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OfflineSucellos
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Registered: 01/31/05
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Last seen: 10 years, 6 months
microwave sterilization
    #3961947 - 03/23/05 11:08 PM (12 years, 6 months ago)

I do not have sufficient funds to purchase a PC. I need a way to sterilize substrate and this was all I could find. My questions are.

1. What is the right time to cook it as to not dry out the substrate.
2. What would be the best substrate to use for this tek.
3. Does this actually work and has anyone had any expirience with this.


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OfflineMages
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Re: microwave sterilization [Re: Sucellos]
    #3962144 - 03/23/05 11:34 PM (12 years, 6 months ago)

General consensus is that this is not a trustworthy method of sterilization. Steaming or boiling the jars in a larger pan is more trustworthy. This is out of the original PF Tek:

Step 4. Heat the pot of water to a boil. Put the jars into the pot with the lid bands loose so that the steam can penetrate the jars quickly. The jars can sit in water but make sure boiling water can't slosh into the jars. Turn the heat down and GENTLY steam the jars at the lowest possible boil for an hour in a TIGHTLY covered pot (gas stoves are the easiest to control). A good tight fitting pot lid is essential for successful steaming.

The PF Tek can be found here: http://www.shroomery.org/index/par/23593/pag/0

If you have no alternative to microwaving, then I would go overboard on it to make sure of sterility. I.E. Whatever number of times the tek tells you to microwave it, I would double that number. It's a pain, but not nearly as much a pain as having all your jars sporting pretty green mold.


--------------------
- Mages

Ignorance killed the cat; curiosity was framed.
- C.J. Cherryh


Edited by Mages (03/23/05 11:38 PM)


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OfflineSucellos
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Re: microwave sterilization [Re: Mages]
    #3962165 - 03/23/05 11:38 PM (12 years, 6 months ago)

ok well i need to sterilize the substrate also. So will the microwave not work for that?


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OfflineMages
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Re: microwave sterilization [Re: Sucellos]
    #3962172 - 03/23/05 11:39 PM (12 years, 6 months ago)

It means the jars of substrate .


--------------------
- Mages

Ignorance killed the cat; curiosity was framed.
- C.J. Cherryh


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OfflineIGnosticAbhorI
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Re: microwave sterilization [Re: Mages]
    #3962187 - 03/23/05 11:41 PM (12 years, 6 months ago)

:smirk: :handth:


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Offlinegoofy98
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Re: microwave sterilization [Re: IGnosticAbhorI]
    #3962211 - 03/23/05 11:45 PM (12 years, 6 months ago)

actully you could justuse  :thumbup: a steamer pot, ie. crab pot (i live in mayrland).  Let the steam pot get good and steamy put the lid over it and steam for like an hour or so.  works perfectly.  Do cover the lids with foil though and let cool in the pot.  The jars will be very hot.


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OfflineChunkster
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Registered: 01/28/05
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Re: microwave sterilization [Re: goofy98]
    #3962575 - 03/24/05 12:57 AM (12 years, 6 months ago)

So far the microwave tek has not steered me wrong; 6 jars colonizing and none of them show any signs of contams.


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InvisibleMagashM
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Re: microwave sterilization [Re: Sucellos]
    #3963503 - 03/24/05 04:42 AM (12 years, 6 months ago)

You know for the pf tek I use to just boil jars for a hour when I was starting out and didn't have a pc yet.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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