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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Clash of the Titans
    #3941617 -

Who do ya think is going to win?

I'm making a time lapse video of the whole bloody battle to come. It will be interesting when the two collide.
RR

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Re: Clash of the Titans [Re: RogerRabbit]
    #3941631 -

Tough call. I really want to see the vid when its done though :cool:

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Re: Clash of the Titans [Re: MrMaddHatter]
    #3942074 -

I think the green will win.

But I'm rooting for the home team.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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Re: Clash of the Titans [Re: scatmanrav]
    #3942206 -

the perfect fungi will win but it will leave the mycelium unusable


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

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Re: Clash of the Titans [Re: RogerRabbit]
    #3953029 -

Well, the battle is raging and they're not even touching each other. The mushroom mycelium has formed a solid line approaching the contaminant. Note the irregular leading edge of the mycelium on the opposite side of the contam as opposed to the defensive line on the contaminant side. Also note the contaminant has stopped growing and is losing vigor. This is not what I expected to happen at all. Is it chemical warfare? Psychological warfare?

By the way, the mushroom mycelium isn't Cubensis. It's Reishi, a very powerful medicinal.
RR

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Re: Clash of the Titans [Re: RogerRabbit]
    #3955608 -

That is friggin cool. Can't wait to see in a few more days.

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Re: Clash of the Titans [Re: Suntzu]
    #3966921 -

:eek:

I'd love to see that video!! Really a titans clash :thumbup:


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:whatwhat:

There's no better way to rock out than with your cock out!!

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Re: Clash of the Titans [Re: Colonel Kurtz Ph.D]
    #3967880 -

Sick.


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It's like a koala bear crapped a rainbow in my brain!

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Re: Clash of the Titans [Re: RogerRabbit]
    #3973104 -

Maybe they will live in harmony.


Is this and intentional experiment or and accidental contamination?

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Re: Clash of the Titans [Re: TheHateCamel]
    #3973341 -

Accidental. I only wanted a time-lapse movie of a petri dish colonizing, but the darn lid kept fogging up with condensation from the bright lights needed for photography. The cure was to remove the parafilm from the edges of the petri dish. That allowed the contaminant to slip in under the edge. Both the contaminant and the Reshi have started to grow again. After 48 hours of staring each other down, I noticed this morning the green had expanded 1mm toward the reishi. Within two hours, the reshi had jumped 2mm toward the contaminant. I'll post pix when I get back to my other computer where the video captures are being saved. It would appear the battle is about to begin. Two video cameras on tripods are recording every activity in this dish 24/7. I can't wait to see the outcome.
RR
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #3974879 -

Here's the latest. Note the formerly straight line of mycelium facing the contaminant has started to curve in a way that indicates it might be preparing to encircle the penicillium. Note also the contaminant has grown slightly after several days of apparent dormancy.
RR



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Re: Clash of the Titans [Re: RogerRabbit]
    #3979068 -

Awesome:thumbup:

-Gnostic


--------------------
For the sake of those seeking knowledge.
I've provided some in my signature.
Double Tek Pictorial
Getting the most out of your casings!, A pinning strategy.
Soaking wbs - The Final Answer by Agar
50/50 Coco Coir versus 50/50 Peat Moss.

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Re: Clash of the Titans [Re: IGnosticAbhorI]
    #3979360 -

very cool...intersted in seeing what happens.

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Re: Clash of the Titans [Re: smellycat]
    #3979645 -

for a second there rog, you started sounding like a commentator

...i thought that looked like penicillium-
my guess:
i think the pen is mightier than the sword


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Re: Clash of the Titans [Re: psilocyben]
    #3980374 -

Awesome! Any updates today?

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Re: Clash of the Titans [Re: ForbiddenFruit]
    #3980631 -

ForbiddenFruit said:
Awesome! Any updates today?



--------------------
Tradelist
My Ethnobotanical Garden
Cup Greenhouse Tek. Make small sturdy pots (With detachable grenhouse tops) with cups.


Your human friend will have to dose atropine uninformed, I will not spread information that may harm a cat. - Wiccan_Seeker

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Re: Clash of the Titans [Re: ForbiddenFruit]
    #3982266 -

The Reishi, after sitting and gathering strength for a few days has begun to grow toward the contaminant again. It appears to be attempting to isolate and circle the enemy.
RR





--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #3982670 -

Going in for the attack! :grin:

The green doesn't seem to have grown very much during conlonization. ?strange?

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Re: Clash of the Titans [Re: MrMaddHatter]
    #3985455 -

I have a feeling the white contam is going to cover the green one. Keep us updated!

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Re: Clash of the Titans [Re: ForbiddenFruit]
    #3985814 -

I think the green is invisibly growing , i think its fighting invisibly where the myc stopped at.

-Sub :whistling:


--------------------
Tradelist
My Ethnobotanical Garden
Cup Greenhouse Tek. Make small sturdy pots (With detachable grenhouse tops) with cups.


Your human friend will have to dose atropine uninformed, I will not spread information that may harm a cat. - Wiccan_Seeker

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Re: Clash of the Titans [Re: sublimistri]
    #3986290 -

yup. Pennicilum has a reputation for unleashing some nasty mycotoxins on its environment.


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It's like a koala bear crapped a rainbow in my brain!

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Re: Clash of the Titans [Re: BloodNOil]
    #3988193 -

But look at it. It's all boxed up and can't get away. I've never seen it go this long without forming satellite colonies. The reishi is definitely holding its own.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #3989643 -

*adds thread to favorites*

cant wait to see the outcome. GoOoooo reishi.

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Re: Clash of the Titans [Re: RogerRabbit]
    #3992284 -

The Reishi is on the attack it would appear.





--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #3992419 -

Hello, all!

I know I'm a Newbie here but

A) I had to bust my cherry
B) I have some experience with moulds in culture
C) I'm interested in the way Basidiomycetes behave on agar, so

What colour is the reverse of the "green" colony? The obverse looks Olive/~Brown to me, suggesting Cladosporium, not Penicillium. If the reverse is dark this is most certainly the case and you may or may not have a viable culture. Some Clado. spp. will restrict other fungi while not necessarily inhibiting their ability to fruit.

Hope this is a helpful first post.

Cheers!  :cheers:

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Re: Clash of the Titans [Re: LilFluffyClouds]
    #3992814 -

it's penicillium, alright.
though my exp. with it has been blueish, though i have never watched it culture on agar.

i can't wait to see what happens next episode.
thanks, RR


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Re: Clash of the Titans [Re: psilocyben]
    #3993390 -

It may possibly be aspergillus or even Cladosporium, but I'm filming the action for a time lapse video and I don't want to disturb the placement of the dish to get a microscopic identification on the contaminant. It was a green color earlier, but has since faded to what you see. If you'll look at some of the earlier pictures, the blue/green color is a bit more distinct.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #4000259 -

Very interesting !

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

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Re: Clash of the Titans [Re: RogerRabbit]
    #4001975 -

RogerRabbit said:
It may possibly be aspergillus or even Cladosporium, but I'm filming the action for a time lapse video and I don't want to disturb the placement of the dish to get a microscopic identification on the contaminant.  It was a green color earlier, but has since faded to what you see.  If you'll look at some of the earlier pictures, the blue/green color is a bit more distinct.
RR


Ahhhh, true true...

In the first couple of pix the colony is like a deep aquamarine...? I've seen both Asp. and Pen. express this colour. Sometimes Clado will also look this way VERY early and depending upon media and lighting (I'm used to MEA and halogen/fluorescents).

It looks as if the exercise is more a matter of entertainment than anything else so it doesn't really matter, and you can't really ID a mould based on colony morphology alone... You're right to not disturb the plate and invite satellite colonies.

If you're interested in naming the intruder at the end of the clash, PM me and I'll be happy to look at her under my 'scope.


Cheers!  :beer:

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Re: Clash of the Titans [Re: LilFluffyClouds]
    #4004756 -

any more updates?


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Re: Clash of the Titans [Re: RogerRabbit]
    #4008309 -

As the mycelium buildes up its defenses its going to rush over the mold, consuming it in layer of white death.

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Re: Clash of the Titans [Re: Corporal Kielbasa]
    #4008665 -

Something I have not seen so early has happened. The agar dried out after only two weeks and was starting to pull away from the edges of the petri dish like the dog eared pages on a well-worn book. The reishi and the contaminant are both fighting to the death here. I injected some sterile distilled water onto and below the agar to re-hydrate it. More pictures coming.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #4008766 -

Do you think it was from the lighting for the camera that dried it out?

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Re: Clash of the Titans [Re: Corporal Kielbasa]
    #4008929 -

I doubt it. I use halogen lighting, but it's on a cycle timer to only come on for 30 seconds every twenty minutes when the vid cap is being taken. I think it was the battle. When I get back to the lab later I'll get a pic up. Most of the sterile water has been absorbed by the agar.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #4008970 -

Halogen is some of the hotest lighting out there.

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Re: Clash of the Titans [Re: RogerRabbit]
    #4009107 -

Ok, here's what happened. The dish, unwrapped to keep it from fogging up earlier, dried out. Look at the edges peeling up at the six and twelve o'clock positions.


After adding water to the agar, it seems to have re-hydrated. Hopefully, growth will resume soon. (The first and last time I'll ever root for a contaminant)


RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #4010301 -

RogerRabbit said:
The first and last time I'll ever root for a contaminant


lol :lol:

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Re: Clash of the Titans [Re: MrMaddHatter]
    #4017440 -

The reishi has succeeded in containing and isolating the contamination by absorbing all the available water in the agar. Look at the above picture before added the water. The edges were curled up from being too dry. I haven't seen that before from dishes that were less than several months old. I will consider when growth of both organisms stopped and the dish became totally dry as the end of the experiment. I know that dry reishi mycelium will start growing again after at least two years. I don't know how long the contaminant would remain viable.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #4040000 -

Any updates, Roger?

Here is another "clash":


where Pleurotus citrinopileatus is winning the battle.. and growing over the mold...

:sun:

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Re: Clash of the Titans [Re: Speeker]
    #4040239 -

even if the white mycellium wins, would you still use it to innoc a cake? or is it unsafe?

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Re: Clash of the Titans [Re: Abermelin]
    #4040665 -

The experiment was terminated per the above post. The end was somewhat anticlimactic in that the reishi sucked up all the available moisture from the agar, thereby starving the contaminant and then going into some sort of 'hibernation' where I suppose evolution has taught it that it can outlast its enemies. There was no spectacular battle to the finish as I had hoped to film. Bummer.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Clash of the Titans [Re: RogerRabbit]
    #4054833 -

thats insane!


--------------------
:royalrainbow:I love rolling... my Katamari!!!:royalrainbow:

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Re: Clash of the Titans [Re: FleaAccela]
    #4355317 -


Is the video ready?

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Re: Clash of the Titans [Re: wideweb]
    #4371486 -

I'd watch that!

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Re: Clash of the Titans [Re: BetaDelta]
    #7855221 -

that is fucking amazing...you can see that the myc is trying not to go near it

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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