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OfflineNextKind
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Contams 3 days from innoc.?
    #3995872 - 03/31/05 05:10 PM (13 years, 1 month ago)

A foaf cooked 24 half pint BRF jars @ 10 psi for about 10-20 mins each. (Little background info). He checked his incubator today (3 days from innoc) and there was alot of noticable growth, he even said one jar was about 40%. Anyways, he noticed that on 6 of the jars there was a little green dot the size of the tip of an ink pen, amongst the mycelium. Are these contams already?! Should he seperate these jars from the rest in the incubator? Right now he has a towel covering the contaminated jars in the incubator, and the non-contamed ones uncovered, so as to seperate them somewhat. Help!  :sad:


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OfflineRogerRabbitM
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Re: Contams 3 days from innoc.? [Re: NextKind]
    #3995895 - 03/31/05 05:20 PM (13 years, 1 month ago)

You must throw out those that are already green. Three days from inoculation is a bit too early to see much mushroom mycelial growth. This is about when the spores should start germinating. If any jars are 'very far' along, it's almost a sure bet it's contaminants you're seeing and not mushroom mycelium. It sounds like you didn't get the brf sterilized. Ten to twenty minutes seems a bit light, depending on how full they were packed into the pc.
RR


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OfflineNextKind
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Re: Contams 3 days from innoc.? [Re: RogerRabbit]
    #3996386 - 03/31/05 06:48 PM (13 years, 1 month ago)

I already got him to throw them out before you posted, but im positive that everything else is healthy mycelium.. he said the temps are constant at 80-85 so maybe that's why they're growing so well
There were 5 sterilized at a time, somtimes 4


Edited by NextKind (03/31/05 06:49 PM)


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OfflineBloodNOil
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Re: Contams 3 days from innoc.? [Re: NextKind]
    #3998168 - 04/01/05 01:12 AM (13 years, 1 month ago)

10 - 20 minutes? that's not nearly enough.

You need at least three times that.


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