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OfflineFYP
Stranger
Registered: 09/20/01
Posts: 1
Last seen: 22 years, 6 months
late sterilisation/inoculation
    #398864 - 09/20/01 03:22 AM (22 years, 6 months ago)

I prepared 10 jars to be inoculated, but, due to a problem, I only sterilized them and inoculated them the next day, now it's been 5 days, and still no sign of growing, is this the reason why ? did the substrate get too dry or something ?


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OfflineKosmokos
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Registered: 09/01/01
Posts: 6
Loc: U.S.A.
Last seen: 22 years, 4 months
Re: late sterilisation/inoculation [Re: FYP]
    #398873 - 09/20/01 03:39 AM (22 years, 6 months ago)

Just last week we made up a bunch of jars and "because of some problems" we waited a couple days---not thinking about mold---to sterilize and innoculate. Well, needless to say they got all moldy and we had to start all over. Rule of thumb on that one is: Sterilize the same day you make the jars. Otherwise you'll get mold forming.
Now, in your case the mold probably started to form in those 24 hours between creation and sterilization (however it should have been killled off by sterlizing, but I don't know much about mold once it forms) and that MAY have something to do with the fact that they're not colonizing; but I'm not an expert on that one. Temperature is another factor that will determine what rate tthe mycelium grows at. Give 'em another week at least 70 Fahrenheit and if you have nothing by then, I say start over.
Are you sure your spore syringes are good??

"He who's knows others is wise, he who knows himself is enlightened." ---Lao Tzu


--------------------
"He who's knows others is wise, he who knows himself is enlightened." ---Lao Tzu

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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: late sterilisation/inoculation [Re: FYP]
    #398892 - 09/20/01 04:41 AM (22 years, 6 months ago)

You ought to wait a few days,then worry.


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Anonymous

Re: late sterilisation/inoculation [Re: FYP]
    #398906 - 09/20/01 05:30 AM (22 years, 6 months ago)

And try to get those temps up to about 82.

Take a trip to the Spore Lab @:
http://www.SporeLab.com
email: getspores@sporelab.com

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Invisibleoscill8
*bondage fairy*
Registered: 02/26/01
Posts: 1,215
Re: late sterilisation/inoculation [Re: Kosmokos]
    #399036 - 09/20/01 11:13 AM (22 years, 6 months ago)

kos- many people do wait several days before innoculation, its called the control jar technique. it lets you verify your sterilization of the substrate was successful. if you have nasites growing in there before you innoc., you know you did not sterilize the jars correctly. when prepared properly, nothing will grow in them within those few days. if youre gettimg mold or anything in there before innoc., check your sterilization b/c its not working.


***Please Donate Blood. American Red Cross***


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Offlinedarkfly
LysergicExperimenter

Registered: 09/16/01
Posts: 1,169
Last seen: 8 years, 8 months
Re: late sterilisation/inoculation [Re: oscill8]
    #399092 - 09/20/01 12:01 PM (22 years, 6 months ago)

I think he let his jars sit for a day before sterilizing them.

darkfly

-- too much trippin and my soul is worn thin


--------------------
Everything Is Blue In This World. The Deepest Shade Of Mushroom Blue.

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