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pleco
Astralnaut
Registered: 03/08/05
Posts: 203
Last seen: 16 years, 9 months
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OMG, germs do exist!
#3957335 - 03/22/05 11:39 PM (19 years, 30 days ago) |
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Hi guys... last Friday night 12 jars were "knocked up". Five are PESA, another five are TSSS. The last two each have 10cc's of Brita, one PESA one TSSS.
Besides one exception all jars were prepared the same way. The only difference is that the TSSS needle was heated under a lighter until water shot out. The PESA was used as-is.
The reason you're hearing about all of this is that the PESA is showing no signs of life. I think the TSS is doing fine for 5 days, or maybe not, but it's alive.
So the lesson is sterylize everything... I know you've heard it before but it's actually true. Really...
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p4kSouL
Animals Are Cool
Registered: 01/13/05
Posts: 3,666
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Re: OMG, germs do exist! [Re: pleco]
#3957353 - 03/22/05 11:43 PM (19 years, 30 days ago) |
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Wait longer. If you did everything right they should come.
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pleco
Astralnaut
Registered: 03/08/05
Posts: 203
Last seen: 16 years, 9 months
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Re: OMG, germs do exist! [Re: p4kSouL]
#3957377 - 03/22/05 11:48 PM (19 years, 30 days ago) |
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Hope so Soul! There's only one fruiting chamber anyway so maybe the timing is better this way.
BTW, I know PF Jars like to be around something like 70-85 degrees... I'm guessing it's about 77-78 where they are. But when you touch the jars it's actaully pretty cold. Would putting them in a Rubbermaid box be a good idea?
Edited by pleco (03/22/05 11:48 PM)
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: OMG, germs do exist! [Re: pleco]
#3957386 - 03/22/05 11:53 PM (19 years, 30 days ago) |
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without flaming the needle, you increase your risk of contams
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Dem_Bones
Strangler
Registered: 02/12/05
Posts: 307
Loc: Oklahoma
Last seen: 2 years, 19 days
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Re: OMG, germs do exist! [Re: pleco]
#3957406 - 03/22/05 11:59 PM (19 years, 30 days ago) |
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did you put 12c in the last 2 jars from 3 diff strans?
hey by the way what makes a stran a stran is a diffrance. if all teh tryes hached teh same time grow teh same and fruted the same i wouls say you got riped off
-------------------- At the bottom of this page is a search bar to search all the post and threads on the forum so you can see all the other 500 people who asked the same thing.use the top search too it has shroomery approved FAQs USE IT YOU CAN DO IT !
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p4kSouL
Animals Are Cool
Registered: 01/13/05
Posts: 3,666
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Re: OMG, germs do exist! [Re: pleco]
#3957409 - 03/23/05 12:00 AM (19 years, 30 days ago) |
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Quote:
pleco said: Would putting them in a Rubbermaid box be a good idea?
yes that is a good idea. Helps keep the air contams out. Check out my little incubator (lid is usually on)-
Contam FREE!
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 5 days, 23 hours
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Re: OMG, germs do exist! [Re: pleco]
#3957639 - 03/23/05 01:13 AM (19 years, 30 days ago) |
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>last Friday night 12 jars were "knocked up"
Can you say inoculated?
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: OMG, germs do exist! [Re: Anno]
#3957691 - 03/23/05 01:26 AM (19 years, 30 days ago) |
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Quote:
Anno said: >last Friday night 12 jars were "knocked up"
Can you say inoculated?
Maybe he used "penis" syringe & sperm solution to actualy knock them up -->(joke).
BTW, if anyone doesn't know it -->ANNO<-- hates the term "knocked up".
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pleco
Astralnaut
Registered: 03/08/05
Posts: 203
Last seen: 16 years, 9 months
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Re: OMG, germs do exist! [Re: p4kSouL]
#3957716 - 03/23/05 01:36 AM (19 years, 30 days ago) |
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Ahh, cool man. I'll incubate them now.
I see you're trying pint-sized jars, too. How long has it been since you them?
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pleco
Astralnaut
Registered: 03/08/05
Posts: 203
Last seen: 16 years, 9 months
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Re: OMG, germs do exist! [Re: agar]
#3957730 - 03/23/05 01:40 AM (19 years, 30 days ago) |
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Quote:
agar said:
Quote:
Anno said: >last Friday night 12 jars were "knocked up"
Can you say inoculated?
Maybe he used "penis" syringe & sperm solution to actualy knock them up -->(joke).
BTW, if anyone doesn't know it -->ANNO<-- hates the term "knocked up".
Oh, really? Lol... Sorry Anno, I'll refrain from using that term. They have the same meaning though, kinda: "Inoculate - The introduction of a microbe into surroundings capable of supporting its growth." Sperm just isn't microbial, is it? What the hell is sperm anyway?
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: OMG, germs do exist! [Re: Anno]
#3957732 - 03/23/05 01:42 AM (19 years, 30 days ago) |
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Quote:
Anno said:
>last Friday night 12 jars were "knocked up"
Can you say inoculated?
in?oc?u?lat?ed
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 5 days, 23 hours
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Re: OMG, germs do exist! [Re: pleco]
#3957734 - 03/23/05 01:42 AM (19 years, 30 days ago) |
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Re: OMG, germs do exist! [Re: Anno]
#3957736 - 03/23/05 01:44 AM (19 years, 30 days ago) |
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I can say it and I'm a little tipsy.
Inoculated
Quote:
agar said:
Quote:
Anno said: -->ANNO<--
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: OMG, germs do exist! [Re: pleco]
#3957739 - 03/23/05 01:46 AM (19 years, 30 days ago) |
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>>>>>>What the hell is sperm anyway?<<<<<
Sperm is what "knocks up" womem, without contaceptive use.
Glad - you have a sence of humour. Was only a "joke", not a flame.
I use the abreviated term "innoc".... or "innoc'ed".
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 5 days, 23 hours
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Re: OMG, germs do exist! [Re: agar]
#3957741 - 03/23/05 01:47 AM (19 years, 30 days ago) |
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>innoc'ed
grrrr.....
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 5 days, 23 hours
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Re: OMG, germs do exist! [Re: agar]
#3957743 - 03/23/05 01:48 AM (19 years, 30 days ago) |
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I hate all those nn too.....
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Re: OMG, germs do exist! [Re: pleco]
#3957745 - 03/23/05 01:48 AM (19 years, 30 days ago) |
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Quote:
pleco said: What the hell is sperm anyway?
Are you serious?
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: OMG, germs do exist! [Re: Anno]
#3957746 - 03/23/05 01:48 AM (19 years, 30 days ago) |
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lmfaooo @ you
too funny!~
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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pleco
Astralnaut
Registered: 03/08/05
Posts: 203
Last seen: 16 years, 9 months
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Re: OMG, germs do exist! [Re: agar]
#3957747 - 03/23/05 01:50 AM (19 years, 30 days ago) |
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Oh boy, there I go opening another can of sperm.
See agar, I do have a sense of humor... it just doesn't read well
Edited by pleco (03/23/05 01:52 AM)
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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Re: OMG, germs do exist! [Re: Anno]
#3957752 - 03/23/05 01:51 AM (19 years, 30 days ago) |
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Anno is right. Its good to keep up correct terminology. Very professional.
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