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Avid Learner
Registered: 02/02/05
Posts: 2,803
Few Problems and Questions
    #3998276 - 04/01/05 01:38 AM (13 years, 27 days ago)

Ok, couple of questions. AFOAF made up some liquid cultures (malt/dextrose) and noc'd them up with several different strains, most from the same vendor. 50% of the strains are doing amazing. 25% show a little myc growth but nothing like the first 50%. 25% are not showing any myc growth at all. It has been 12 days.

Do you think the 25% not showing growth came from bad syringes or bad jars?

A couple of the strains that aren't showing growth in LC were also put into PF jars as spore innoculant. Maybe 10% show some sign of myc growth but not much at all. The rest are not showing any growth. Should my friend get rid of the jars of this strain and just innoc them with a good healthy strain from a LC? Should they contact the vendor or write it off as a loss?

All of my friends jars that were inoculated with LC are doing pretty well. Some of the jars inoculated with spores appear to be almost stalled. How long should he wait to see if they fully colonize?

A couple of the jars got some water in them when PCing. The bottom of the jars turned to mush. Should my friend leave the jars to see if the myc can fight their way into the compact substrate? Birth the cake as soon as it stalls and get rid of the uncolonized substrate? or just restart the jar?

What is the normal colonization time for a 1/2 pint PF jar using spores? using LC? Is it uncommon to have jars around 95-100% colonized after 12 days of being innoc'd with LC? Is 12 days extremely long to show less than 20% growth from spores?

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Da Bud Guru
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Registered: 07/25/02
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Re: Few Problems and Questions [Re: Smushroom]
    #3999639 - 04/01/05 12:20 PM (13 years, 27 days ago)

Contact the vendor and see what they have to say after you tell em what happen.

All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?

:growingweed: Join us at the Growery! :growingweed:

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Registered: 02/18/05
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Re: Few Problems and Questions [Re: Magash]
    #3999684 - 04/01/05 12:30 PM (13 years, 27 days ago)

whats does AFOAF mean? i keep reading people saying that and talking in third person??

There must be some kinda way outta here said a joker to the thief.

Edited by ralphie (04/01/05 12:30 PM)

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Chicks can do it too!
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Re: Few Problems and Questions [Re: ralphie]
    #3999692 - 04/01/05 12:31 PM (13 years, 27 days ago)

A Friend Of A Friend.
Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:

Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse

My First Pans

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