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InvisiblePsychoslut
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Death at 106f is bull shit
    #3943841 - 03/20/05 11:56 AM (19 years, 12 days ago)

My fish tank heater shorted out, so I have to use a heat blanket. I put 7 freshly spawned jars in my incubator, and had 14 about 40 percent already in. I set the blanket on high to pre-heat it, and forgot to back it off. The jars were at 120 degrees f for 6 days, and the new ones are growing as normal, and nothing has happend to the ones at 40 percent.

who came up with the 106 thing stamets?


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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OfflineIGnosticAbhorI
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3943857 - 03/20/05 11:59 AM (19 years, 12 days ago)

It's 96F and 106F for spores...

And i know that dried out myc. can be put it statis and lost for a long time...Not so sure about heat though, it the metal was Burning ass hot then it really was 120F...if not, then ur themometer was wrong
:shrug:

-Gnostic

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OfflineIGnosticAbhorI
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Re: Death at 106f is bull shit [Re: IGnosticAbhorI]
    #3943864 - 03/20/05 11:59 AM (19 years, 12 days ago)

And Stamet...Who else :grin:

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InvisiblePsychoslut
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Re: Death at 106f is bull shit [Re: IGnosticAbhorI]
    #3943870 - 03/20/05 12:01 PM (19 years, 12 days ago)

No man, my thermometer is fine. I could tell just by touching them they was burning up.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Offlinesludge
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3943881 - 03/20/05 12:04 PM (19 years, 12 days ago)

so are you going to start incubating them at a 120


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be

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InvisiblePsychoslut
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Re: Death at 106f is bull shit [Re: sludge]
    #3943884 - 03/20/05 12:06 PM (19 years, 12 days ago)

No no, I incubate my jars at 83 degrees. 120 is way too hot, and they didnt grow faster at that temp, but they did grow a little.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Offlinesludge
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3943891 - 03/20/05 12:10 PM (19 years, 12 days ago)

ya i know i was just fucking with you sounds like luck is on your side i dont know the facts of thermal death just what everyone says but i will tell you this 120 dos not sound comfortable :whoa:


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be

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OfflineMages
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3943894 - 03/20/05 12:11 PM (19 years, 12 days ago)

Good questions. Yeah, Stamets & Chilton say (p. 197 in the Growing Parameters for Various Mushroom Species section):
Substrate Temperature:84-86 F. Thermal death limits have been established at 106 F.

Check it out & see if they grow through. I'd like to know. I also wonder (if death below 120 doesn't happen) if those higher ranges might promote contams.


--------------------
- Mages

Ignorance killed the cat; curiosity was framed.
- C.J. Cherryh

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InvisiblePrisoner#1
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Re: Death at 106f is bull shit [Re: IGnosticAbhorI]
    #3943897 - 03/20/05 12:12 PM (19 years, 12 days ago)

Quote:

IGnosticAbhorI said:
It's 96F and 106F for spores...





spores can withstand more heat than 106F

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OfflineIGnosticAbhorI
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Re: Death at 106f is bull shit [Re: Prisoner#1]
    #3943912 - 03/20/05 12:18 PM (19 years, 12 days ago)

I know pris...

"Thermal death limits have been established at 106 F"

Doesn't mean ALWAYS :smirk:
Just most of the time

-Gnostic

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Offlinescatmanrav
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Re: Death at 106f is bull shit [Re: IGnosticAbhorI]
    #3943927 - 03/20/05 12:22 PM (19 years, 12 days ago)

Quote:

IGnosticAbhorI said:
I know pris...

"Thermal death limits have been established at 106 F"

Doesn't mean ALWAYS :smirk:
Just most of the time

-Gnostic




No, your thinking of mycelium. Death for spores happens above 160 degrees. Thermal death limits for myc have been extablished at 106 F. As you said though, doesnt mean always..it seems it held up in this case.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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OfflineIGnosticAbhorI
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Re: Death at 106f is bull shit [Re: scatmanrav]
    #3943932 - 03/20/05 12:23 PM (19 years, 12 days ago)

Hm...:thumbup:

Didn't know that, thankx Scat :grin:

-Gnostic

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OfflineMages
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Re: Death at 106f is bull shit [Re: Prisoner#1]
    #3943951 - 03/20/05 12:29 PM (19 years, 12 days ago)

Even if the heat didn't directly kill the myc., I can think of a whole bunch of other high temp problems that might eventually cause death - favoring some contams over the mycellium, high production of CO2, and high production of mycellial piss coming to mind right off. It does make me wonder how that thermal death limit was originally tested, though. Agar plates, maybe?


--------------------
- Mages

Ignorance killed the cat; curiosity was framed.
- C.J. Cherryh

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OfflineIGnosticAbhorI
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Re: Death at 106f is bull shit [Re: Mages]
    #3944019 - 03/20/05 12:46 PM (19 years, 12 days ago)

Myc. piss, ive never had it but what Exactly is it? Byproducts of myc ='s ?...

And what are some factors that would cause it
:grin:

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OfflineMushroomFriend
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3944093 - 03/20/05 01:13 PM (19 years, 12 days ago)

Once I incubated straw with B+ spawn , temps raised up to 110F.
50% of it died, maybe it didnt get THAT hot everywhere.

Doesnt matter if myc. lives or dies at 110F, one should avoid it anyway.

MF


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OfflineEnivid
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Re: Death at 106f is bull shit [Re: MushroomFriend]
    #3944228 - 03/20/05 01:50 PM (19 years, 12 days ago)

My incu chamber seems to be fluctuating from low 80's to high 80's--81-88...thought that it was a cause for concern until I saw this post. Not that I wouldn't like it stable but is now less cause for concern.

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OfflineMushroomFriend
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3944266 - 03/20/05 01:57 PM (19 years, 12 days ago)

no concerns just takes a little longer then a steady 86F


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OfflineLanceArmstrong
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Re: Death at 106f is bull shit [Re: MushroomFriend]
    #3944306 - 03/20/05 02:03 PM (19 years, 12 days ago)

Another myth that needs to die is that jars need to be cooled to room temperature after PCing. I inoculated a bunch that were warm to the touch (~110-120 F) with absolutely no problems.

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OfflineMages
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Re: Death at 106f is bull shit [Re: LanceArmstrong]
    #3944356 - 03/20/05 02:13 PM (19 years, 12 days ago)

Quote:

LanceArmstrong said:
Another myth that needs to die is that jars need to be cooled to room temperature after PCing. I inoculated a bunch that were warm to the touch (~110-120 F) with absolutely no problems.




If you're new, and read this, let it cool anyway. You MIGHT get away with it, but I once had reason to dump the substrate out of jars when they were just warm to the touch, and the interior substrate was still hot hot hot.


--------------------
- Mages

Ignorance killed the cat; curiosity was framed.
- C.J. Cherryh

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Offline_Lucid_
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Re: Death at 106f is bull shit [Re: LanceArmstrong]
    #3944439 - 03/20/05 02:27 PM (19 years, 12 days ago)

well, i had just changed my own incubator setup over last month, when i did so my fishtank heater became a lot more efficiant. where i was using something similar to a "heat bomb" (fishtank heater in a sealed container in your incubator), which needed to be maxed out to get the 84-86 F temps, when i switched over to a TiT setup i forgot to lower the temp of the heater, and my thermometer didnt give me very accurate temps because its one of those digital ones with an external probe, the trick is let it touch the bottom so it can sense what your jars are sitting on. The problem was my dangling thermometer just read the air temp in the incubator, but the bottom of my jars were getting cooked and dried. i noticed this in time and some of the deeper mycelium must have servived and sprang back, but some of it was definitly killed off by the heat, and it couldnt have been much over 96, because thats as far as my heater goes to.


--------------------
The believer is happy, the doubter is wise
- Hungarian proverb

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OfflineXTCollection
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Re: Death at 106f is bull shit [Re: _Lucid_]
    #3944478 - 03/20/05 02:34 PM (19 years, 12 days ago)

Im thinking what mushroom friend said...

Inside of the cakes never reached above 105 or somewhere around there.

120 is to hot for most living organisms, they would die if given enough time or thorough heating.

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InvisibleDexter_Morgan
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Re: Death at 106f is bull shit [Re: _Lucid_]
    #3944543 - 03/20/05 02:44 PM (19 years, 12 days ago)

I can speak for a fact that not letting the jars cool before inoculating will kill spores. Or i had dud spores.

There is still NO reason to tell people that it is "myth". A hot core temperature will kill spores, even if your jars are only warm on the outside.

Its not that it wont work, its just not optimal.

You could incubate jars without lids too! but why increase your risk of contamination?


--------------------
Uncleluke, getting his assbeat, then he tries to delete it
http://www.shroomery.org/forums/showflat.php/Number/6355469#Post6355469
Tomato-Faced Banez
http://www.shroomery.org/forums/showflat.php/Number/5933438#Post5933438
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OfflineDem_Bones
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3944544 - 03/20/05 02:44 PM (19 years, 12 days ago)

Quote:

Psychoslut said:
The jars were at 120 degrees f for 6 days, and the new ones are growing as normal,




i call total bull shit on this post , and even if by some miracle and,or it not really being 120 for 6 days , thy would not be growing normal there would be mushroom piss all in that jar any one who has grown grain spawn and let it get too warm like 95 or let it sit in the jar too long has seen the yellow liquid waste build up on side and bottom of the jar


--------------------
At the bottom of this page is a search bar to search all the post and threads on the forum so you can see all the other 500 people who asked the same thing.use the top search too it has shroomery approved FAQs USE IT YOU CAN DO IT !

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OfflineMushroomFriend
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Re: Death at 106f is bull shit [Re: XTCollection]
    #3944561 - 03/20/05 02:48 PM (19 years, 12 days ago)

You sound like a sane person to me XT! :laugh: :grin:


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Offlinehyphae
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Re: Death at 106f is bull shit [Re: IGnosticAbhorI]
    #3944785 - 03/20/05 03:26 PM (19 years, 12 days ago)

Quote:

IGnosticAbhorI said:
It's 96F and 106F for spores...

And i know that dried out myc. can be put it statis and lost for a long time...Not so sure about heat though, it the metal was Burning ass hot then it really was 120F...if not, then ur themometer was wrong
:shrug:

-Gnostic



It is 106F. where thermo death limits can occur in myc, spores are appromimately 50C. (122F.) where they can become compromised. Nothing in this world is really set in stone 106F. is just a number where the influence of heat has showed to be harmful to certain strains. Not bullshit it's a fact it's how you precieve what is being said that can cause confusion at times IME. GL guys glad to hear the myc made it through the mishap! :wink:  :thumbup:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
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Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineIGnosticAbhorI
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Re: Death at 106f is bull shit [Re: hyphae]
    #3944950 - 03/20/05 04:00 PM (19 years, 12 days ago)

Hmmm...Kind of like the whole ordeal with the "floaters" Gotcha
8-) Thankx hyphae :thumbup:

-Gnostic

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OfflineMushroomFriend
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Re: Death at 106f is bull shit [Re: hyphae]
    #3945125 - 03/20/05 04:32 PM (19 years, 12 days ago)

Quote:

hyphae said:
Quote:

IGnosticAbhorI said:
It's 96F and 106F for spores...

And i know that dried out myc. can be put it statis and lost for a long time...Not so sure about heat though, it the metal was Burning ass hot then it really was 120F...if not, then ur themometer was wrong
:shrug:

-Gnostic



It is 106F. where thermo death limits can occur in myc, spores are appromimately 50C. (122F.) where they can become compromised. Nothing in this world is really set in stone 106F. is just a number where the influence of heat has showed to be harmful to certain strains. Not bullshit it's a fact it's how you precieve what is being said that can cause confusion at times IME. GL guys glad to hear the myc made it through the mishap! :wink:  :thumbup:




Making a lot of sense! :laugh: :thumbup:


--------------------

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Offlinehyphae
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Re: Death at 106f is bull shit [Re: MushroomFriend]
    #3945157 - 03/20/05 04:37 PM (19 years, 12 days ago)

Quote:

MushroomFriend said:
Quote:

hyphae said:
Quote:

IGnosticAbhorI said:
It's 96F and 106F for spores...

And i know that dried out myc. can be put it statis and lost for a long time...Not so sure about heat though, it the metal was Burning ass hot then it really was 120F...if not, then ur themometer was wrong
:shrug:

-Gnostic



It is 106F. where thermo death limits can occur in myc, spores are appromimately 50C. (122F.) where they can become compromised. Nothing in this world is really set in stone 106F. is just a number where the influence of heat has showed to be harmful to certain strains. Not bullshit it's a fact it's how you precieve what is being said that can cause confusion at times IME. GL guys glad to hear the myc made it through the mishap! :wink:  :thumbup:




Making a lot of sense! :laugh: :thumbup:




And by "you" I meant any of us. :wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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InvisiblePsychoslut
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Re: Death at 106f is bull shit [Re: Dem_Bones]
    #3948559 - 03/21/05 11:29 AM (19 years, 11 days ago)

Newb, I have no reason to lie about this.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Offlinethenewguy05
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Re: Death at 106f is bull shit [Re: Psychoslut]
    #3948677 - 03/21/05 11:54 AM (19 years, 11 days ago)

I incubate my jars at 95 and i've never had any problems drying out or colonization.i totally agree with letting the jars cool right the hell down cuz there is no point in wasteing the spores. if you get away with it that's great but my ass it waitin'.

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