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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar

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Offlinepleco
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Registered: 03/09/05
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OMG, germs do exist!
    #3957335 - 03/23/05 01:39 AM (11 years, 8 months ago)

Hi guys... last Friday night 12 jars were "knocked up". Five are PESA, another five are TSSS. The last two each have 10cc's of Brita, one PESA one TSSS.

Besides one exception all jars were prepared the same way. The only difference is that the TSSS needle was heated under a lighter until water shot out. The PESA was used as-is.

The reason you're hearing about all of this is that the PESA is showing no signs of life. I think the TSS is doing fine for 5 days, or maybe not, but it's alive.

So the lesson is sterylize everything... I know you've heard it before but it's actually true. Really...


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Invisiblep4kSouL
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Re: OMG, germs do exist! [Re: pleco]
    #3957353 - 03/23/05 01:43 AM (11 years, 8 months ago)

Wait longer. If you did everything right they should come.


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Offlinepleco
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Re: OMG, germs do exist! [Re: p4kSouL]
    #3957377 - 03/23/05 01:48 AM (11 years, 8 months ago)

Hope so Soul! There's only one fruiting chamber anyway so maybe the timing is better this way.

BTW, I know PF Jars like to be around something like 70-85 degrees... I'm guessing it's about 77-78 where they are. But when you touch the jars it's actaully pretty cold. Would putting them in a Rubbermaid box be a good idea?


Edited by pleco (03/23/05 01:48 AM)


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InvisiblePrisoner#1M
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Re: OMG, germs do exist! [Re: pleco]
    #3957386 - 03/23/05 01:53 AM (11 years, 8 months ago)

without flaming the needle, you increase your risk of contams


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OfflineDem_Bones
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Re: OMG, germs do exist! [Re: pleco]
    #3957406 - 03/23/05 01:59 AM (11 years, 8 months ago)

did you put 12c in the last 2 jars from 3 diff strans?

hey by the way what makes a stran a stran is a diffrance. if all teh tryes hached teh same time grow teh same and fruted the same i wouls say you got riped off


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Invisiblep4kSouL
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Re: OMG, germs do exist! [Re: pleco]
    #3957409 - 03/23/05 02:00 AM (11 years, 8 months ago)

Quote:

pleco said:
Would putting them in a Rubbermaid box be a good idea?




yes that is a good idea. Helps keep the air contams out. Check out my little incubator (lid is usually on)-


Contam FREE! :smile:


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OfflineAnnoA
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Re: OMG, germs do exist! [Re: pleco]
    #3957639 - 03/23/05 03:13 AM (11 years, 8 months ago)

>last Friday night 12 jars were "knocked up"

Can you say inoculated?


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Invisibleagar
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Re: OMG, germs do exist! [Re: Anno]
    #3957691 - 03/23/05 03:26 AM (11 years, 8 months ago)

Quote:

Anno said:
>last Friday night 12 jars were "knocked up"

Can you say inoculated?




Maybe he used "penis" syringe & sperm solution to actualy knock them up -->(joke).

BTW, if anyone doesn't know it -->:bow:ANNO:bow:<-- hates the term "knocked up". :wink:


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Offlinepleco
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Re: OMG, germs do exist! [Re: p4kSouL]
    #3957716 - 03/23/05 03:36 AM (11 years, 8 months ago)

Ahh, cool man. I'll incubate them now.

I see you're trying pint-sized jars, too. How long has it been since you :syringe: them?  :tongue:


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Offlinepleco
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Re: OMG, germs do exist! [Re: agar]
    #3957730 - 03/23/05 03:40 AM (11 years, 8 months ago)

Quote:

agar said:
Quote:

Anno said:
>last Friday night 12 jars were "knocked up"

Can you say inoculated?




Maybe he used "penis" syringe & sperm solution to actualy knock them up -->(joke).

BTW, if anyone doesn't know it -->:bow:ANNO:bow:<-- hates the term "knocked up". :wink:




Oh, really?  Lol... Sorry Anno, I'll refrain from using that term. They have the same meaning though, kinda: "Inoculate - The introduction of a microbe into surroundings capable of supporting its growth." Sperm just isn't microbial, is it? What the hell is sperm anyway?


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InvisibleRoadkill
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Re: OMG, germs do exist! [Re: Anno]
    #3957732 - 03/23/05 03:42 AM (11 years, 8 months ago)

Quote:

Anno said:

>last Friday night 12 jars were "knocked up"

Can you say inoculated?




in?oc?u?lat?ed

:smile:


--------------------
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Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineAnnoA
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Re: OMG, germs do exist! [Re: pleco]
    #3957734 - 03/23/05 03:42 AM (11 years, 8 months ago)



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InvisibleMrMaddHatter
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Re: OMG, germs do exist! [Re: Anno]
    #3957736 - 03/23/05 03:44 AM (11 years, 8 months ago)

I can say it and I'm a little tipsy.

Inoculated :tongue:

Quote:

agar said:
Quote:

Anno said:
-->:bow:ANNO:bow:<--




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Invisibleagar
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Re: OMG, germs do exist! [Re: pleco]
    #3957739 - 03/23/05 03:46 AM (11 years, 8 months ago)

>>>>>>What the hell is sperm anyway?<<<<<

Sperm is what "knocks up" womem, without contaceptive use.

Glad - you have a sence of humour. Was only a "joke", not a flame.

I use the abreviated term "innoc".... or "innoc'ed".


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OfflineAnnoA
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Re: OMG, germs do exist! [Re: agar]
    #3957741 - 03/23/05 03:47 AM (11 years, 8 months ago)

>innoc'ed

grrrr.....


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OfflineAnnoA
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Re: OMG, germs do exist! [Re: agar]
    #3957743 - 03/23/05 03:48 AM (11 years, 8 months ago)

I hate all those nn too.....  :wink:


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InvisibleMrMaddHatter
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Re: OMG, germs do exist! [Re: pleco]
    #3957745 - 03/23/05 03:48 AM (11 years, 8 months ago)

Quote:

pleco said:
What the hell is sperm anyway?




Are you serious? :crazy2:


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InvisibleRoadkill
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Re: OMG, germs do exist! [Re: Anno]
    #3957746 - 03/23/05 03:48 AM (11 years, 8 months ago)

lmfaooo @ you

too funny!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinepleco
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Registered: 03/09/05
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Re: OMG, germs do exist! [Re: agar]
    #3957747 - 03/23/05 03:50 AM (11 years, 8 months ago)

Oh boy, there I go opening another can of sperm.

See agar, I do have a sense of humor... it just doesn't read well  :crazy2:


Edited by pleco (03/23/05 03:52 AM)


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InvisibleMrMaddHatter
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Re: OMG, germs do exist! [Re: Anno]
    #3957752 - 03/23/05 03:51 AM (11 years, 8 months ago)

Anno is right. Its good to keep up correct terminology. Very professional. :thumbup:


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar

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