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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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Offlinejtk
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getting more from a syringe?
    #3960577 - 03/23/05 06:05 PM (11 years, 8 months ago)

I just used all of a 10cc syringe. There are some globs of visible spores left in the bottom. I was wondering if i could boil some water in the microwave with saranwrap over it. Then wait for it to cool, and suck some of it up into the syringe. Would this work without getting contams into the syringe?


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Offlinefallingaway24
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Re: getting more from a syringe? [Re: jtk]
    #3960586 - 03/23/05 06:06 PM (11 years, 8 months ago)

ya, thats basicaly how you would do a liquid culture. so i don't see why it wouldn't work


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Offlinejtk
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Re: getting more from a syringe? [Re: fallingaway24]
    #3960595 - 03/23/05 06:07 PM (11 years, 8 months ago)

how long should the water boil for?


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Invisibleagar
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Re: getting more from a syringe? [Re: jtk]
    #3960641 - 03/23/05 06:14 PM (11 years, 8 months ago)

Might work fine, if all is done very sterile.

Best way to get more out of a syringe - is to prepare a liquid culture jar (pint or quart), then inject 1 or 2 ml into it.

Result - if done right - is enouhg to fill numerious syringes with live culture. Which - colonizes - VERY QUICKLY.


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OfflineBladeLSD
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Re: getting more from a syringe? [Re: jtk]
    #3960702 - 03/23/05 06:26 PM (11 years, 8 months ago)

actually that would work and you wouldnt have to make a liquid culture just stretch syringe, and use the last of it for a lliquid culture, should work fine a foaf just did it with malabars


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OfflineMages
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Re: getting more from a syringe? [Re: jtk]
    #3960726 - 03/23/05 06:33 PM (11 years, 8 months ago)

I've done that and had no problems - just boil the water REAL well, and make sure it's cool - also flame the needle before sucking up more water just like you would before inoculation. Done right, there's not much chance of contamination.

This is a little chancier, but it worked for me: don't fill the jar full before you sterilize it - leave about an inch of air at the top. Then that air will sterilize, too. Fill the syringe about half-way with water, then suck in a little of the sterilized air. Then shake the shit out of the syringe. Then shoot the air out of the needle, re-flame and fill the needle the rest of the way with water.


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- Mages

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- C.J. Cherryh


Edited by Mages (03/23/05 06:39 PM)


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Amazon Shop for: ½ Pint Jars, 10cc Syringe

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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