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Amazon Shop: Agar, Petri Dish, pH Test Strips, Potato Dextrose

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OfflineAlegalize
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Registered: 02/23/05
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Agar PH.
    #3991224 - 03/30/05 06:02 PM (13 years, 2 months ago)

I?ve inocullated 8 petri dishes of potato dextrose agar and none had any mycelial growth nor contamination. The source are sporeworks syringes that have been on a dark cardbox in a drawer for 8 months. Amazonian, B+ and Jalisco strains. I was wondering if that happened because of the slightly acidic ph (something like 5,6) of the distilled water I used to make the agar. I used peroxide too in the mixture. All following the directions (peroxide concentration and quantities) on the faqs. Put them to incubate in a 24? 30? C dark place. Nothing happens in a month... I?m sad but gotta do this right, so I?ll try it again! Any thoughts?


Edited by Alegalize (03/30/05 06:10 PM)


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Invisiblederx
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Re: Agar PH. [Re: Alegalize]
    #3991236 - 03/30/05 06:05 PM (13 years, 2 months ago)

if you used spores like you said, you shouldn't have used H2O2. H2O2 kills fungi spores as well as other spores. You live and learn, then you get luvs.


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Edited by derx (03/30/05 07:33 PM)


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OfflineRogerRabbitM
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Re: Agar PH. [Re: derx]
    #3991247 - 03/30/05 06:07 PM (13 years, 2 months ago)

I've never seen distilled water as anything but ph7. Why would yours be lower?
RR


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InvisibleOlgualion
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Re: Agar PH. [Re: Alegalize]
    #3991471 - 03/30/05 06:52 PM (13 years, 2 months ago)

You killed your spores with the peroxide. Peroxide cannot be used with spores, only with already growing mycelium...


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OfflineAlegalize
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Thanks... [Re: Olgualion]
    #3992675 - 03/30/05 11:42 PM (13 years, 2 months ago)

So next time working with spores I?ll check for some distilled water with an adequate PH and not use the peroxide and see how It?ll go.
Thanks for the hints....


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Amazon Shop: Agar, Petri Dish, pH Test Strips, Potato Dextrose

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