Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMobius_Strip
Distant Relative
 User Gallery

Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 17 years, 10 months
Re: Mycelium in test tube/long term suspension [Re: THEDANGLER]
    #4034290 - 04/09/05 12:58 PM (19 years, 1 month ago)

Hey man, I wasn't thinking it was a stupid question, really. The only time I think a question is stupid is when you've asked 100x and not listening to the answer. It just struck me as funny and not in a condescending way.

Anyway, I'm not sure how this works completely but maybe someone else can elucidate further. My understanding is that the vaccuum isn't important for this process. I think that it won't kill the mycelium but puts it in a vegetative state of suspended animation. I think this will happen vacuum or not. Don't take that as truth though. I don't know what I'm talking about really. This is a new preocess for me as well and I'm going by a memory from a thread I can't seem to find.


--------------------
The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky

Edited by Mobius_Strip (04/09/05 01:00 PM)

Extras: Filter Print Post Top
Offlinedebianlinux
Myconerd - DBK
Male User Gallery

Folding@home Statistics
Registered: 12/09/02
Posts: 8,334
Loc: Over There
Last seen: 10 months, 19 days
Re: Mycelium in test tube/long term suspension [Re: Mobius_Strip]
    #4034614 - 04/09/05 02:37 PM (19 years, 1 month ago)

the vacuum will not hurt the culture.

Extras: Filter Print Post Top
OfflineMarkostheGnostic
Elder
Male User Gallery

Registered: 12/09/99
Posts: 14,279
Loc: South Florida Flag
Last seen: 3 years, 3 months
Re: Mycelium in test tube/long term suspension [Re: MonkeyPod]
    #4037304 - 04/10/05 01:47 PM (19 years, 1 month ago)

Agar-slants - test tubes cooled while lying almost flat thereby creating a long elliptical surface, when covered with mycelium, can be stored for years under sterilized mineral oil. To re-harvest, the mineral oil is drained off in a sterile [glove] box, the agar macerated with a sterile glass rod or scalpel, and the mycelium will reestablish itself for further innoculations. I've kept such tubes in the refrigerator for years. They do not always work, but what does?


--------------------
γνῶθι σαὐτόν - Gnothi Seauton - Know Thyself

Extras: Filter Print Post Top
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 6 days
Trusted Cultivator
Re: Mycelium in test tube/long term suspension [Re: MarkostheGnostic]
    #4037342 - 04/10/05 02:00 PM (19 years, 1 month ago)

You'll have better results using bits of myc colonized grains stored under distilled water. Theres been alot of research on this and this has taken the place of mineral covered agar believe it or not. I personally haven't tested it but from what I've read it's been proven to be superior.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
InvisibleTHEDANGLER
The Mushroom Tip
 User Gallery

Registered: 11/01/04
Posts: 312
Re: Mycelium in test tube/long term suspension [Re: hyphae]
    #4037483 - 04/10/05 02:58 PM (19 years, 1 month ago)

what is i used a little liqiud culture mixed with some distilled water put into a vacutainer...


--------------------
TONS OF SPORES TO TRADE PM ME!!!!

Extras: Filter Print Post Top
OfflineMarkostheGnostic
Elder
Male User Gallery

Registered: 12/09/99
Posts: 14,279
Loc: South Florida Flag
Last seen: 3 years, 3 months
Re: Mycelium in test tube/long term suspension [Re: hyphae]
    #4038477 - 04/10/05 07:50 PM (19 years, 1 month ago)

Well, I'm old tec because I'm old :smile: One would think that any grain surface not covered in mycelium would deteriorate in water. It's been a long time since I dabbled in this sort of thing. Starting from spore-print once and a while seems the thing.


--------------------
γνῶθι σαὐτόν - Gnothi Seauton - Know Thyself

Extras: Filter Print Post Top
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 6 days
Trusted Cultivator
Re: Mycelium in test tube/long term suspension [Re: MarkostheGnostic]
    #4038614 - 04/10/05 08:21 PM (19 years, 1 month ago)

Ya theres been alot of changes since my first homestead kit back in the 70's :wink: The myc continues to colonize the grain even under the water just at a much slower rate. GL guys


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Extras: Filter Print Post Top
OfflineMarkostheGnostic
Elder
Male User Gallery

Registered: 12/09/99
Posts: 14,279
Loc: South Florida Flag
Last seen: 3 years, 3 months
Re: Mycelium in test tube/long term suspension [Re: hyphae]
    #4040052 - 04/11/05 05:45 AM (19 years, 1 month ago)

Hey cool! I have a stolen poster for the "No B.S. Homestead Mushroom Growing Kit." Yeah, no 'bovine shit' just a neat acrylic bucket, spores and casing. It didn't work for me and then Prince George's County, MD outlawed head-shops and I couldn't exchange the kit - the store was gone.


--------------------
γνῶθι σαὐτόν - Gnothi Seauton - Know Thyself

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* How should I seal off my test tubes for coffee experiment Gr0wer 1,302 10 05/15/04 02:29 PM
by Gr0wer
* test tube question x_bonerz_x 1,040 9 02/25/03 07:22 AM
by x_bonerz_x
* Storing mycelium in liquid nitrogen ?
( 1 2 all )
ragadinks 6,334 27 05/31/04 01:01 AM
by Speeker
* Experience with long-term storage in distilled water?
( 1 2 all )
ragadinks 7,292 28 04/11/24 08:55 AM
by Shroomin4u
* practical method for long term storage of mycellium in dH2O
( 1 2 all )
Quincunx 9,323 23 02/15/04 01:23 PM
by Anno
* An Experiment In Long(ish) Term Culture Storage aural 1,069 2 08/15/02 10:44 AM
by DinoMyc
* Honey mycelium... wikedanjel 2,942 8 07/30/02 09:05 AM
by CaliChronic
* A dead horse subject: Liquid Mycelium, a new approach?
( 1 2 all )
doctor_kandee 7,190 24 09/23/05 06:46 PM
by doctor_kandee

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
4,661 topic views. 1 members, 2 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 14 queries.