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Offlines3x3s
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Registered: 03/11/05
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Last seen: 11 years, 10 months
No signs of growth on day 4... am I hosed?
    #4005195 - 04/02/05 04:10 PM (11 years, 10 months ago)

I inoculated 9 pf cakes w. p.cube-burma Tuesday night. It is likely that the cakes were over heated for a short period of time. (12-24 non consecutive hours) the air temp at one point was 110 coming out of the fan, however the temp between the jars was only 94. * I am aware that both of these temps are way to high, and have since moved on to TiT *

In short I am working if there is any chance that they are still growing, it has been 4 days with nary a sign of growth, except for what looks like a contam in one of my jars which hasn't really changed size or shape.
Would lack of moisture halt growth w. out seriously hurting the spores?
Thank you.
-s3x3s


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InvisibleRapmaster
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Registered: 09/19/04
Posts: 414
Re: No signs of growth on day 4... am I hosed? [Re: s3x3s]
    #4005199 - 04/02/05 04:12 PM (11 years, 10 months ago)

it took me two weeks to see anything growing in my first cakes. If nothing in a month, toss.


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OfflinekronnyQ
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Re: No signs of growth on day 4... am I hosed? [Re: s3x3s]
    #4005201 - 04/02/05 04:13 PM (11 years, 10 months ago)

Patience young jedi...i've seen myc take up to 8 days to show.

Also lack of moisture will SERIOUSLY hurt growth...if you messed up the water content in your jars myc may never show.


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InvisibleDerGeist
Completing theCycle

Registered: 12/23/04
Posts: 135
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Re: No signs of growth on day 4... am I hosed? [Re: s3x3s]
    #4005204 - 04/02/05 04:14 PM (11 years, 10 months ago)

Give it time.. Too dry will slow things, but 4 days isn't much.. I would say give it another 4, if nothing has happened, then you might think about scrapping it. But a Foaf has seen it take up to 7 days to see anything, and I have read here, tales of much longer.. Could be they will need time to recover from the heating. They don't seem to like temp swings when they are growing, slows them down some. Nice even temps, and to be left alone..

Be Patient,
DG


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Offlines3x3s
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Re: No signs of growth on day 4... am I hosed? [Re: DerGeist]
    #4005221 - 04/02/05 04:25 PM (11 years, 10 months ago)

*a note on my inoculation tek, I figured that it would be more prudent to use 5 holes instead of 4, to facilitate colonization. I have 4 holes spaced evenly around the rim, and one in the center.
I have it around 78 ( tit isn't getting as high as i would like it, around 83, 100watt not a 200 )
I just dissected the cake I figured was contam, Can anyone advise me what a healthy cake will smell like, Because the cake I just opened up smelt horrible. Sort of like used cat litter.

Also the cake was fairly dry, about the consistency of sponge cake, but not dust. I am going to attempt to rehydrate via sterile syringe.
-s3x3s


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Offlineandyspof
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Re: No signs of growth on day 4... am I hosed? [Re: s3x3s]
    #6137197 - 10/05/06 08:07 PM (10 years, 4 months ago)

Ahhhh, i see your using Burma, i bought Burma because it was supposedly supposed ti be one of the fastest colonizing strains, but it was the slowest i have ever had. I had it at constant 84 degrees and it took 3 and a half weeks to colonize. I inoculated jars with PESA a week after i inoculate my Burma jars and the pesa jars finished 4 days earlier.


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Offlinemushnoon
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Re: No signs of growth on day 4... am I hosed? [Re: andyspof]
    #6137218 - 10/05/06 08:12 PM (10 years, 4 months ago)

^^^that may or may not be strain related. you could have had too much moisture. too much moisture can greatly slow things down.


--------------------
I love the feeling when it falls apart. Im slow to finish but im quick to start.


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Offlineandyspof
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Re: No signs of growth on day 4... am I hosed? [Re: mushnoon]
    #6137220 - 10/05/06 08:13 PM (10 years, 4 months ago)

Could be, i really dont think so because i also inoculted some z strain into cakes from the same substrate batch as my Burma cakes and the z strain colonized in normal time.


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InvisibleTheEnd
Strange Daze

Registered: 09/05/06
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Re: No signs of growth on day 4... am I hosed? [Re: andyspof]
    #6137228 - 10/05/06 08:16 PM (10 years, 4 months ago)

Dude....old thread.


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Offlineandyspof
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Registered: 10/10/05
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Re: No signs of growth on day 4... am I hosed? [Re: TheEnd]
    #6137334 - 10/05/06 08:52 PM (10 years, 4 months ago)

Quote:

g2_93integra_boy said:
Dude....old thread.




Lol, shit somehow i came upon this post, and seeing how quickly i came upon it i figured it was recent. My bad.... didn't check the date.


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