Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

World Seed Supply
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineFYP
Stranger
Registered: 09/20/01
Posts: 1
Last seen: 23 years, 6 months
late sterilisation/inoculation
    #398864 - 09/20/01 03:22 AM (23 years, 6 months ago)

I prepared 10 jars to be inoculated, but, due to a problem, I only sterilized them and inoculated them the next day, now it's been 5 days, and still no sign of growing, is this the reason why ? did the substrate get too dry or something ?


Extras: Filter Print Post Top
OfflineKosmokos
Stranger
Registered: 09/01/01
Posts: 6
Loc: U.S.A.
Last seen: 23 years, 3 months
Re: late sterilisation/inoculation [Re: FYP]
    #398873 - 09/20/01 03:39 AM (23 years, 6 months ago)

Just last week we made up a bunch of jars and "because of some problems" we waited a couple days---not thinking about mold---to sterilize and innoculate. Well, needless to say they got all moldy and we had to start all over. Rule of thumb on that one is: Sterilize the same day you make the jars. Otherwise you'll get mold forming.
Now, in your case the mold probably started to form in those 24 hours between creation and sterilization (however it should have been killled off by sterlizing, but I don't know much about mold once it forms) and that MAY have something to do with the fact that they're not colonizing; but I'm not an expert on that one. Temperature is another factor that will determine what rate tthe mycelium grows at. Give 'em another week at least 70 Fahrenheit and if you have nothing by then, I say start over.
Are you sure your spore syringes are good??

"He who's knows others is wise, he who knows himself is enlightened." ---Lao Tzu


--------------------
"He who's knows others is wise, he who knows himself is enlightened." ---Lao Tzu

Extras: Filter Print Post Top
Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: late sterilisation/inoculation [Re: FYP]
    #398892 - 09/20/01 04:41 AM (23 years, 6 months ago)

You ought to wait a few days,then worry.


Extras: Filter Print Post Top
Anonymous

Re: late sterilisation/inoculation [Re: FYP]
    #398906 - 09/20/01 05:30 AM (23 years, 6 months ago)

And try to get those temps up to about 82.

Take a trip to the Spore Lab @:
http://www.SporeLab.com
email: getspores@sporelab.com

Extras: Filter Print Post Top
Invisibleoscill8
*bondage fairy*
Registered: 02/26/01
Posts: 1,215
Re: late sterilisation/inoculation [Re: Kosmokos]
    #399036 - 09/20/01 11:13 AM (23 years, 6 months ago)

kos- many people do wait several days before innoculation, its called the control jar technique. it lets you verify your sterilization of the substrate was successful. if you have nasites growing in there before you innoc., you know you did not sterilize the jars correctly. when prepared properly, nothing will grow in them within those few days. if youre gettimg mold or anything in there before innoc., check your sterilization b/c its not working.


***Please Donate Blood. American Red Cross***


--------------------
? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"

Extras: Filter Print Post Top
Offlinedarkfly
LysergicExperimenter

Registered: 09/16/01
Posts: 1,169
Last seen: 9 years, 7 months
Re: late sterilisation/inoculation [Re: oscill8]
    #399092 - 09/20/01 12:01 PM (23 years, 6 months ago)

I think he let his jars sit for a day before sterilizing them.

darkfly

-- too much trippin and my soul is worn thin


--------------------
Everything Is Blue In This World. The Deepest Shade Of Mushroom Blue.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Tape Over Inoculation Holes? plaastikbob 4,188 10 09/07/01 10:33 AM
by DeathCapChick
* Syringe Steriliity/Shake Zwieback0 1,483 15 08/27/02 09:02 PM
by Roadkill
* Question About PESA and Sporelab bp3 1,850 4 05/13/09 08:27 AM
by RogerRabbit
* How many Quart Jars can a Sporelab syringe incoulate? Solidcell 3,147 17 01/26/06 02:38 AM
by Roadkill
* First time, LY, from Sporelab jong21 1,604 14 07/12/03 03:59 PM
by jong21
* Does Sporelab have a good Rep? shroom_daddy 1,478 3 09/17/07 08:42 PM
by Roadkill
* a good way to inoculate without seringe and only a sporeprin
( 1 2 all )		 salazare 2,311 25 05/02/03 10:42 AM
by Ekstaza
* Problems Encountered During Inoculation sillvyr 1,504 8 01/23/02 11:29 AM
by sillvyr

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
849 topic views. 24 members, 144 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.011 seconds on 14 queries.