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Offlinejohnhenry
Minderbinder
Registered: 02/22/05
Posts: 214
Loc: capital-evil empire
Last seen: 1 year, 4 months
greenish/gray mold in mycobag-help!
    #3920789 - 03/15/05 01:47 PM (11 years, 8 months ago)

AFOAF injected 4 grain myco bags 15 days ago. 2 with b+, one with pe and one with huatla all from THE. The B+ are doing ok, healthy white growth. PE seems to be doing ok, although very slow, but the bag with huatla is forming a greenish/gray mold that looks like mycellium, but wrong color obviously. They seperated this bag from the rest and will discard if what they suspect is true- contamination?? 1. What is the cause of this? 2. Will it contaminate the other bags which seem to be healthy? What can be done to prevent this, do they have to start over, and does it have anything to do with temperature? the bags can't get much past 80deg.f do to growing limitations. Help


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: greenish/gray mold in mycobag-help! [Re: johnhenry]
    #3921123 - 03/15/05 03:19 PM (11 years, 8 months ago)

It happened because a mold spore was introduced to the substrate. Any spores will grow on the substrate, in our hobby we try and eliminate all spores EXCEPT the one were trying to grow. Somewhere along the line, that didnt happen. It could have been the bag wasnt sterilized properly, sealed properly, inoculated properly, handled properly, stored properly. It could have also been the syringe. Which if thats the case (if more bags/jars come out this way from the syringe but other strains turn out fine for you then you know its not you or the bags, but the syringe) they'll replace it. Before looking at that though, think about (if you made the bags, if you didnt, and this is the issue, theyll replace those too usually) if they were sterilized and sealed correctly. Did you wipe the bags with alcohol before injecting? Did you move the bags while the hole was in there sucking air in and out with contams? Perhaps not seal it well with tape? Maybe didnt sterilize the needle? Maybe incubator is in a dirty moldy place? Thats usually not an issue but you never know.

Sometimes contams just happen too...1 out of 5 isnt so bad...you want more like 1 out of 10 or 20 or more but bags are a little more difficult to because the suck in air whenever you move them and I've just always found them to be a pain.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineMontyRev
Stranger
Registered: 01/22/05
Posts: 96
Last seen: 10 years, 8 months
Re: greenish/gray mold in mycobag-help! [Re: scatmanrav]
    #3921246 - 03/15/05 03:57 PM (11 years, 8 months ago)

cant you store them anywhere?


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: greenish/gray mold in mycobag-help! [Re: MontyRev]
    #3921282 - 03/15/05 04:06 PM (11 years, 8 months ago)

I wouldnt stick them up in a cabnent with breads and such that might go moldy, or on the top of a fridge that never gets cleaned, or in your dirty shoe closet where your trash sits and gets changed once a month.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinejohnhenry
Minderbinder
Registered: 02/22/05
Posts: 214
Loc: capital-evil empire
Last seen: 1 year, 4 months
Re: greenish/gray mold in mycobag-help! [Re: scatmanrav]
    #3921376 - 03/15/05 04:32 PM (11 years, 8 months ago)

what are the chances the other bags will contaminate?


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OfflineMontyRev
Stranger
Registered: 01/22/05
Posts: 96
Last seen: 10 years, 8 months
Re: greenish/gray mold in mycobag-help! [Re: johnhenry]
    #3922876 - 03/15/05 10:28 PM (11 years, 8 months ago)

i think you should toss a contaminated bag rather than keep it around the healthy ones. the risk for contamination is greatly increased.

what if you keep it in your dirty show closet in a clean rubbermaid bin?


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Amazon Shop for: ½ Pint Jars, Mycobags

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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