
    #4019623 - 04/06/05 12:55 AM (13 years, 1 month ago)

brf? im guessing then u could add sterile water to it but i wouldn't i tried it before just makes puddle. i would just cut the off whats not colonized.


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InvisibleCrasher
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Re: stalled colonization of jar? [Re: GNIOM1498]
    #4019662 - 04/06/05 01:07 AM (13 years, 1 month ago)

What temp, what substrate? How long was it colonizing before the stall? Does it smell funny?


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OfflineNicerOo
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Re: stalled colonization of jar? [Re: Crasher]
    #4019823 - 04/06/05 01:54 AM (13 years, 1 month ago)

total darkness in the mid-high 70s...

this is for the formula reccomended in the newbie guide...
for 1/2 pint canning jars (250 ml):

1/4 cup of brown rice powder
1/2 cup of vermiculite
45 - 60 ml (cc) water (1/4 cup = 59 ml)


it colonized for maybe 2 weeks before stalling...

it doesn't smell funny through the masking tape; risk of infection has kept the jar closed

should it be placed in indirect light?


Edited by NicerOo (04/06/05 01:54 AM)


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Invisibleactionshroom
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stalled colonization of jar? [Re: NicerOo]
    #4019835 - 04/06/05 01:56 AM (13 years, 1 month ago)

Take the tape off to allow oxygen into the jar.


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OfflineNicerOo
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Re: stalled colonization of jar? [Re: NicerOo]
    #4019842 - 04/06/05 01:58 AM (13 years, 1 month ago)

^^that won't contaminate it?

also, the jar was turned upside down after it stalled for a bit


Edited by NicerOo (04/06/05 01:59 AM)


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Invisibleactionshroom
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stalled colonization of jar? [Re: NicerOo]
    #4019860 - 04/06/05 02:03 AM (13 years, 1 month ago)

It's very unlikely it will contaminate. The vermiculite is a barrier for contaminants. In the past I have fully opened jars in non-sterile environments without contamination.


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OfflineMindzpore
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Re: stalled colonization of jar? [Re: actionshroom]
    #4020478 - 04/06/05 07:16 AM (13 years, 1 month ago)

i suppose it depends on how dirty your place really is and contaminant fallout at the place you are storing for colonization.

i've done the same as actionshroom and left jars without lid and also i almost never bothered with tape. contamination was very unusual (enough to surprise me whenever it happened). but then again... my place was relatively clean, i did all procedures in a glove box and my colonizations spot was very clean with no (very little) moving air.


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Re: stalled colonization of jar? [Re: NicerOo]
    #4020518 - 04/06/05 07:56 AM (13 years, 1 month ago)

Pull the tape off...the verm barrier will protect the substrate.

The tape is what is stalling your jar...you have a CO2 build up.

The jar will take off again after you pull the tape off.

good luck!~


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OfflineNicerOo
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Re: stalled colonization of jar? [Re: actionshroom]
    #4020687 - 04/06/05 10:18 AM (13 years, 1 month ago)

how long should the tape come off for? until its ready for birthing?


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InvisibleRoadkillM
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Re: stalled colonization of jar? [Re: NicerOo]
    #4020771 - 04/06/05 10:46 AM (13 years, 1 month ago)

Quote:

NicerOo said:

how long should the tape come off for? until its ready for birthing?




Yep

your done with the tape now.

TC


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinejamsandwich
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Re: stalled colonization of jar? [Re: Roadkill]
    #4021364 - 04/06/05 01:29 PM (13 years, 1 month ago)

what is the point of having tape then?


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InvisiblePawPaw
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Re: stalled colonization of jar? [Re: jamsandwich]
    #4021375 - 04/06/05 01:33 PM (13 years, 1 month ago)

Quote:

jamsandwich said:
what is the point of having tape then?



So you can tape stuff ...


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InvisibleRoadkillM
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Re: stalled colonization of jar? [Re: jamsandwich]
    #4021387 - 04/06/05 01:38 PM (13 years, 1 month ago)

Quote:

jamsandwich said:

what is the point of having tape then?




to keep the water out during sterilization.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinejamsandwich
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Re: stalled colonization of jar? [Re: Roadkill]
    #4021409 - 04/06/05 01:44 PM (13 years, 1 month ago)

i'm so glad i found this forum. its been 6 days and my jars have been taped the whole time. there is myc growth in 6 of the 8 jars, but now i'm hoping they will take off.


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InvisibleRoadkillM
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Re: stalled colonization of jar? [Re: jamsandwich]
    #4021417 - 04/06/05 01:46 PM (13 years, 1 month ago)

I pull the tape off my BRF/Verm jars when the mycelium is about the size of a Quarter or 50 cent pc. inside the jars.

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinejamsandwich
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Re: stalled colonization of jar? [Re: Roadkill]
    #4021429 - 04/06/05 01:48 PM (13 years, 1 month ago)

of the 6 that have myc, they are all about the size of a quarter. I'm hoping for the other 2 jars, but something may have gone wrong.


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8 years now! lol


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InvisibleRoadkillM
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Re: stalled colonization of jar? [Re: jamsandwich]
    #4021450 - 04/06/05 01:53 PM (13 years, 1 month ago)

how many days has it been since you inoculated your jars?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineNicerOo
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Re: stalled colonization of jar? [Re: Roadkill]
    #4093842 - 04/25/05 01:58 AM (13 years, 1 month ago)

ok...it's been about 20 days since i took off the tape...
about 12 days ago i injected some water into the cake because taking off the tape did nothing...
i'm still really not seeing much of anything...

is my only option taking off the lid? do you really think this is what's holding it back? i'm very baffled o.O =(


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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