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Amazon Shop for: ½ Pint Jars, Agar, Autoclave

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Invisibleagar
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QUASI-INVITRO CAKES FOR SANITARY PRINTS:
    #3988862 - 03/30/05 04:10 AM (12 years, 1 month ago)

NOTE - This method assumes you can, have or will someday produce PF style cakes & want to make prints.

Are you worried the prints you plan to make someday might end up dirty?

Not a new or perfect method. But, it minimizes cap exposure to airborne contamination.

If you have a gallon size glass jar w/lid around. (if need be - buy gallon glass jar of pickles & eat pickles :grin:)

Have a cake ready to birth.

Drill four ? inch holes through gallon jar lid (near edge) & sanitize both jar & lid..

Insert polyfil wad into lid holes - for air/gas exchange filter.

Place a  spacer (meaning something hollow & round that will elevate cake off lid ? inch - like cut PVC pipe, cut soda can (scissors will cut soda can) - or whatever) on inverted sanitized lid.

Mist distilled water (with a little H202 in it) into gallon jar - until inside glistens - but doesn?t drip a lot..

Birth cake on spacer, on lid.

Lower gallon jar onto lid - spacer - cake & screw on firmly.

Gently move & place jar on mesh or wire grate (anything that will elevate ? inch off flat surface - (to allow for gas/air exchange - via polyfil in lid), situate jar where temp is 74/75F area & gets NO direct sunlight (direct sunlight will over heat jar).

If internal RH gets or appears very low.

Raise jar, remove one polyfil wad, spray a burst or two of distilled water up into jar & replace polyfil wad.

Once in awhile, loosen one polyfil wad, to allow more gas exchange. 02 is heavy & will flow out.

Allow cake to fruit.

Once fruiting & veils break?. Prepare printing containers & whatever you lay print on (foil - whatever).

Move sanitized  printing container, foil  & jar into sanitized glove box (or - sanitized bathroom counter)..

Unscrew lid, raise & remove jar off cake.

Sever caps, lay on foil, cover with print container.

You know the rest (I hope).

agar is lit & off to bed.


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Edited by agar (03/30/05 04:31 AM)


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InvisibleMrMaddHatter
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Re: QUASI-INVITRO CAKES FOR SANITARY PRINTS: [Re: agar]
    #3988925 - 03/30/05 04:51 AM (12 years, 1 month ago)

I've been thinking alot about this same thing for about a year. My idea was going to be to make a quart with only 1"-1 1/2" grain and fruit it in the jar.

I like your idea.

Spores are the "seeds" of the mushrooms. Have to have PLENTY of fresh clean spore prints around.
Alot of us don't own a flowhood, so this sounds like the best way to do it.

I need to get some gallon jars (damn those things are impossible to find in quanity localy :mad2:)

Time to buy some pickles :wink:


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I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.
Hunter S. Thompson

It's always tea-time, and we've no time to wash the things between whiles.

Don't be a fool, this is a war on drugs. Act like. The government sure does!


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Offlinemushroommark
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Re: QUASI-INVITRO CAKES FOR SANITARY PRINTS: [Re: MrMaddHatter]
    #3989009 - 03/30/05 06:01 AM (12 years, 1 month ago)

If anyone has a good source of gallon jars that are autoclaveable please share.  Like to avoid the pickle jars, if at all possible.

Local or online....either would work fine.  I've been keeping an eye out for them for some time and have never seen them thanks

mushymark

Sorry, didn't mean to hijack...it just spiked my interest for a moment there.  Feel free to shoot me a PM. Like always, agar, CLEVER.  "toilet flush simple" :lol:  :wink:


Edited by mushroommark (03/30/05 06:07 AM)


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Invisibleagar
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Re: QUASI-INVITRO CAKES FOR SANITARY PRINTS: [Re: mushroommark]
    #3989014 - 03/30/05 06:17 AM (12 years, 1 month ago)

ON-LINE.......HERE

After you add shipping costs, price is about the same as gallon jars FULL of big dill pickles, from bulk grocery (think CostCo - Smart & Final et al). Gallon glass pickle jars & lids will autoclave.

Then you get all those FREE PICKLES. :rolleyes:


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Offlinescatmanrav
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Re: QUASI-INVITRO CAKES FOR SANITARY PRINTS: [Re: agar]
    #3989497 - 03/30/05 11:24 AM (12 years, 1 month ago)

Ball makes half gallon jugs...someone posted a link before where you can buy quarts and all sized jars...with shipping of course prices arent great but you get actual caning jars.

Of course myself, big fan of pickles...and CostCo.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Amazon Shop for: ½ Pint Jars, Agar, Autoclave

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