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Anonymous

7 Down, One to Go
    #3962855 - 03/24/05 01:55 AM (11 years, 8 months ago)

So my friend and I just recently started our first grow using PF jars. Apparently we were far from sterile enough, and 7 out of 8 jars got contamed. I'm talking cobweb, green, black, all kinds of contams. So we have this one left, 95% covered in myc. We obviously aren't going to get many mushies out of it if we just birth it into a fruiting chamber. What would happen if I mixed up some more BRF/Verm mix, (a few (7?) jars worth), and mixed this with the crumbled up myc. jar, and put it all into a casing. Would the mycelium grow throughout the casing and eventually have the same amount as if we would have crumbled 8 fully colonized jars into the casing?

If not, any ideas on how we could get the most out of our survivor?

Thanks, peace =0)


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InvisibleMrMaddHatter
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Re: 7 Down, One to Go [Re: ]
    #3962876 - 03/24/05 01:59 AM (11 years, 8 months ago)

To do what I think you're saying, you'd have to be pretty damn sterile.


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Invisiblep4kSouL
Animals Are Cool
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Re: 7 Down, One to Go [Re: MrMaddHatter]
    #3962885 - 03/24/05 02:00 AM (11 years, 8 months ago)

How did you sterilize?


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Offline88mustanggt
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Re: 7 Down, One to Go [Re: MrMaddHatter]
    #3962892 - 03/24/05 02:02 AM (11 years, 8 months ago)

you could do grain to grain, but that seems a lil advanced sence you cant get the pf jars to work. you cant jsut case it with uncolonised jars it will contam for sure.

you can just fruit that one jar and maybe you will each get a trip off of it.


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Anonymous

Re: 7 Down, One to Go [Re: p4kSouL]
    #3962909 - 03/24/05 02:05 AM (11 years, 8 months ago)

We PCd the jars for about 20 minutes.  Some things we screwed up on though:

-Didn't sterilize needle between inocs
-Accidentally squirt out some of the syringe onto a plate, then sucked it back up. ( :blush: )
-Ordered from Earth's Tongue (possible contaminated syringe from the start)


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Anonymous

Re: 7 Down, One to Go [Re: 88mustanggt]
    #3962914 - 03/24/05 02:07 AM (11 years, 8 months ago)

Quote:

88mustanggt said:
you could do grain to grain, but that seems a lil advanced sence you cant get the pf jars to work. you cant jsut case it with uncolonised jars it will contam for sure.

you can just fruit that one jar and maybe you will each get a trip off of it.




Ahh so the point of having so many jars in a casing is so the myc. can fight off contams. Gotcha. Oh well, looks like we'll just fruit this jar.


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InvisibleMrMaddHatter
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Re: 7 Down, One to Go [Re: ]
    #3962951 - 03/24/05 02:14 AM (11 years, 8 months ago)

Quote:

DirtyHippie said:
Ahh so the point of having so many jars in a casing is so the myc. can fight off contams.




I wouldn't say that.


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InvisibleMrMaddHatter
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Re: 7 Down, One to Go [Re: MrMaddHatter]
    #3962957 - 03/24/05 02:15 AM (11 years, 8 months ago)

I mean, I don't think that that is the point of having so many jars in a casing.

Having a bunch of jars in one casing is so you'll get more fruits.


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OfflineGregHimself
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Re: 7 Down, One to Go [Re: ]
    #3962959 - 03/24/05 02:16 AM (11 years, 8 months ago)

Quote:

DirtyHippie said:
-Accidentally squirt out some of the syringe onto a plate, then sucked it back up. ( :blush: )





Just from that, i am amazed you got one jar to work...try again soon, it will work well..dont you worry.


--------------------
"The key to successful cultivation is to match the skills of the cultivator with the right strain on the proper substrate under ideal environmental conditions."
Paul Stamets
Growing Gourmet and Medicinal Mushrooms(Third Edition)


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Anonymous

Re: 7 Down, One to Go [Re: MrMaddHatter]
    #3962978 - 03/24/05 02:21 AM (11 years, 8 months ago)

Quote:

MrMaddHatter said:
I mean, I don't think that that is the point of having so many jars in a casing.

Having a bunch of jars in one casing is so you'll get more fruits.




Yeah I see what you're saying...I worded that kind of wrong. I meant to say that you need all the jars in the casing (however many that may be) to be colonized so the myc. can fight contams.


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Offlinescrname
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Re: 7 Down, One to Go [Re: ]
    #3962983 - 03/24/05 02:22 AM (11 years, 8 months ago)

Quote:

DirtyHippie said:
-Accidentally squirt out some of the syringe onto a plate, then sucked it back up. ( :blush: )





Stupid move. doh!


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InvisibleMrMaddHatter
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Re: 7 Down, One to Go [Re: ]
    #3962987 - 03/24/05 02:23 AM (11 years, 8 months ago)

Quote:

DirtyHippie said:
[you need all the jars in the casing (however many that may be) to be colonized so the myc. can fight contams.




:thumbup:


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Anonymous

Re: 7 Down, One to Go [Re: GregHimself]
    #3962991 - 03/24/05 02:24 AM (11 years, 8 months ago)

Quote:

GregHimself said:
Quote:

DirtyHippie said:
-Accidentally squirt out some of the syringe onto a plate, then sucked it back up. ( :blush: )





Just from that, i am amazed you got one jar to work...try again soon, it will work well..dont you worry.




Haha, I agree.  Well at least that was my friend's screwup, not mine.  :mad:  :tongue:

We're ordering some new syringes from a Shroomery sponsor this time, and of course we will have learned from out sterility mistakes.  Thanks for the help everyone!


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InvisibleMrMaddHatter
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Re: 7 Down, One to Go [Re: ]
    #3963002 - 03/24/05 02:26 AM (11 years, 8 months ago)

Sterility is next to godliness. or something like that :tongue:


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Anonymous

Re: 7 Down, One to Go [Re: MrMaddHatter]
    #3963020 - 03/24/05 02:30 AM (11 years, 8 months ago)

Quote:

MrMaddHatter said:
Sterility is next to godliness. or something like that :tongue:




Yes!!  :smile:

"Emptiness is loneliness, and loneliness is cleanliness
And cleanliness is godliness, and god is empty just like me"

-Smashing Pumpkins - Zero


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Invisibledog
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Registered: 06/20/02
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Re: 7 Down, One to Go [Re: ]
    #3963045 - 03/24/05 02:37 AM (11 years, 8 months ago)

Quote:

We obviously aren't going to get many mushies out of it if we just birth it into a fruiting chamber. What would happen if I mixed up some more BRF/Verm mix, (a few (7?) jars worth), and mixed this with the crumbled up myc. jar, and put it all into a casing.




It would almost certainly contaminate. What you can do with an excellent chance of success is grind that PF cake up with a cheese grater and use it to spawn some pasteurized straw or dung. You'll love the results.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Anonymous

Re: 7 Down, One to Go [Re: dog]
    #3963202 - 03/24/05 03:12 AM (11 years, 8 months ago)

Intriguing. I'll have to do some research on straw and dung (of which I know nothing about). Thanks for the suggestion.

=0)


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