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Offlines3x3s
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Registered: 03/11/05
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Just did the pf tek for the first time, having a few problems.
    #3998228 - 04/01/05 01:27 AM (11 years, 8 months ago)

problem 1, which is not a problem really, I used the simple pf tek to the T and had enough substrate for 9 jars. that was odd, I did pack them extra loose thought and this might account for it.

My main problem/question. Scenario
Under my bed I have my freshly inoculated cakes ( Tuesday night ) the first night I had a cheap heater under my bed set to 775wats My guess is that it had reached temps of 105. i unplugged it in the afternoon ( Wednesday ) and procured a heater w/ a temp control, i set it for 85. which apparently means 110 when the fan is on. I acquired a digi thermometer and the temp between the jars is ~85. the temp w/ the heater on (it says 85, however my thermometer reads 89 between the jars )
First part, Did I fry my spores?
B, is it likely that i zapped too much moisture in the jars?
C, if too much moisture has been lost, is it possible to inject sterile water in via the inoculation holes w. a low risk of contamination?
Thank you
s3x3s


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Offlinejsims88
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Re: Just did the pf tek for the first time, having a few problems. [Re: s3x3s]
    #3998248 - 04/01/05 01:31 AM (11 years, 8 months ago)

well if it reached that high of a temp its possible that you zapped the spores also. it is possible to inject water into the jars use bottles water and sertle syg. also 89 is a little high , 82-85 ideal.

plus its a few degrees warmer inside the Jars.. GL


Edited by jsims88 (04/01/05 01:32 AM)


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Offlines3x3s
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Re: Just did the pf tek for the first time, having a few problems. [Re: jsims88]
    #3998272 - 04/01/05 01:37 AM (11 years, 8 months ago)

Ok, I have it stabilized between 86.2 when the heater is on. and 80 when the heater is off.

any guesses how long it will be before I can tell if the spores are zapped?
s3x3s


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OfflinebioTek
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Registered: 10/01/03
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Re: Just did the pf tek for the first time, having a few problems. [Re: s3x3s]
    #3998375 - 04/01/05 02:20 AM (11 years, 8 months ago)

if u mean an air heater, then your cakes are, or will be, dried out. if u do get growth, it will pobably stop b4 the cake is fully colonized. i would exchange the new air heater for a submersable aquarium water heater for around $18 or so.


Edited by bioTek (04/01/05 02:26 AM)


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Offlines3x3s
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Re: Just did the pf tek for the first time, having a few problems. [Re: bioTek]
    #3998430 - 04/01/05 02:40 AM (11 years, 8 months ago)

Would you suggest having it submerged ( im sure this is a stupid question ) and if so, in how much water?
would it radiate enough heat if the tank was at 80 degrees? or would I have to have it in a show basin?
thank you
s3x3s


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OfflinebioTek
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Re: Just did the pf tek for the first time, having a few problems. [Re: s3x3s]
    #3998437 - 04/01/05 02:45 AM (11 years, 8 months ago)

search for the tub-in-tub incubator method.


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Offlines3x3s
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Re: Just did the pf tek for the first time, having a few problems. [Re: bioTek]
    #3998480 - 04/01/05 03:20 AM (11 years, 8 months ago)

would a heat bomb suffice, or should i go ahead and go w. the tit? I have 9 pf-teks under my bed. a space of 2ft in height and 1.5 ft in width, depth is 6ft... but doesn't really matter.
s3x3s


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Offlinejsims88
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Re: Just did the pf tek for the first time, having a few problems. [Re: s3x3s]
    #3999100 - 04/01/05 10:04 AM (11 years, 8 months ago)

if this is for the incubater , use a heating Pad or Seed mat. Maintains constant temps and its not a Freaking Heater.. JUSt try that or like they says , Tub in tub always works , Also . you will know spores are toast when nothing grows.


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Offlinedr0mni
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Re: Just did the pf tek for the first time, having a few problems. [Re: jsims88]
    #3999448 - 04/01/05 11:33 AM (11 years, 8 months ago)

Yeah, use an under-the-tank heating pad stuck to the bottom of a rubbermaid container. Simple. This is what I do and it's been working great! Temps are almost perfect ~80F and it fits nicely into my nightstand drawer.


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Offlines3x3s
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Re: Just did the pf tek for the first time, having a few problems. [Re: dr0mni]
    #4001586 - 04/01/05 06:48 PM (11 years, 8 months ago)

can anyone provide a TiT-tek link, I cant find it anywhere.
Thanks.
s3x3s
PS
It looks like C1 is contaminated, ( day 2 ) and C4, C6, C8 may possibly be showing signs of myc growth.


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InvisibleRoadkill
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Re: Just did the pf tek for the first time, having a few problems. [Re: s3x3s]
    #4001606 - 04/01/05 06:53 PM (11 years, 8 months ago)

here is one link

Click here!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineShrooms4Less
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Re: Just did the pf tek for the first time, having a few problems. [Re: Roadkill]
    #4007443 - 04/03/05 03:35 AM (11 years, 8 months ago)





--------------------
If your friend is already dead, and being eaten by vultures, I think it's okay to feed some bits of your friend to one of the vultures, to teach him to do some tricks. But ONLY if you're serious about adopting the vulture. - Deep Thoughts (Saturday Night Live)


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InvisibleRoadkill
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Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: Just did the pf tek for the first time, having a few problems. [Re: Shrooms4Less]
    #4008748 - 04/03/05 04:27 PM (11 years, 8 months ago)

I need more cow bell baby!~

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Amazon Shop for: ½ Pint Jars

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