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OfflineJust_a_Shadow
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Spore solution question
    #4009456 - 04/03/05 08:05 PM (13 years, 1 month ago)

I made some spore solution and inside it looks filmy, like theres not just spores, it seems almost as if the spores tried to germinate inside the water? or is this a contam of some sort?


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Offlinescatmanrav
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Re: Spore solution question [Re: Just_a_Shadow]
    #4009462 - 04/03/05 08:06 PM (13 years, 1 month ago)

Could be either. What sort of water did you use and how did you sterilize.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

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InvisibleHolydiver
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Re: Spore solution question [Re: Just_a_Shadow]
    #4009470 - 04/03/05 08:08 PM (13 years, 1 month ago)

Do you mean 1) You made a syringe from a print? or 2) You made a liquid culture solution from spores?

With a spore syringe it's common to see clumping of the spores, which gives a filmy/cloudy appearance.


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OfflineJust_a_Shadow
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Re: Spore solution question [Re: Holydiver]
    #4009493 - 04/03/05 08:14 PM (13 years, 1 month ago)

oh its just spores in filtered/boiled water (cooled first) from a mushroom cap that hung on a hook. I does look quite cloudy though, I don't think you can see a contam inside this kinda thing eh?


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InvisibleHolydiver
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Re: Spore solution question [Re: Just_a_Shadow]
    #4009498 - 04/03/05 08:16 PM (13 years, 1 month ago)

Need a pic to really give you a positive answer, but it sounds like spores to me.


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OfflineJust_a_Shadow
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Re: Spore solution question [Re: Holydiver]
    #4009509 - 04/03/05 08:18 PM (13 years, 1 month ago)

ok give me a sec, I'll take one.

edit: Ohh ok, never mind, I dont have the cam on hand

but I've seen this kinda thing in an ordered spore syringe, I thought it was no good :/


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OfflineKaptKid
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Re: Spore solution question [Re: Just_a_Shadow]
    #4009528 - 04/03/05 08:24 PM (13 years, 1 month ago)

Only one way to find out.inoculate a cake.



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OfflineJust_a_Shadow
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Re: Spore solution question [Re: KaptKid]
    #4009542 - 04/03/05 08:28 PM (13 years, 1 month ago)

haha, that is so true, Then I shal do that :laugh:


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Invisibleagar
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Re: Spore solution question [Re: Just_a_Shadow]
    #4009845 - 04/03/05 09:40 PM (13 years, 1 month ago)

If you made the syringe awhile back? Some of the spores may have greminated. If so? You will see little tiny wispy threads of myc inside the syringe. Still good to go. In fact, that leads to faster colonization.


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OfflineJust_a_Shadow
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Re: Spore solution question [Re: agar]
    #4020945 - 04/06/05 11:32 AM (13 years, 1 month ago)

thats what I had in mind too, but I was not sure, I should put this in the fridge I would think.


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