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OfflineSensimillia
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Registered: 03/10/05
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Quick Question...
    #3901528 - 03/11/05 10:32 AM (12 years, 20 days ago)

Is it possible to make a spore syringe from a dry closed cap specimen. Any help will be greatly appreciated...thanks


Edited by Sensimillia (03/11/05 10:40 AM)


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Offlinemeehi
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Re: Quick Question... [Re: Sensimillia]
    #3902312 - 03/11/05 01:21 PM (12 years, 20 days ago)

Shame no one is replying... well to my knowledge no its not, sorry :frown:


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OfflineEdge
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Registered: 04/13/03
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Re: Quick Question... [Re: Sensimillia]
    #3902607 - 03/11/05 02:08 PM (12 years, 20 days ago)

a spore syringe, no. in order to make a spore syringe you have to let the cap of a mushroom open up fully, cut off the cap as close as you can w/ a sterile blade, and then place it on sterile tinfoil or index card or something to make a spore print. leave it there for about 24 hours.

then from a spore print you can make a syringe a multitude of ways, check out the growing articles on this site for different methods.

but no, you cant make a spore syringe from a dry closed cap because
1) the cap is closed, therefore the spores cant drop
2) the cap should be fresh and open to make a spore print (not dry)


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Offlinethenewguy05
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Re: Quick Question... [Re: Edge]
    #3902684 - 03/11/05 02:17 PM (12 years, 20 days ago)

i've read people takeing dried up shrooms from the bottom of a bag and putting it in a karo solution. so i guess you could make a mycellium syringe. high contamination risk though.


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OfflineEdge
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Re: Quick Question... [Re: thenewguy05]
    #3902710 - 03/11/05 02:21 PM (12 years, 20 days ago)

yeah its definately a high contam risk. if you wanna make a mycellium syringe you should do it with freshly picked mushies. the difference being, Sensimillia, that a SPORE syringe has spores in it and a MYCELLIUM syringe has mycellium in it... and a mycellium syringe will cause faster growth in a jar than a spore syringe.


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InvisibleFloydseamus
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Re: Quick Question... [Re: thenewguy05]
    #3905007 - 03/11/05 11:34 PM (12 years, 20 days ago)

Quote:

thenewguy05 said:
i've read people takeing dried up shrooms from the bottom of a bag and putting it in a karo solution. so i guess you could make a mycellium syringe. high contamination risk though.




I would think that if someone used dried up shrooms from the bottom of the bag, they were actually getting a little bit of spores.... If mycelium is dried, it is dead. I dont see any possible way of getting any growth unless the person were to get lucky and get some spores from that dried material... Its just not possible. Once again, someone correct me if im wrong. Peace


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Invisibledog
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Re: Quick Question... [Re: Sensimillia]
    #3905081 - 03/11/05 11:58 PM (12 years, 20 days ago)

If you were to use a scalpel to open that dried cap and remove some of the insides, and put it on an agar plate, I think you would get a few spores on there and they would germinate. (provided they were viable, of course).

The contam risk is too high for liquid culture.


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