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Offlinelordoftheshroomz
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using water from a dunk to start a liquid culture?
    #3989847 - 03/30/05 01:19 PM (12 years, 8 months ago)

my question is, do u think that after a dunk there would eb enough mycelial cells dispersed in the water to do something like a water and mycelium syringe or a karo water liquid culture? jus a thought of killing two birds with one stone


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Anonymous

Re: using water from a dunk to start a liquid culture? [Re: lordoftheshroomz]
    #3989865 - 03/30/05 01:27 PM (12 years, 8 months ago)

Yep, a friend came up with this.

http://www.shroomery.org/index/par/24254


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OfflineDrink_Punk_Soda
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Re: using water from a dunk to start a liquid culture? [Re: four20snakeman]
    #3989998 - 03/30/05 01:57 PM (12 years, 8 months ago)

I've always wondered what would happen if, in a clean glovebox, a cake was dunked in karo water instead of plain water.. imagine the flushes if a cake was totally soaked in nutrients!


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InvisibleOlgualion
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Re: using water from a dunk to start a liquid culture? [Re: four20snakeman]
    #3990008 - 03/30/05 01:58 PM (12 years, 8 months ago)

I remember posting **edit* a similar ** idea back in the days of a.d.m. before the shroomery was around. It never got any response...

You don't even need to use karo, just plain old sterile h2o...


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Edited by Olgualion (03/30/05 03:29 PM)


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Offlinegoofy98
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Re: using water from a dunk to start a liquid culture? [Re: Olgualion]
    #3990045 - 03/30/05 02:07 PM (12 years, 8 months ago)

Covering your cake in nutes is a bad idea. VERY BAD IDEA this just gives contams a chance to get a foothold on your cake. You will just cover your cake in a awesome enviroment for trich and everything else in the air. please dont do it


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Offlinegoofy98
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Re: using water from a dunk to start a liquid culture? [Re: goofy98]
    #3990050 - 03/30/05 02:08 PM (12 years, 8 months ago)

dunking in sterile H2o is fine though and is recomended


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InvisibleOlgualion
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Re: using water from a dunk to start a liquid culture? [Re: goofy98]
    #3990084 - 03/30/05 02:14 PM (12 years, 8 months ago)

you are entirely correct, I was just referring to the "Tek" four20snakeman pointed to...


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Anonymous

Re: using water from a dunk to start a liquid culture? [Re: Olgualion]
    #3990399 - 03/30/05 03:19 PM (12 years, 8 months ago)

yeah, that tek was made for using a cake that you dont plan to open. i was just pointing it out to show that the procedure in question was possible, adaptations can be made to suit your needs.


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OfflineDrink_Punk_Soda
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Re: using water from a dunk to start a liquid culture? [Re: goofy98]
    #3990443 - 03/30/05 03:30 PM (12 years, 8 months ago)

I think you missed my point.. dunking a fully colonized cake in sterile caro water in a gloebox and fruiting in a sterile environment would not allow for contamination.

Creating a sterile fruiting environment, on the other hand, would be incredibly difficult admittedly.. but I imagine the results would be impressive.


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