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Offlinepsilocyben
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last second spawn question
    #3997860 - 03/31/05 11:54 PM (12 years, 6 months ago)

the tenn stud order arrived, and i just wanted to be sure that everything was clear.

3 qts. wbs
10+ qts of poo/verm

should it be thouroughly mixed together, or layered;
exp. has been layering, but straw was included, so this was necessary.

first time w/ plain old quality H; just want to make sure everythung is optimum.

thanks guys-
psilocyBen


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OfflineHippieChick
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Re: last second spawn question [Re: psilocyben]
    #3997881 - 04/01/05 12:00 AM (12 years, 6 months ago)

With the Tennesse Stud substrate, its recommended at a 10:1 substrate to spawn. Mix it well together and then apply a layer of your WBS on top of your substrate. This layer will colonize quickly to help ward off contam's. Place in incubator for 7-10 days. When fully colonized, case and innitiate pinning. Good Luck.
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Offlinepsilocyben
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Re: last second spawn question [Re: HippieChick]
    #3997915 - 04/01/05 12:08 AM (12 years, 6 months ago)

i saw the info he had on his site, but i honestly am looking for a little bit different spawn ratio than 1:10.
though the birdseed is 110% col., (rhizo's) when it breaks up, it still seems that there is exposed grain.
just wondering if there should be a layer of straight poo on top?... or if i'm paranoid/ confused about proper methods.

thanks


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OfflineHippieChick
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Re: last second spawn question [Re: psilocyben]
    #3997927 - 04/01/05 12:13 AM (12 years, 6 months ago)

You want a layer of colonized mycelium on top. It's contam resistant. A layer of straight poo would probably work as a breeding ground for contam's. All the poo TEK's I have read say to do this.
Good Luck.
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:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


Edited by HippieChick (04/01/05 03:09 PM)


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InvisibleHolydiver
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Re: last second spawn question [Re: HippieChick]
    #3997998 - 04/01/05 12:34 AM (12 years, 6 months ago)

What you have is fine, that's 30% spawn to poo. 4 quarts to 10 LBS. is about the ratio I run. Yeah it's overkill, but better to have your substrate colonize uber fast if you can help it. Make sure your substrate is nice and level when finished mixing, this will come in handy when you go to apply an even casing layer.



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OfflineHippieChick
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Re: last second spawn question [Re: Holydiver]
    #3998064 - 04/01/05 12:47 AM (12 years, 6 months ago)

That's cool, Diver. I was wondering about that when I checked out the Tenn. Stud substrate. Wanted to use some more WBS myself. How much time do you think it could take off colonizing? Couple day's?
But, isn't 5 pounds of colonized poo 5 pounds of colonized poo regardless of how much spawn you used? Or would starting with more WBS give you a thicker mycelium growth? Just wondering?
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:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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InvisibleHolydiver
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Re: last second spawn question [Re: HippieChick]
    #3998102 - 04/01/05 12:53 AM (12 years, 6 months ago)

More spawn definitely cuts off on the colonization time. Definitely days. But just as important is getting as many inoculation points as possible (mixing the spawn in correctly).

Good question, I've been wondering this myself. I'd have to say 5 LBS of poo is 5 LBS of poo, regardless of how much spawn was used to get it there.

Some people believe that the more WBS/RYE used to spawn the bulk substrate, that the more nutrition you are establishing as base. I somewhat see some truth to this, but feel that you would literally need 50/50 grain/poo to really see a difference.


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Offlinepsilocyben
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Re: last second spawn question [Re: Holydiver]
    #3998493 - 04/01/05 03:33 AM (12 years, 6 months ago)

just to clarify, then diver-
it looks like there is no exposed spawn in your sedcond pic.
could you clarify whether or not there is a top layer of poo, or if you are to chop up the wbs into smaller sections and thouroughly mix.

just did previously explained ratio w/ it mixed togetherw/ spawn layer on top, as suugested above.

pasteurizing some more, and looking to ensure my methods are correct.

thanks


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InvisibleHolydiver
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Re: last second spawn question [Re: psilocyben]
    #3999023 - 04/01/05 09:25 AM (12 years, 6 months ago)

Quote:

psilocyben said:
just to clarify, then diver-
it looks like there is no exposed spawn in your sedcond pic.
could you clarify whether or not there is a top layer of poo





Hey,

There is exposed spawn in the 2nd pic, look closer.  It's evenly mixed in, no special attention to layers or anything.  Just mix, cover and incubate.  :smile:


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Invisibleagar
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Re: last second spawn question [Re: HippieChick]
    #3999103 - 04/01/05 10:06 AM (12 years, 6 months ago)

Look at VOLUME.... not weight in spawn/substrate ratio's.

Higher spawn ratio's ARE BETTER, up to a point (30% IMHO).

The object of spawn (besides nutriants) is to introduce myc into an uncolonized substrate, which it then - colonizes.

The more spawn, the more points colonization begins from, all moving towards one another. The more spawn introduced, the closer the contact points - become. Which results in FASTER COLONIZATION, as the myc doesnt have to travel far - to connect with it's counterparts.

As H/C noted, top dressing a few percent more spawn, allows the top to colonize faster, creating a protective layer over uncolonized substate (a good thing).

As myc colonizes spawn grains, it knits the grain togather, the myc being the conective tissue.

The end result is solid mass of grains in a jar, you have to dig into & break apart... in order to seperate the individual grains. Doing so - injures all the myc conective tissue.

So, the spawn is not at 100% efficiancy, until it heals itself (takes a day or 2), then begins to grow, move into & colonize its new surroundings.

If you use spawn BAGS? Two (2) days (36 to 48 hours) before you intend to spawn it into a bulk substrate. Gently massage the bags, to break the mass into individual grain. They will begin to heal, but are not able to knit the bag of grain back into a solid mass in a 48 hours.

Then - spawm - as quickly & gently as possible (otherwise you re-injure it & defeat the purpose). This method usualy decreases colonization times by a day or 3.


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Offlinepsilocyben
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Re: last second spawn question [Re: agar]
    #3999253 - 04/01/05 10:43 AM (12 years, 6 months ago)

thanks guys...
*bell sounds

whelp, here goes round two.


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Offlinescatmanrav
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Re: last second spawn question [Re: psilocyben]
    #3999873 - 04/01/05 01:18 PM (12 years, 6 months ago)

'Some people believe that the more WBS/RYE used to spawn the bulk substrate, that the more nutrition you are establishing as base. I somewhat see some truth to this, but feel that you would literally need 50/50 grain/poo to really see a difference. "

You normally use a high spawn ratio, but try it with about 1-5% spawn ratio and see if you still feel that way. I tried to cut a few corners because I was low on spawn my last run..all colonizes fine, but yeilds were so poor..no less then 25% again for me...


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleHolydiver
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Re: last second spawn question [Re: scatmanrav]
    #4000026 - 04/01/05 01:44 PM (12 years, 6 months ago)

Quote:

scatmanrav said:
You normally use a high spawn ratio, but try it with about 1-5% spawn ratio and see if you still feel that way.




Interesting, I'll have to try that sometime.


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Invisiblehammer_head
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Re: last second spawn question [Re: Holydiver]
    #4000295 - 04/01/05 02:36 PM (12 years, 6 months ago)

drip drip


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InvisibleHolydiver
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Re: last second spawn question [Re: hammer_head]
    #4000337 - 04/01/05 02:43 PM (12 years, 6 months ago)

Quote:

hammer_head said:
drip drip




What?


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OfflineSoK
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Re: last second spawn question [Re: Holydiver]
    #4000378 - 04/01/05 02:51 PM (12 years, 6 months ago)

Quote:

Diver said:
Quote:

hammer_head said:
drip drip




What?




lolzzz bout to ask the same thing


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