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 rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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 Newbie difficulties 
#3991730 - 03/30/05 08:08 PM (13 years, 28 days ago) |
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I have tried twice with limited success. I have been using the PF-TeK (from this site) and very carefully following the instruction for the mix of ingredients, temps, etc. The first attempt suffered from mold, and I increased my attentiveness to cleanliness. My second attempt had no mold, but the spores simply did not colonize. I uses spore syringes, and 5 jars with 4 squirts in each, and had only two of of the 20 injections produce anything. I want to try again, but am not sure what to change.... some questions.
Do the spore syringes go bad? I purchased these several weeks earlier (for the first attempt) and did not refigerate. How long should they last? Should I refigerate or use right away next time?
I purchased the spores from SporeWorks. Is it possible to just get a bad batch? has this happened to anyone?
Any other suggestions? as I said I was quite careful about moisture, and temperatures so I do not think that was the problem.
any suggestions will be appreciated.
Thanks, Rick
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 DerGeist
Completing theCycle
Registered: 12/23/04
Posts: 135
Loc: The Other Side
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Re: Newbie difficulties [Re: rickdog]
#3991760 - 03/30/05 08:12 PM (13 years, 28 days ago) |
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Give everyone in great detail what you did, and what you are seeing. Setup type, procedures, Incubating Setup, Temps, etc.. etc.. Lots of Devils in the Details here..
DG
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sludge
blunt rapter
Registered: 02/23/05
Posts: 707
Loc: somewhere in usa
Last seen: 12 years, 9 months
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Re: Newbie difficulties [Re: DerGeist]
#3991783 - 03/30/05 08:16 PM (13 years, 28 days ago) |
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ya, its deffently something your doing wrong please explain.
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Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man 
Who won't let himself be
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dog
straw dog
Registered: 06/20/02
Posts: 2,790
Loc: Route 66
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Re: Newbie difficulties [Re: rickdog]
#3991786 - 03/30/05 08:16 PM (13 years, 28 days ago) |
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We need the details of your technique. How you prepped and sterilized your jars, how you inoculated, incubated, etc.
Vendors have sent faulty syringes, but 99% of the time the problem lies with the inexperienced cultivator.
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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.
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ShroomGuy86
Shroooooooooooooooom
Registered: 02/06/05
Posts: 1,048
Loc: MycVille
Last seen: 12 years, 11 months
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- [Re: dog]
#3991800 - 03/30/05 08:20 PM (13 years, 28 days ago) |
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-
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"I put Lamborghini doors on that Es-co-lade
Low pro so low look like I'm riding on blades" - 50 Cent
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rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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Yes, I was not relating the exact details since I had religously followed the directions from this site. I used 5 1 pint jars with brown rice and vermiculite with equal flour and water and double the vermiculite. I sterlized in a pressure cooker for 60 minutes in a pressure cooker at 15psi and let cool over night. I injected 1 cc through four separate holes towards the edge of the jar and below the half inch of pure vermiculite I left on top.
I placed the jars in a plastic tub set in a second plastic tub with water. I left an aquarium heater in the water set at 85 degrees. I also used a digital thermometer to monitor the temps which remained between 80 and 85 degrees. I left the jars for more than three weeks (no mold) before concluding that not much was happening.
What did I do wrong?
Thanks, Rick
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Newbie difficulties [Re: rickdog]
#3991865 - 03/30/05 08:36 PM (13 years, 28 days ago) |
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did you shake the syringes for a few minutes before inoculation?
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sludge
blunt rapter
Registered: 02/23/05
Posts: 707
Loc: somewhere in usa
Last seen: 12 years, 9 months
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Re: Newbie difficulties [Re: Prisoner#1]
#3991892 - 03/30/05 08:41 PM (13 years, 28 days ago) |
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and did you use foil for the jar and not take it off till inculation?
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Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be
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rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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Re: Newbie difficulties [Re: Prisoner#1]
#3991893 - 03/30/05 08:41 PM (13 years, 28 days ago) |
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Hmmmm... well, I think so. certainly will next time. If my technique looks sound I will just try again. Does anyone know the shelf-life of unrefigerated spore syringes? what ever the case I am ordering free syringes for try number three.
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ShroomGuy86
Shroooooooooooooooom
Registered: 02/06/05
Posts: 1,048
Loc: MycVille
Last seen: 12 years, 11 months
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"I put Lamborghini doors on that Es-co-lade
Low pro so low look like I'm riding on blades" - 50 Cent
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rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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Re: Newbie difficulties [Re: rickdog]
#3991922 - 03/30/05 08:46 PM (13 years, 28 days ago) |
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Hard to remember to relay all the details. I used lids with drilled holes and two layers of foil. I pressure cooked the jars with the two layers. Removed the first layer and injected the spores and then replaced the second layer, but did not secure in an air-tight manner. So I have one later with syringe holes that is secured with rubber bands, and then the second layer has no holes and just sets on top.
Thanks!!
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dog
straw dog
Registered: 06/20/02
Posts: 2,790
Loc: Route 66
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Re: Newbie difficulties [Re: rickdog]
#3991925 - 03/30/05 08:46 PM (13 years, 28 days ago) |
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The biggest problem noobs seem to have is with water content. This is because different grades of verm will hold different amounts of water. Try following the PF tek listed here. It compensates for different grades of verm.
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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.
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rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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1 cc per hole
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sludge
blunt rapter
Registered: 02/23/05
Posts: 707
Loc: somewhere in usa
Last seen: 12 years, 9 months
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Re: Newbie difficulties [Re: rickdog]
#3991960 - 03/30/05 08:55 PM (13 years, 28 days ago) |
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Quote:
rickdog said:
1 cc per hole
what!!!!  thats where you fucked up 1cc per jar dude to much thats 4 cc per jar water content fucked up!!!!! 
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Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be
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rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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Re: Newbie difficulties [Re: dog]
#3991966 - 03/30/05 08:56 PM (13 years, 28 days ago) |
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Thanks for the link... not much different from what I used before, but it is very clear, and explicit. I will follow it attentively and hope for the best.
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sludge
blunt rapter
Registered: 02/23/05
Posts: 707
Loc: somewhere in usa
Last seen: 12 years, 9 months
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Re: Newbie difficulties [Re: sludge]
#3991967 - 03/30/05 08:56 PM (13 years, 28 days ago) |
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and the foil thing leave it on till you inculate your jars and then take it off then put in the incubater!!!
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Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be
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rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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Re: Newbie difficulties [Re: sludge]
#3991998 - 03/30/05 09:01 PM (13 years, 28 days ago) |
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uh..... I guess I was not remembering right. I used a 10 cc syringe for 5 jars and had about 30 to 40% left over, which implies that I used slightly more than 1cc per jar. I did not think water content was that crucial so I will pay more attention to this.
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sludge
blunt rapter
Registered: 02/23/05
Posts: 707
Loc: somewhere in usa
Last seen: 12 years, 9 months
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Re: Newbie difficulties [Re: rickdog]
#3992005 - 03/30/05 09:03 PM (13 years, 28 days ago) |
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was the mold you had wet rot,mistakein for a mold what coler was it
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Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be
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KaptKid
Spaced Pirate
Registered: 12/11/03
Posts: 6,248
Loc: Bright Side of the Sun
Last seen: 1 year, 1 month
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Re: Newbie difficulties [Re: rickdog]
#3992080 - 03/30/05 09:18 PM (13 years, 28 days ago) |
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My first two grows did nothing, cut back on h2o and bam.
Good Luck
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Child of the 60's, Tripping ever since.
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rickdog
Stranger
Registered: 08/28/04
Posts: 10
Last seen: 13 years, 19 days
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Re: Newbie difficulties [Re: sludge]
#3992119 - 03/30/05 09:26 PM (13 years, 28 days ago) |
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The "mold" was a mixture of fuzzy white and green. It did not look like mycelium. I was not careful about cleaning the substrate away from the edge at the top of the jar, and did not leave the half inch of dry verm at the top, so I am guessing that the mold had an entry way into the jar... I was able to cure this with the second attempt, and did manage to fruit about 5 caps total from the one squirt that colonized. Not what I was hoping for.... sounds like water content is the key.
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