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 ShroomNoob03
Seeker of Knowledge
Registered: 09/03/03
Posts: 392
Loc: The Depths of My Psyche
Last seen: 13 years, 8 days
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 If you wanted to convert a room into a sterile lab, how would you do it? 
#3953971 - 03/22/05 02:02 PM (19 years, 30 days ago) |
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It would make my life so much easier if I had one room devoted to all of my inoculations, extractions, everything. I?m seriously thinking about doing this in the future and I was just looking for some feedback in case I ever decide to do something like this.
Well, to start things off?
What objects would be a necessity?
A Sink
Air Filter (over the air vents, I guess)
Flow Hood
Humidity/Temp Monitor (whatever you call em)
What other supplies would I need?
Glassware (Petri Dishes, Beakers, Flasks, Etc.)
Various Instruments (I dunno, all those little gadgets)
Lab Coat (Hah, of course)
Safety Equipment
I would probably never be able to come up with the funds for anything serious, I?m not looking to create a fully stocked lab, just an environment I know will be as sterile as I can afford.
Thanks for reading,
ShroomNoob03
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In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.
-Carl Sagan
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 MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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 Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
#3954003 - 03/22/05 02:07 PM (19 years, 30 days ago) |
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Looks like you got the basic idea of it.
Only thing I can think of thats not there is = no carpet on floor. But you probably already knew that 
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kronnyQ
SuperstudExtraordinaire
Registered: 07/22/04
Posts: 2,488
Loc: Anytown USA
Last seen: 9 days, 6 hours
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: MrMaddHatter]
#3954042 - 03/22/05 02:17 PM (19 years, 30 days ago) |
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I've done this...unfortunately it only worked for me for a couple of months because I made the lab on carpet and too much moisture built up under the lab and created tons of mildew. It has since dried out quite a bit and I still use it for prints and putting together casings.
If you do it try not to do it on a carpet, if you have carpet try to keep the inside as dry as possible.
Also, I mounted a 80mm PC fan for exhaust on one of the walls....with fresh air coming in, old air going out and a good HEPA blasting you shouldn't even need a flow hood. I recently made 7 casings in my lab in front of hepa, not 1 contam.
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djsage420
aka DJQBNSIS
Registered: 02/15/05
Posts: 869
Loc: Panhandle
Last seen: 18 years, 1 month
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: MrMaddHatter]
#3954044 - 03/22/05 02:17 PM (19 years, 30 days ago) |
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a entry room is allways nice ceeps a rush of non-sterile air from coming in The Mushroom Cultivator has a nice chapter on this subject
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If you put your head to the grass, you can hear it growing
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
#3954088 - 03/22/05 02:30 PM (19 years, 29 days ago) |
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I'd cover the walls, ceiling and floor with Visqueen(plastic).
I'd make a clean room door with the Visqueen...a double door would even be better.
I'd use a Simco Aerostat XC Ionizer in the room...then wipe everything down on a regular basis.
I'd block the heating vents...or filter it with some kind of hepa filter.
Then use the flow hood for added protection.
And a nice glove box.
then all the other little extra's you mentioned.
The lab coat is a must...don't forget the tie!~
lolzz
take care!~
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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ShroomNoob03
Seeker of Knowledge
Registered: 09/03/03
Posts: 392
Loc: The Depths of My Psyche
Last seen: 13 years, 8 days
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: djsage420]
#3955408 - 03/22/05 06:42 PM (19 years, 29 days ago) |
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Quote:
djsage420 said:
a entry room is allways nice ceeps a rush of non-sterile air from coming in The Mushroom Cultivator has a nice chapter on this subject
Are you refering to a book or something online?
Thanks,
ShroomNoob03
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In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.
-Carl Sagan
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kronnyQ
SuperstudExtraordinaire
Registered: 07/22/04
Posts: 2,488
Loc: Anytown USA
Last seen: 9 days, 6 hours
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
#3955590 - 03/22/05 07:27 PM (19 years, 29 days ago) |
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I think he's refering to making a separate entry room on your lab.
I did this but I had to chop it off because I needed the space.
It definitely helps a lot, plus you can use it as a changing and self-sterilization room.
Just buy about 50ft of plastic, 2 rolls of duct tape, razor blade, staple gun and get buildin!
Edited by kronnyQ (03/22/05 07:29 PM)
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derx
who run it
Registered: 05/29/03
Posts: 2,459
Loc: dx/dt
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: kronnyQ]
#3955600 - 03/22/05 07:29 PM (19 years, 29 days ago) |
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yes he's referring to a book. "the mushroom cultivator" - paul stamets
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better living through chemistry
OVERGROW the government!!
it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.
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ShroomNoob03
Seeker of Knowledge
Registered: 09/03/03
Posts: 392
Loc: The Depths of My Psyche
Last seen: 13 years, 8 days
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: kronnyQ]
#3955609 - 03/22/05 07:31 PM (19 years, 29 days ago) |
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No, he was refering to a "nice chapter" in The Mushroom Cultivator. Is The Mushroom Cultivator a book or an online source.
ShroomNoob03
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In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.
-Carl Sagan
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Smushroom
Avid Learner
Registered: 02/02/05
Posts: 2,806
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
#3957125 - 03/22/05 10:53 PM (19 years, 29 days ago) |
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Book by Paul Stamets
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Fluxburn
.
Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA 
Last seen: 13 years, 4 months
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: Smushroom]
#3957538 - 03/23/05 12:44 AM (19 years, 29 days ago) |
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--------------------
ABSTRACT ART (Mine) http://nathanbelomy.com
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: Fluxburn]
#3957721 - 03/23/05 01:37 AM (19 years, 29 days ago) |
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The Bible of musrhoom cultivation.
Making clean rooms is fun!
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