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Amazon Shop: ½ Pint Jars, Agar, Paul Stamets

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OfflineTweexican
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liquid culture
    #4011598 - 04/04/05 04:44 AM (12 years, 2 months ago)

for those of you who propogate mycelium in a liquid culture and use quart jars... how do you retrieve the mycelium once it's ready to be taken into a syringe???


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OfflineZeroArmy27
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Re: liquid culture [Re: Tweexican]
    #4011604 - 04/04/05 04:46 AM (12 years, 2 months ago)

i shake it up and suck it up with a syringe.

if the myc won't break up, a sterilized marble or something around that size does the trick.


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OfflineTweexican
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Re: liquid culture [Re: ZeroArmy27]
    #4011611 - 04/04/05 04:48 AM (12 years, 2 months ago)

zero, do you use quart jars? i've only ever used pint jars for liquid myc. I see all these pictures of quar jars but im afraid that i wont be able to get my needle all the way into the jar without causing contamination. any ideas?


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Offlinerocknliam
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Re: liquid culture [Re: Tweexican]
    #4011625 - 04/04/05 04:53 AM (12 years, 2 months ago)

I did my liquid culture in a tupperware container, i realised when it came time to fill up the syringe i wouldnt be able to reach the liquid. So i took the container into a clean environment and opened the lid completely and sucked it up like that. I am very unsterile and never seem to have problems, i guess i have been lucky.


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OfflineHotnuts
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Re: liquid culture [Re: rocknliam]
    #4011647 - 04/04/05 05:08 AM (12 years, 2 months ago)



Gently swirl for a couple minutes everday to ensure that the tissue will be managable for aspiration!


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InvisibleMrMaddHatter
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Re: liquid culture [Re: Tweexican]
    #4011659 - 04/04/05 05:25 AM (12 years, 2 months ago)

I drill a hole close to the bottom of the quart jar and plug it with an injection plug. Works beautifully!

Here's a couple pics







I used a 20 dollar dremel drill from wall mart with some diamond bits.
Takes a while. Have to run it on 5,000-10,000 rpm(the only settings) to avoid overheating and breaking the glass. Holding the jar under a stream of room temp water while drilling is a good idea too.

Throw in a couple pieces of broken glass, marbles whatever before sterilization. :thumbup:


:cool:


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InvisibleMrMaddHatter
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Re: liquid culture [Re: MrMaddHatter]
    #4011676 - 04/04/05 05:50 AM (12 years, 2 months ago)

Here's the el cheapo dremel



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Offlinebixo
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Re: liquid culture [Re: MrMaddHatter]
    #4011680 - 04/04/05 05:57 AM (12 years, 2 months ago)

i recently started using baby bottles with the nips turned down and they work great


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Invisibleagar
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Re: liquid culture [Re: Tweexican]
    #4011735 - 04/04/05 07:17 AM (12 years, 2 months ago)

Any lid that will PC, fit a quart jar, couple pieces of rubber tubing, drill, bit, polyfil & rtv high temp gasket sealer.



Or same with metal lid


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Invisibleohmatic
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Re: liquid culture [Re: agar]
    #4011741 - 04/04/05 07:24 AM (12 years, 2 months ago)

i cant hold back.

screw the fancy, do the simple!



simply throw some glass sheds in there, rotate for a min or two and draw
up through self healing silicone injection port.

:laugh:

yadda yadda!
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibleagar
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Re: liquid culture [Re: ohmatic]
    #4011780 - 04/04/05 07:59 AM (12 years, 2 months ago)

Tubing method is better. Why? You have tyvek under the lid, which you must poke needle hole in to suck out solution. Tyvek gets damp & will WICK in contams.

With tubing, you can clamp off & keep filter DRY.


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Offlines3x3s
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Re: liquid culture [Re: agar]
    #4011866 - 04/04/05 09:17 AM (12 years, 2 months ago)

Ok, I just have to ask the dumbest question...
You guys are getting all fancy with it which is way cool and all, but why not tip the damn jar enough to bring the liquid ( assuming you have reduced the contents to a homogeneous slurry ) close to the lid ( where the needle can reach ) and extract from there?
s3x3s


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Invisibleohmatic
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Re: liquid culture [Re: agar]
    #4011971 - 04/04/05 10:20 AM (12 years, 2 months ago)

Quote:

agar said:
Tubing method is better. Why? You have tyvek under the lid, which you must poke needle hole in to suck out solution. Tyvek gets damp & will WICK in contams.

With tubing, you can clamp off & keep filter DRY.




!!!

dint think of that, but actually i did couple of cultures with my method,
did it three times to be precise and let them go for 2-3 weeks,
no contams :smile:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibleagar
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Re: liquid culture [Re: ohmatic]
    #4011981 - 04/04/05 10:29 AM (12 years, 2 months ago)

LOL..... whatever works for you, DOES. One cannot complain about that. It's a good thing. :thumbup: :wink:


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OfflineTweexican
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Re: liquid culture [Re: agar]
    #4012475 - 04/04/05 01:54 PM (12 years, 2 months ago)

agar, i dont understand how the tubes come into play when pulling the culture out of the jar without introducing contams


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Invisibletahoe
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Re: liquid culture [Re: agar]
    #4013741 - 04/04/05 08:07 PM (12 years, 2 months ago)

Quote:

agar said:
Tubing method is better. Why? You have tyvek under the lid, which you must poke needle hole in to suck out solution. Tyvek gets damp & will WICK in contams.

With tubing, you can clamp off & keep filter DRY.



I agree with not using the tyvek, it doesnt seem to have any use in lc's. As for lids i havent tried agars tek but this works good for me. I never get the polyfill wet because i use small mouth jars here as Mr stamets recomends and i only fill them half way. I get about 150ml of lc which is plenty. i used to use 1/2 gallon jars but i just never got around to using all of it.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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Invisibleagar
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Re: liquid culture [Re: Tweexican]
    #4013897 - 04/04/05 08:36 PM (12 years, 2 months ago)

Quote:

Tweexican said:
agar, i dont understand how the tubes come into play when pulling the culture out of the jar without introducing contams




The pictures HERE don't explain it to you?


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InvisiblePawPaw
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Registered: 12/20/04
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Re: liquid culture [Re: tahoe]
    #4014048 - 04/04/05 09:14 PM (12 years, 2 months ago)

Hey myth buster quit scaring our moderators :tongue2:
They get their feelings hurt really easily


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Invisibletahoe
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Re: liquid culture [Re: PawPaw]
    #4014261 - 04/04/05 09:53 PM (12 years, 2 months ago)

i think agar and i are cool, there is only one that is still on my shit list.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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Amazon Shop: ½ Pint Jars, Agar, Paul Stamets

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