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Tweexican
Clit Commander
Registered: 11/06/03
Posts: 657
Last seen: 5 years, 8 months
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liquid culture
#4011598 - 04/04/05 02:44 AM (18 years, 11 months ago) |
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for those of you who propogate mycelium in a liquid culture and use quart jars... how do you retrieve the mycelium once it's ready to be taken into a syringe???
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 28 days
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Re: liquid culture [Re: Tweexican]
#4011604 - 04/04/05 02:46 AM (18 years, 11 months ago) |
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i shake it up and suck it up with a syringe.
if the myc won't break up, a sterilized marble or something around that size does the trick.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut "it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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Tweexican
Clit Commander
Registered: 11/06/03
Posts: 657
Last seen: 5 years, 8 months
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Re: liquid culture [Re: ZeroArmy27]
#4011611 - 04/04/05 02:48 AM (18 years, 11 months ago) |
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zero, do you use quart jars? i've only ever used pint jars for liquid myc. I see all these pictures of quar jars but im afraid that i wont be able to get my needle all the way into the jar without causing contamination. any ideas?
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rocknliam
happy willowdweller
Registered: 12/12/03
Posts: 307
Last seen: 10 years, 3 months
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Re: liquid culture [Re: Tweexican]
#4011625 - 04/04/05 02:53 AM (18 years, 11 months ago) |
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I did my liquid culture in a tupperware container, i realised when it came time to fill up the syringe i wouldnt be able to reach the liquid. So i took the container into a clean environment and opened the lid completely and sucked it up like that. I am very unsterile and never seem to have problems, i guess i have been lucky.
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Hotnuts
old hand
Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 2 months, 22 days
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Re: liquid culture [Re: rocknliam]
#4011647 - 04/04/05 03:08 AM (18 years, 11 months ago) |
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Gently swirl for a couple minutes everday to ensure that the tissue will be managable for aspiration!
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Re: liquid culture [Re: Tweexican]
#4011659 - 04/04/05 03:25 AM (18 years, 11 months ago) |
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I drill a hole close to the bottom of the quart jar and plug it with an injection plug. Works beautifully!
Here's a couple pics
I used a 20 dollar dremel drill from wall mart with some diamond bits. Takes a while. Have to run it on 5,000-10,000 rpm(the only settings) to avoid overheating and breaking the glass. Holding the jar under a stream of room temp water while drilling is a good idea too.
Throw in a couple pieces of broken glass, marbles whatever before sterilization.
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Here's the el cheapo dremel
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bixo
Dremel Addict
Registered: 02/14/05
Posts: 388
Loc: The Autobahn
Last seen: 10 years, 11 months
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i recently started using baby bottles with the nips turned down and they work great
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid culture [Re: Tweexican]
#4011735 - 04/04/05 05:17 AM (18 years, 11 months ago) |
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Any lid that will PC, fit a quart jar, couple pieces of rubber tubing, drill, bit, polyfil & rtv high temp gasket sealer.
Or same with metal lid
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ohmatic
searcher
Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: liquid culture [Re: agar]
#4011741 - 04/04/05 05:24 AM (18 years, 11 months ago) |
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i cant hold back.
screw the fancy, do the simple!
simply throw some glass sheds in there, rotate for a min or two and draw up through self healing silicone injection port.
yadda yadda! peace ohm
-------------------- MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid culture [Re: ohmatic]
#4011780 - 04/04/05 05:59 AM (18 years, 11 months ago) |
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Tubing method is better. Why? You have tyvek under the lid, which you must poke needle hole in to suck out solution. Tyvek gets damp & will WICK in contams.
With tubing, you can clamp off & keep filter DRY.
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s3x3s
Stranger
Registered: 03/11/05
Posts: 40
Last seen: 18 years, 11 months
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Re: liquid culture [Re: agar]
#4011866 - 04/04/05 07:17 AM (18 years, 11 months ago) |
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Ok, I just have to ask the dumbest question... You guys are getting all fancy with it which is way cool and all, but why not tip the damn jar enough to bring the liquid ( assuming you have reduced the contents to a homogeneous slurry ) close to the lid ( where the needle can reach ) and extract from there? s3x3s
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ohmatic
searcher
Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: liquid culture [Re: agar]
#4011971 - 04/04/05 08:20 AM (18 years, 11 months ago) |
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Quote:
agar said: Tubing method is better. Why? You have tyvek under the lid, which you must poke needle hole in to suck out solution. Tyvek gets damp & will WICK in contams.
With tubing, you can clamp off & keep filter DRY.
!!!
dint think of that, but actually i did couple of cultures with my method, did it three times to be precise and let them go for 2-3 weeks, no contams peace ohm
-------------------- MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid culture [Re: ohmatic]
#4011981 - 04/04/05 08:29 AM (18 years, 11 months ago) |
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LOL..... whatever works for you, DOES. One cannot complain about that. It's a good thing.
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Tweexican
Clit Commander
Registered: 11/06/03
Posts: 657
Last seen: 5 years, 8 months
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Re: liquid culture [Re: agar]
#4012475 - 04/04/05 11:54 AM (18 years, 11 months ago) |
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agar, i dont understand how the tubes come into play when pulling the culture out of the jar without introducing contams
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: liquid culture [Re: agar]
#4013741 - 04/04/05 06:07 PM (18 years, 11 months ago) |
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Quote:
agar said: Tubing method is better. Why? You have tyvek under the lid, which you must poke needle hole in to suck out solution. Tyvek gets damp & will WICK in contams.
With tubing, you can clamp off & keep filter DRY.
I agree with not using the tyvek, it doesnt seem to have any use in lc's. As for lids i havent tried agars tek but this works good for me. I never get the polyfill wet because i use small mouth jars here as Mr stamets recomends and i only fill them half way. I get about 150ml of lc which is plenty. i used to use 1/2 gallon jars but i just never got around to using all of it.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: liquid culture [Re: Tweexican]
#4013897 - 04/04/05 06:36 PM (18 years, 11 months ago) |
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Quote:
Tweexican said: agar, i dont understand how the tubes come into play when pulling the culture out of the jar without introducing contams
The pictures HERE don't explain it to you?
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PawPaw
Picktish TexasStyle....
Registered: 12/20/04
Posts: 8,243
Loc: was born down in the sout...
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Re: liquid culture [Re: tahoe]
#4014048 - 04/04/05 07:14 PM (18 years, 11 months ago) |
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Hey myth buster quit scaring our moderators They get their feelings hurt really easily
-------------------- Come to Paw Paws place there will be no sleeping tonight Eric just wants to catch reds .. Don?t look back, don?t look back He?s right on your trail Don?t look back, don?t look back He?s just a step away from hell WDYWFM?
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: liquid culture [Re: PawPaw]
#4014261 - 04/04/05 07:53 PM (18 years, 11 months ago) |
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i think agar and i are cool, there is only one that is still on my shit list.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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