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Offlinebillbo
Stranger
Registered: 07/06/03
Posts: 4
Last seen: 5 years, 6 months
spore syringe still good?
    #3931162 - 03/17/05 05:46 PM (12 years, 3 months ago)

Hey fella's

Question I have is I have had a spore syringe in my fidge for a bit over two years. Many reasons...I was wondering if it's still viable. I read the faq's and know that some stay good for more than two years. The syringe have never been out since being placed there and I had good results with the other syringe that I ordered with it (fruited a bit over two years ago), the strain is Golden teachers. I would like to hear from someone who has had experience with using spores stored for long periods or know the answer. I could always order more because they are so cheap but would like to use this syringe if possible. I wouldn't like to sterilize the jars only to find out that all the spores are dead and don't colonize the jars. :blush:

What would you do? :confused: Thanks for your time.

peace


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Offlinebillbo
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Registered: 07/06/03
Posts: 4
Last seen: 5 years, 6 months
Re: spore syringe still good? [Re: billbo]
    #3931170 - 03/17/05 05:48 PM (12 years, 3 months ago)

.


Edited by billbo (03/17/05 05:54 PM)


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 1 day, 13 hours
Trusted Cultivator
Re: spore syringe still good? [Re: billbo]
    #3931242 - 03/17/05 06:05 PM (12 years, 3 months ago)

If your syringe still looks good, you can inoculate one jar, see what it looks like in a week, and if you see signs of life you could go back and do your other jars. Good Luck. Curious to know if it works. Let me know.Peace,Love,Happiness and Harmony. :heart:Hippie Chick :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Offlinesludge
blunt rapter

Registered: 02/23/05
Posts: 707
Loc: somewhere in usa
Last seen: 11 years, 11 months
Re: spore syringe still good? [Re: billbo]
    #3931246 - 03/17/05 06:05 PM (12 years, 3 months ago)

just try it that is something no one can answer go for it,50 50 shot,i mean either you trow it away or you use it i think i would use it


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be


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InvisibleMrMaddHatter
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Registered: 06/07/02
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Re: spore syringe still good? [Re: billbo]
    #3931249 - 03/17/05 06:06 PM (12 years, 3 months ago)

Quote:

billbo said:
What would you do? :confused: Thanks for your time.




I would try it out on two or three test jars. If it grows then  :thumbup:

If not then :thumbdown:


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Invisibleagar
old hand
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Registered: 11/21/04
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Re: spore syringe still good? [Re: billbo]
    #3931251 - 03/17/05 06:06 PM (12 years, 3 months ago)

The way to find out is to innoc whatever & see what happens. Liquid culture would be ideal.


--------------------


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 5 months
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Re: spore syringe still good? [Re: billbo]
    #3931257 - 03/17/05 06:09 PM (12 years, 3 months ago)

Try them out. Probably still good, though maybe a bit slow.

I have successfully reused some jars that I knocked up once with a culture i was testing that didnt take, they took later with the other strain and were spawned to bulk and fruited fine. Grain though, dont know if your talking PF Tek. And obviously older jars may have altered water content (drier) and more chance of contams. But properly sterilized jars should keep well for a while, grain at least..again dont know about PF Tek.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinebillbo
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Registered: 07/06/03
Posts: 4
Last seen: 5 years, 6 months
Re: spore syringe still good? [Re: scatmanrav]
    #3931300 - 03/17/05 06:19 PM (12 years, 3 months ago)

Thanks for all the quick replies. I think that inoculating a couple jars will do the trick. At least I'll know if it's still viable without wasting effort on them all.

peace


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OfflineMages
Spaz
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Registered: 12/13/03
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Re: spore syringe still good? [Re: billbo]
    #3931420 - 03/17/05 06:47 PM (12 years, 3 months ago)

Have you thought about doing a honey tek first?

http://www.shroomery.org/index/par/8005
http://www.shroomery.org/index/par/7996

If it grows a blob of mycelium in the water, you have a viable culture to inoculate lots of jars from. If it doesn't, you haven't killed yourself making the jars.


--------------------
- Mages

Ignorance killed the cat; curiosity was framed.
- C.J. Cherryh


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Offlinedr0mni
My Own Messiah
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Registered: 08/21/04
Posts: 2,921
Loc: USF Tampa, Fl
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Re: spore syringe still good? [Re: Mages]
    #3932187 - 03/17/05 09:25 PM (12 years, 3 months ago)

yeah, i just came across a B+ syringe that's supposedly over a year old with only 2cc left in it. It was sitting in room temperature in a friends pants in his closet the whole time. And the damn syringe doesn't even have threads to screw a new needle on to (or any needle for that matter).

I mean, I've already got enough Amazonian liquid cultures to last me until I can learn to make my own spore syringes, but it'd be kinda cool to grow two strains...


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 4 years, 5 months
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Re: spore syringe still good? [Re: dr0mni]
    #3932206 - 03/17/05 09:28 PM (12 years, 3 months ago)

Squirt it on agar or in a liquid culture in a glove box..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Agar

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