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Offlinejtk
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WBS Moisture Content?
    #4010410 - 04/03/05 11:23 PM (16 years, 8 months ago)

I just did 4 qt. WBS jars using the 6T WBS Tek on this site: http://www.shroomery.org/index/par/23619. My jars started to colonize on the 5th day, but now on the 11-12 day they seem to have stalled at about 5%. They are incubating at 70-75 which i know is low, but everyone said it would be fine just take about 3-4 weeks to fully colonize. Well at the rate they're going it will never be fully colonized. Some said my moisture content could be too low, but i followed the tek exactly. I soaked 18 hours then drained 40 min. loaded jars 3/4 full and PC'd for 80min. I dont know what i did wrong?


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InvisibleHolydiver
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Re: WBS Moisture Content? [Re: jtk]
    #4010418 - 04/03/05 11:25 PM (16 years, 8 months ago)

Low temps are killing you. Anything dries out over time, and it sounds like your jars are in the process of it, or already have.

Build an incubator my friend, 70 isn't going to hack it.


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InvisibleBurke Dennings
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Re: WBS Moisture Content? [Re: jtk]
    #4010419 - 04/03/05 11:25 PM (16 years, 8 months ago)



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Offlinejtk
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Re: WBS Moisture Content? [Re: Burke Dennings]
    #4010436 - 04/03/05 11:27 PM (16 years, 8 months ago)

I talked to various ppl on this site that said they incubate at those temps and have fine results.


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InvisibleBurke Dennings
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Re: WBS Moisture Content? [Re: jtk]
    #4010448 - 04/03/05 11:30 PM (16 years, 8 months ago)

Well, it doesn't seem to be working out for you.  :shrug:

Seriously, try incubating.  What do you have to lose?  Just incubate at 80-84 degrees fahrenheit, and see if that fixes your stalled growth.


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Re: WBS Moisture Content? [Re: Burke Dennings]
    #4010466 - 04/03/05 11:34 PM (16 years, 8 months ago)

I just dont really have the space for an incubator. Im kind of doing this stealth mode, and an incubator would look really obvious. I guess i may break down, and give it a try.


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InvisibleBurke Dennings
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Re: WBS Moisture Content? [Re: jtk]
    #4010475 - 04/03/05 11:35 PM (16 years, 8 months ago)

There's got to be a way for you to do it discreetly. 4 quarts would fit into a relatively small box. Maybe you could try a heat bomb?


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Re: WBS Moisture Content? [Re: jtk]
    #4010479 - 04/03/05 11:36 PM (16 years, 8 months ago)

is all i need for a small TIT, 2 equal sized tubs, and a submersable fish tank heater? how cheap can this be done for? And are all heaters adjustable, even the ones from wal mart?


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Re: WBS Moisture Content? [Re: jtk]
    #4010482 - 04/03/05 11:37 PM (16 years, 8 months ago)

explain a heat bomb?


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Re: WBS Moisture Content? [Re: jtk]
    #4010484 - 04/03/05 11:37 PM (16 years, 8 months ago)

$15?


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InvisibleCrasher
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Re: WBS Moisture Content? [Re: jtk]
    #4010489 - 04/03/05 11:38 PM (16 years, 8 months ago)

It's a submersible aquarium heater inside of a glass bottle placed inside your chamber.


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Re: WBS Moisture Content? [Re: Crasher]
    #4010500 - 04/03/05 11:41 PM (16 years, 8 months ago)

so a heat bomb is just a glass jar filled w/ water w/ a submersable heater in it set to 83 or so degrees? Just put that into my chamber and it will warm the whole thing to 83?

How big of a heater do i need for this? Like would a heater for 5-10 gallons work?


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InvisibleBurke Dennings
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Re: WBS Moisture Content? [Re: jtk]
    #4010507 - 04/03/05 11:43 PM (16 years, 8 months ago)

You don't need a big heater at all. Do a search on how to build a heat bomb.

Here's a link for a TiT setup tek. Modify it to fit your needs.


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Invisibleagar
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Re: WBS Moisture Content? [Re: jtk]
    #4010629 - 04/04/05 12:10 AM (16 years, 8 months ago)

In another life, I authored that tek. agar=SixTango=Mycota on various sites

Assuming you used multi/spore inoculate"

Incubation temp is appears to be what your trouble is.

Multi/spore can be slow in starting. Constant temps around 80/82 are in the optimal range. See if you can make that happen.



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Offlinejtk
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Re: WBS Moisture Content? [Re: agar]
    #4010651 - 04/04/05 12:16 AM (16 years, 8 months ago)

Yes, agar i think im going to build a heat bomb, and put it in my incubation chamber. Do you think i may have already fucked them up by letting them dry out too much. Or will the increased temp. get them going again?

I pc'd them and waited about 5 days then inoced them, so it's only been like 2 weeks since pc'ing. I dont think that they should have dried out that much.


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Invisibleagar
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Re: WBS Moisture Content? [Re: jtk]
    #4010669 - 04/04/05 12:21 AM (16 years, 8 months ago)

I inoculate within 50 hours of PC'ing. Sooner is better IMHO.

What size hole is in the lid & did you use tyvek? !/4 inch hole & tyvek will not lose a lot of moisture - fast.

If not to dried out, they should still colonize, if you can raise temp to 80F. If you make an incubator, place an open jar of water in it - also. To help the rH, so they don't dry out - anymore.


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Re: WBS Moisture Content? [Re: agar]
    #4010687 - 04/04/05 12:25 AM (16 years, 8 months ago)

I used 3/8 holes with polyfill.


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Re: WBS Moisture Content? [Re: jtk]
    #4010724 - 04/04/05 12:30 AM (16 years, 8 months ago)

Quote:

jtk said:
I used 3/8 holes with polyfill.




Polyfil allows more air/gas/moisture exchange than tyvek. Sadly, yours may be to dry, now. Only time & better incubation temp will tell.


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Re: WBS Moisture Content? [Re: agar]
    #4010729 - 04/04/05 12:32 AM (16 years, 8 months ago)

im guessing there's no way to get more moisture to it now?

Maybe injecting some sterilized water into the jars?


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Invisibleagar
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Re: WBS Moisture Content? [Re: jtk]
    #4010736 - 04/04/05 12:34 AM (16 years, 8 months ago)

Sadly, injecting water most often will cause bacterial bloom.


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