Home | Community | Message Board


The Spore Depot
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy CBD, Compare CBD   Amazon HEPA Filter, Lab Glassware, Laminar Flow Hood, Paul Stamets, Petri Dish

Jump to first unread post. Pages: 1
OfflineShroomNoob03
Seeker of Knowledge
Male
Registered: 09/03/03
Posts: 392
Loc: The Depths of My Psyche
Last seen: 6 years, 3 months
If you wanted to convert a room into a sterile lab, how would you do it?
    #3953971 - 03/22/05 04:02 PM (12 years, 4 months ago)

It would make my life so much easier if I had one room devoted to all of my inoculations, extractions, everything. I?m seriously thinking about doing this in the future and I was just looking for some feedback in case I ever decide to do something like this.

Well, to start things off?

What objects would be a necessity?

A Sink
Air Filter (over the air vents, I guess)
Flow Hood
Humidity/Temp Monitor (whatever you call em)

What other supplies would I need?

Glassware (Petri Dishes, Beakers, Flasks, Etc.)
Various Instruments (I dunno, all those little gadgets)
Lab Coat (Hah, of course)
Safety Equipment

I would probably never be able to come up with the funds for anything serious, I?m not looking to create a fully stocked lab, just an environment I know will be as sterile as I can afford.

Thanks for reading,
ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,418
Loc: Everywhere
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
    #3954003 - 03/22/05 04:07 PM (12 years, 4 months ago)

Looks like you got the basic idea of it.

Only thing I can think of thats not there is = no carpet on floor. But you probably already knew that :tongue:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinekronnyQ
SuperstudExtraordinaire
 User Gallery

Registered: 07/23/04
Posts: 2,488
Loc: Anytown USA
Last seen: 1 year, 13 days
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: MrMaddHatter]
    #3954042 - 03/22/05 04:17 PM (12 years, 4 months ago)

I've done this...unfortunately it only worked for me for a couple of months because I made the lab on carpet and too much moisture built up under the lab and created tons of mildew. It has since dried out quite a bit and I still use it for prints and putting together casings.

If you do it try not to do it on a carpet, if you have carpet try to keep the inside as dry as possible.

Also, I mounted a 80mm PC fan for exhaust on one of the walls....with fresh air coming in, old air going out and a good HEPA blasting you shouldn't even need a flow hood. I recently made 7 casings in my lab in front of hepa, not 1 contam.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedjsage420
aka DJQBNSIS
 User Gallery

Registered: 02/15/05
Posts: 869
Loc: Panhandle
Last seen: 11 years, 4 months
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: MrMaddHatter]
    #3954044 - 03/22/05 04:17 PM (12 years, 4 months ago)

a entry room is allways nice ceeps a rush of non-sterile air from coming in The Mushroom Cultivator has a nice chapter on this subject


--------------------
If you put your head to the grass, you can hear it growing


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
    #3954088 - 03/22/05 04:30 PM (12 years, 4 months ago)

I'd cover the walls, ceiling and floor with Visqueen(plastic).

I'd make a clean room door with the Visqueen...a double door would even be better.

I'd use a Simco Aerostat XC Ionizer in the room...then wipe everything down on a regular basis.

I'd block the heating vents...or filter it with some kind of hepa filter.

Then use the flow hood for added protection.

And a nice glove box.

then all the other little extra's you mentioned.

The lab coat is a must...don't forget the tie!~


lolzz

take care!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShroomNoob03
Seeker of Knowledge
Male
Registered: 09/03/03
Posts: 392
Loc: The Depths of My Psyche
Last seen: 6 years, 3 months
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: djsage420]
    #3955408 - 03/22/05 08:42 PM (12 years, 4 months ago)

Quote:

djsage420 said:
a entry room is allways nice ceeps a rush of non-sterile air from coming in The Mushroom Cultivator has a nice chapter on this subject



Are you refering to a book or something online?

Thanks,
ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinekronnyQ
SuperstudExtraordinaire
 User Gallery

Registered: 07/23/04
Posts: 2,488
Loc: Anytown USA
Last seen: 1 year, 13 days
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
    #3955590 - 03/22/05 09:27 PM (12 years, 4 months ago)

I think he's refering to making a separate entry room on your lab.

I did this but I had to chop it off because I needed the space.

It definitely helps a lot, plus you can use it as a changing and self-sterilization room.

Just buy about 50ft of plastic, 2 rolls of duct tape, razor blade, staple gun and get buildin!


Edited by kronnyQ (03/22/05 09:29 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblederx
who run it
 User Gallery

Registered: 05/30/03
Posts: 2,459
Loc: dx/dt
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: kronnyQ]
    #3955600 - 03/22/05 09:29 PM (12 years, 4 months ago)

yes he's referring to a book. "the mushroom cultivator" - paul stamets


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShroomNoob03
Seeker of Knowledge
Male
Registered: 09/03/03
Posts: 392
Loc: The Depths of My Psyche
Last seen: 6 years, 3 months
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: kronnyQ]
    #3955609 - 03/22/05 09:31 PM (12 years, 4 months ago)

No, he was refering to a "nice chapter" in The Mushroom Cultivator. Is The Mushroom Cultivator a book or an online source.

ShroomNoob03


--------------------
In science it often happens that scientists say, 'You know that's a really good argument; my position is mistaken,' and then they would actually change their minds and you never hear that old view from them again. They really do it. It doesn't happen as often as it should, because scientists are human and change is sometimes painful. But it happens every day. I cannot recall the last time something like that happened in politics or religion.

-Carl Sagan


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSmushroom
Avid Learner
Registered: 02/02/05
Posts: 2,803
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: ShroomNoob03]
    #3957125 - 03/23/05 12:53 AM (12 years, 4 months ago)

Book by Paul Stamets


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFluxburn
.
 User Gallery

Registered: 10/23/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 6 years, 8 months
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: Smushroom]
    #3957538 - 03/23/05 02:44 AM (12 years, 4 months ago)

You mean this one?









--------------------
ABSTRACT ART (Mine) http://nathanbelomy.com


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,418
Loc: Everywhere
Re: If you wanted to convert a room into a sterile lab, how would you do it? [Re: Fluxburn]
    #3957721 - 03/23/05 03:37 AM (12 years, 4 months ago)

The Bible of musrhoom cultivation.

Making clean rooms is fun!


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy CBD, Compare CBD   Amazon HEPA Filter, Lab Glassware, Laminar Flow Hood, Paul Stamets, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* My Sterile Lab (pics) & First agar work
( 1 2 all )
ThailandBound 3,291 26 12/23/14 11:36 AM
by wowimflabbergasted
* Sterile lab in a hotel room?
( 1 2 all )
feedbox 1,914 27 07/27/08 12:06 PM
by Psuper
* Sterile Lab BPJohnny 859 8 03/29/06 12:46 AM
by Tippinthru
* I hate carpet cause it sucks goofy98 1,334 10 04/01/05 01:46 PM
by goofy98
* Better to sterilize syringe with flame or alcohol?
( 1 2 all )
HUBSonDUBS 3,779 24 07/28/09 08:09 PM
by anonjon
* Sterilizing a non-autoclavable petri dish??
( 1 2 all )
psilosibling 4,409 21 05/03/07 07:34 AM
by mushietek
* ...AGAR Help!!!Sterilizing poo....
( 1 2 all )
FungusMan 2,810 21 01/31/06 09:02 AM
by RogerRabbit
* what's the difference between a sterilizer and a pressure cooker? xhooliganx 3,442 6 04/01/06 12:03 PM
by mogur

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,015 topic views. 13 members, 90 guests and 38 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
FreeSpores.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.003 seconds on 16 queries.